Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2025-01-03T19:15:08.102Z Has data issue: false hasContentIssue false
  • English
  • Français

Gene cloning

Published online by Cambridge University Press:  13 July 2009

Noreen E. Murray
Get access Rights & Permissions [Opens in a new window]

Abstract

The experimental observations and techniques upon which the ‘Recombinant DNA’ revolution was founded are introduced; these often came from esoteric research, but nevertheless have promoted a dramatic change in both biotechnology and our molecular understanding of eukaryotic organisms. The basic approaches to the cloning and analysis of foreign genes in E. coli are described, followed by some indication of how the technologies continue to evolve, thereby enabling the current attack on major problems in agriculture and medicine.

Type
Research Article
Information
European Review , Volume 4 , Issue 4 , October 1996 , pp. 357 - 370
Copyright
Copyright © Academia Europaea 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1.Murray, K. (1976) Biochemical manipulation of genes. Endeavour XXXV, 129–133.CrossRefGoogle Scholar
2.Old, R. W. and Primrose, S. B. (1994) Principles of Gene Manipulation—An Introduction to Genetic, Engineering, 5th Ed, Blackwell Scientific, Oxford.Google Scholar
3.Kaiser, K., Murray, N. E. and Whittaker, P. A. (1995) DNA Cloning Vol. I, Edition II, edited by Glover, D. M. and Hames, B. D., IRL Press.Google Scholar
4.Hohn, B. and Collins, J. (1988) Ten years of cosmids. Tibtech 6, 293–298.CrossRefGoogle Scholar
5.Nasmyth, K. and Sulston, J. (1987) High-altitude walking with YACs. Nature 328, 380–381.CrossRefGoogle ScholarPubMed
6.Venter, J. C., Smith, H. O. and Hood, L. (1996) A new strategy for genome sequencing. Nature 381, 364–365.CrossRefGoogle ScholarPubMed