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Introducing a new recruitment approach to sample collection for genetic association studies in opioid dependence

Published online by Cambridge University Press:  16 April 2020

Petra Franke ,
Birgit Wendel ,
Michael Knapp ,
Sibylle G. Schwab ,
Daniela Neef ,
Wolfgang Maier ,
Dieter B. Wildenauer  and
Margret R. Hoehe
Petra Franke*
Affiliation:
Department of Psychiatry, University of Bonn, Sigmund-Freud-Str. 25, 53105Bonn, Germany
Birgit Wendel
Affiliation:
Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany
Michael Knapp
Affiliation:
Institute of Medical Biometry, Informatics and Epidemiology, University of Bonn, Bonn, Germany
Sibylle G. Schwab
Affiliation:
Department of Psychiatry, University of Bonn, Sigmund-Freud-Str. 25, 53105Bonn, Germany
Daniela Neef
Affiliation:
Department of Psychiatry, University of Bonn, Sigmund-Freud-Str. 25, 53105Bonn, Germany
Wolfgang Maier
Affiliation:
Department of Psychiatry, University of Bonn, Sigmund-Freud-Str. 25, 53105Bonn, Germany
Dieter B. Wildenauer
Affiliation:
Department of Psychiatry, University of Bonn, Sigmund-Freud-Str. 25, 53105Bonn, Germany
Margret R. Hoehe
Affiliation:
Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany Max Planck Institute for Molecular Genetics, Berlin, Germany
*
*E-mail address: [email protected]
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Abstract

Objective. –

In a modified case–control association study we tested the assumption that two polymorphisms (A118G in exon 1 and IVS2+31 in intron 2) of the human μ-opioid receptor gene (OPRM1) confer susceptibility to opioid dependence.

Methods. –

In contrast to classical case–control studies both groups, opioid dependent cases and non-opioid dependent controls were recruited from individuals who have had access to drugs including opioids and who had been sentenced for violation of the “Dangerous Drugs Act” in Germany.

Results. –

For the two allelic variants of OPRM1 under study we did not find evidence for association with opioid dependence.

Conclusions. –

Despite absence of association we think that this recruitment approach introduced here, is useful since it putatively offers a more adequate matching for case–control association studies of opioid dependent individuals.

Keywords

AddictionOpioidsOPRM1PolymorphismCase–control study
Type
Original article
Information
European Psychiatry , Volume 18 , Issue 1 , February 2003 , pp. 18 - 22
Copyright
Copyright © Éditions scientifiques et médicales Elsevier SAS 2003

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References

Enoch, MA, Goldman, DGenetics of alcoholism and substance abuse. Addict Disord 1999;22:289–99.Google ScholarPubMed
Kendler, KS, Karkowski, LM, Neale, MC, Prescott, CAIllicit psycho-active substance use, heavy use, abuse, and dependence in a US population-based sample of male twins. Arch Gen Psychiatry 2000; 57:261–9.CrossRefGoogle Scholar
Sora, I, Takahashi, N, Funada, M, Ujike, H, Revay, RS, Donovan, DMet al. Opiate receptor knockout mice define mu receptor roles in endogenous nociceptive responses and morphine-induced analgesia. Proc Natl Acad Sci USA 1997;94:1544–9.CrossRefGoogle ScholarPubMed
Uhl, GR. Molecular genetics of substance abuse vulnerability: a current approach. Neuropsychopharmacol 1999;20:3–9.CrossRefGoogle ScholarPubMed
Uhl, GR, Sora, I, Wang, ZThe µ opiate receptor as a candidate gene for pain: polymorphisms, variations in expression, nociception, and opiate responses. Proc Natl Acad Sci USA 1999;96:7752–5.CrossRefGoogle Scholar
Chen, Y, Fan, Y, Liu, J, Mestek, A, Tian, M, Kozak, CA, Yu, LMolecular cloning, tissue distribution and chromosomal localization of a novel member of the opioid receptor gene family. FEBS Lett 1994;27: 279–83.CrossRefGoogle Scholar
Wang, JB, Johnson, PS PersicoAM, Hawkins, AL, Griffin, CA, Uhl, GRHuman mu opiate receptor. cDNA and genomic clones, pharmaco-logic characterization and chromosomal assignment. FEBS Lett 1994;338:217–22.CrossRefGoogle Scholar
Mayer, P, Höllt, VAllelic and somatic variations in the endogenous opioid system of humans. Pharmacol Ther 2001;91:167–77.CrossRefGoogle ScholarPubMed
Gelernter, J, Kranzler, H, Cubells, JGenetics of two mu opioid receptor gene (OPRM1) exon I polymorphisms: population studies, and allele frequencies in alcohol- and drug-dependent subjects. Mol Psychiatry 1999;4:476–83.CrossRefGoogle ScholarPubMed
Bond, C, LaForge, KS, Tian, M, Melia, D, Zhang, S, Borg, Let al. Single-nucleotide polymorphism in the human mu opioid receptor gene alters b-endorphin binding and activity: possible implications for opiate addiction. Proc Natl Acad Sci USA 1998;95:9608–13.CrossRefGoogle ScholarPubMed
Hoehe, MR, Köpke, K, Wendel, B, Rohde, K, Flachmeier, C, Kidd, KKet al. Sequence variability and candidate gene analysis in complex disease: association of µ opioid receptor gene variation with substance dependence. Hum Mol Genet 2000;9:2895–908.CrossRefGoogle Scholar
Buchholz, KK, Cadoret, R, Cloninger, CR, Dinwiddie, SH, Hessel-brock, VM, Nurnberger, JIet al. A new, semistructured psychiatric interview for use in genetic linkage studies: a report on the reliability of the SSAGA. J Stud Alcohol 1994;55:149–58.CrossRefGoogle Scholar
Keppel, S, Dilg, C, Franke, PTest-Retest und Interrater-Reliabilität des Semistrukturierten Interviews zur Genese von Alkohol- und Drogen-abhängigkeit (SIGAD). Suchtmedizin 2001;3:217–24.Google Scholar
American Psychiatric Association (APA). Diagnostic and statistical manual of mental disorders. 3rd ed. Washington, DC: American Psychiatric Association; 1987 [Revised].Google Scholar
Wendel, B, Hoehe, MRThe human mu opioid receptor gene: 5’ regulatory and intronic sequences. J Mol Med 1998;76:525–32.CrossRefGoogle ScholarPubMed
Devlin, B, Risch, NA comparison of linkage disequilibrium measures for fine-scale mapping. Genomics 1995;29:311–22.CrossRefGoogle ScholarPubMed
Bergen, AW, Kokoszka, J, Peterson, R, Long, JC, Virkkunen, M, Lin-noila, Met al. Mu opioid receptor gene variants: lack of association with alcohol dependence. Mol Psychiatry 1997;2:490–4.CrossRefGoogle ScholarPubMed
Sander, T, Gscheidel, N, Wendel, B, Samochowiec, J, Smolka, M, Rom-melspacher, Het al. Human mu-opioid receptor variation and alcohol dependence. Alcohol Clin Exp Res 1998;22:2108–10.Google ScholarPubMed
Town, T, Abdullah, L, Crawford, F, Schinka, J, Ordorica, PI, Francis, Eet al. Association of a functional µ-opioid receptor allele (+118A) with alcohol dependency. Am J Med Genet 1999;88:458–61.3.0.CO;2-S>CrossRefGoogle Scholar
Franke, P, Wang, T, Nothen, MM, Knapp, M, Neidt, H, Albrecht, Set al. Nonreplication of association between mu-opioid-receptor gene (OPRM1) A118G polymorphism and substance dependence. Am J Med Genet 2001;105:114–9.3.0.CO;2-L>CrossRefGoogle ScholarPubMed
Szeto, CYK, Tang, NLS, Lee, DTS, Stadlin, AAssociation between mu opioid receptor gene polymorphisms and Chinese heroin addicts. Neuroreport 2001;12:1103–6.CrossRefGoogle ScholarPubMed
Shi, J, Hui, L, Xu, Y, Wang, F, Huang, W, Hu, GSequence variations in the mu-opioid receptor gene (OPRM1) associated with human addiction to heroin. Hum Mutat 2002;19:459–65.CrossRefGoogle ScholarPubMed
Reich, EE, Goldstein, DBDetecting association in a case-control study while correcting for population stratification. Genet Epidemiol 2001;20:4–16.3.0.CO;2-T>CrossRefGoogle Scholar
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