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Nucleotides, nucleoside sugars and UDP-glucose-4-epimerase activity in the iota-carrageenophytes Solieria chordalis and Calliblepharis jubata (Rhodophyceae)

Published online by Cambridge University Press:  01 February 1999

FABIENNE GOULARD
Affiliation:
Laboratoire d'Ecophysiologie et de Biochimie des Algues Marines, IUEM, UBO Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzane, France
MARCEL DIOURIS
Affiliation:
Laboratoire d'Ecophysiologie et de Biochimie des Algues Marines, IUEM, UBO Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzane, France
ERIC DESLANDES
Affiliation:
Laboratoire d'Ecophysiologie et de Biochimie des Algues Marines, IUEM, UBO Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzane, France
JEAN-YVES FLOC'H
Affiliation:
Laboratoire d'Ecophysiologie et de Biochimie des Algues Marines, IUEM, UBO Technopôle Brest-Iroise, Place Nicolas Copernic, 29280 Plouzane, France
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Abstract

The nucleotides and nucleoside diphosphate monosaccharides (NDP-sugars), UDP-galactose, UDP-glucose, UDP, ATP, ADP, ADP-glucose, AMP and NAD, some of which are involved in carrageenan biosynthesis, were identified in the two carrageenophytes Calliblepharis jubata and Solieria chordalis and quantified by high-performance liquid chromatography (HPLC). Of several extraction procedures evaluated, a freeze-thaw step followed by formic acid (1 M) extraction was the preferred method. The pool size of nucleotides and NDP-sugars differed between the two algal species, which may be related to their growth state. Calliblepharis, which was not growing actively, had larger pools than Solieria, which was in an active growth phase. Using an HPLC method UDP-D-glucose-4-epimerase activity was measured in cell-free extracts of both algae, for the first time in a carrageenophyte.

Type
Research Article
Copyright
© 1999 British Phycological Society

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