Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-24T13:46:34.993Z Has data issue: false hasContentIssue false
  • English
  • Français

Mechanisms of ketamine action on lipid metabolism in rats

Published online by Cambridge University Press:  19 April 2005

T. Saranteas ,
N. Zotos ,
E. Lolis ,
J. Stranomiti ,
C. Mourouzis ,
C. Chantzi  and
C. Tesseromatis
T. Saranteas
Affiliation:
University of Athens, Department of Pharmacology, Medical School, Athens, Greece General Hospital of Athens, Department of Anaesthesiology, ‘G Gennimatas’, Athens, Greece
N. Zotos
Affiliation:
University of Athens, Department of Pharmacology, Medical School, Athens, Greece
E. Lolis
Affiliation:
University of Athens, Department of Pharmacology, Medical School, Athens, Greece University of Athens, Department of Surgery, Aretaieion Hospital of Athens, Athens, Greece
J. Stranomiti
Affiliation:
General Hospital of Athens, Department of Anaesthesiology, ‘G Gennimatas’, Athens, Greece
C. Mourouzis
Affiliation:
University of Athens, Department of Pharmacology, Medical School, Athens, Greece
C. Chantzi
Affiliation:
General Hospital of Athens, Department of Anaesthesiology, ‘G Gennimatas’, Athens, Greece
C. Tesseromatis
Affiliation:
University of Athens, Department of Pharmacology, Medical School, Athens, Greece
Get access

Abstract

Summary

Background and objective: This study was conducted to determine the effect of ketamine on metabolic homoeostasis and particularly in lipoprotein lipase (LPL) activity in adipose tissue.

Methods: Sixty male Wistar rats were divided into six groups of 10 each. Group A served as controls, while Groups B–F received, respectively, ketamine 60, 80, 100, 120 and 140 mg kg−1 intraperitoneally. The animals were sacrificed 20 min after the administration of ketamine. Insulin concentrations in plasma and total cholesterol, triglyceride, high-density lipoprotein (HDL) and free fatty acid (FFA) concentrations in serum were measured. LPL activity in adipose tissue and medium-chain acyl-CoA dehydrogenase (MCAD) content in muscle were determined.

Results: FFA concentrations in serum significantly increased from the second lowest dose of ketamine. Insulin concentrations in plasma did not exhibit any significant difference between groups. MCAD levels were 0.5-fold more in Group F than in Group A, while there were no significant differences between control group and Groups B–E. Furthermore, high concentrations (120 and 140 mg kg−1) of ketamine interfered with in metabolic homoeostasis by significantly reducing LPL activity, thus elevating triglyceride concentrations in serum without affecting cholesterol and HDL metabolism.

Conclusions: Ketamine induces various metabolic effects due to changes in adipose LPL activity and MCAD levels in muscles. These findings seem to be significant only at high doses.

Keywords

ANAESTHETIC AGENTS, ketamineLIPIDS, lipoprotein lipase
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 22 , Issue 3 , March 2005 , pp. 222 - 226
Copyright
2005 European Society of Anaesthesiology

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Olivecrona T, Olivecrona G. Lipoprotein and hepatic lipase in lipoprotein metabolism. In: Betteridge DJ, ed. Lipoproteins in Health and Disease.London, UK: Arnold, 1999: 225–239.
Eckel RH. Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases. New Engl J Med 1989; 320: 1060–1068.Google Scholar
Beisiegel U. New aspect on role of plasma lipases in lipoprotein catabolism and atherosclerosis. Atherosclerosis 1996; 124: 1–8.Google Scholar
Champe P, Harvey R. Fatty acid and triglycerol metabolism. In: Champe P, Harvey R, eds. Lippincott's Biochemistry.Philadelphia, USA: J.B. Lippincott, 1996: 171–188.
Rosenblatt-Velin N, Montessuit C, Papageorgiou I, Terrand J, Lerch R. Postinfarction heart failure in rats is associated of GLUT-1 and downregulation of genes of fatty acid metabolism. Cardiovasc Res 2001; 52: 407–416.Google Scholar
Flecknell PA. Anaesthesia. In: Tuffery AA, ed. Laboratory Animals – An Introduction for Experiments.London: John Wiley, 1995: 325.
Trichilis A, Tesserommatis C, Varonos D. Changes in serum levels of quinolones in rats under influence of experimental trauma. Eur J Drug Metab Pharmacokinet 2000; 25: 73–78.Google Scholar
Saranteas T, Lolis E, Mourouzis C, Potamianou A, Tesseromatis C. Effect of losartan on insulin plasma concentrations and LPL-activity in adipose tissue of hypertensive rats. Hormone Metab Res 2003; 35: 164–168.Google Scholar
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248–254.Google Scholar
Nonogaki K. New insights into sympathetic regulation of glucose and fat metabolism. Diabetologia 2000; 43: 533–549.Google Scholar
Bartness TJ, Bamshad M. Innervation of mammalian white adipose tissue: implications for the regulation of total body fat. Am J Physiol 1998; 275: R1399–R1411.Google Scholar
Stoelting KR. Nonbarbiturate induction drugs. In: Stoelting KR, ed. Pharmacology and Physiology in Anesthetic Practice.Philadelphia, USA: Lippincott Williams & Wilkins, 1999: 148–154.
Okamoto H, Hoka S, Kawasaki T, Okuyama T, Tanahashi S. l-arginine attenuates ketamine-induced increase renal sympathetic nerve activity. Anesthesiology 1994; 81: 137–146.Google Scholar
Hindlycke M, Jansson L. Glucose tolerance and pancreatic islet blood flow in rats after intraperitoneal administration of different anesthetic drugs. Upsala J Med Sci 1992; 97: 27–35.Google Scholar
Brown CM, Layman DK. Use of ketamine–HCl anesthesia in studies of chylomicron–triglyceride metabolism in the rat. Lab Animal Sci 1990; 40: 183–185.Google Scholar
Tsopanakis C, Tesserommatis C. Cold swimming stress. Effect on serum lipids, lipoproteins and LCAT activity in male and female rats. Pharmacol Biochem Behav 1991; 38: 813–816.Google Scholar
Maignan E, Dong WX, Legrand M, Safer M, Cuche JL. Sympathetic activity in the rat: effects of anaesthesia on noradrenaline kinetics. J Autonom Nerv System 2000; 80: 46–51.Google Scholar
Berc PD, Stump DD. Mechanisms of cellular uptake of long chain free fatty acids. Mol Cell Biochem 1999; 192: 17–31.Google Scholar
Friedman G, Chajec-Shaul T, Stein O, Olivecrona T, Stein Y. Role of lipoprotein lipase in the assimilation of cholesteryl linoleyl ether by cultured cells incubated with labeled chylomicrons. Biochim Biophys Acta 1981; 666: 156–164.Google Scholar
Saxena U, Goldberg IJ. Interaction of lipoprotein lipase with glycosaminoglycans and apolipoprotein C-II: effects of free fatty acids. Biochim Biophys Acta 1990; 1043: 161–168.Google Scholar
Moan A, Eide IK, Kjeldsen SE. Metabolic and adrenergic characteristics of young men with insulin resistance. Blood Press Suppl 1996; 1: 30–37.Google Scholar