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Ischaemic preconditioning but not isoflurane prevents post-ischaemic production of hydroxyl radicals in a canine model of ischaemia–reperfusion

Published online by Cambridge University Press:  13 April 2005

Y. Gozal ,
M. Chevion ,
A. Elami ,
E. Berenshtein ,
N. Kitrossky  and
B. Drenger
Y. Gozal
Affiliation:
Hadassah University Hospital, Hebrew University Faculties of Medicine and Dental Medicine, Department of Anesthesiology and CCM, Jerusalem, Israel
M. Chevion
Affiliation:
Hadassah University Hospital, Hebrew University Faculties of Medicine and Dental Medicine, Department of Cellular Biochemistry and Human Genetics, Jerusalem, Israel
A. Elami
Affiliation:
Hadassah University Hospital, Hebrew University Faculties of Medicine and Dental Medicine, Department of Cardiothoracic Surgery, Jerusalem, Israel
E. Berenshtein
Affiliation:
Hadassah University Hospital, Hebrew University Faculties of Medicine and Dental Medicine, Department of Cellular Biochemistry and Human Genetics, Jerusalem, Israel
N. Kitrossky
Affiliation:
Hadassah University Hospital, Hebrew University Faculties of Medicine and Dental Medicine, Department of Cellular Biochemistry and Human Genetics, Jerusalem, Israel
B. Drenger
Affiliation:
Hadassah University Hospital, Hebrew University Faculties of Medicine and Dental Medicine, Department of Anesthesiology and CCM, Jerusalem, Israel
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Extract

Summary

Background and objective: Isoflurane has been shown to mimic ischaemic preconditioning (IPC). The protective effect of IPC, or applying isoflurane or perfusion with the ‘push-pull’ complex zinc–desferrioxamine (Zn–DFO) in the canine heart, was investigated.

Methods: Thirty minutes after salicylate administration (100 mg kg−1) the heart was exposed. All dogs were subjected to a 10 min left anterior descending artery occlusion followed by 2 h of reperfusion. In Group I (n = 9) isoflurane (2.5%) was administered 10 min prior to and during ischaemia. In Group II (n = 8), IPC was elicited by 5 min coronary artery occlusion, followed by 5 min of reperfusion, prior to the 10 min ischaemia. In Group III (n = 9) Zn–DFO (2.5 mg kg−1) was given 10 min prior to ischaemia. The effects of these interventions were compared to control (n = 10). Coronary sinus blood concentrations of salicylate, 2,3-dihydroxybenzoic acid (DHBA), lactate, pH and oxygen content were monitored.

Results: In the control group, 2,3-DHBA increased by 32% above the pre-ischaemic value (P < 0.05). In contrast, in the IPC hearts, a significant decrease in the production of 2,3-DHBA was observed (40% lower than baseline, P < 0.01). In the isoflurane group only a 13% (and non-significant) decrease was noticed. In the Zn–DFO group a 33% decrease was found (P < 0.01). The increase in lactate concentrations in the IPC and Zn–DFO groups was significantly smaller than that of control and isoflurane groups.

Conclusions: IPC protected the heart against the deleterious effects of reperfusion, possibly by amelioration of the level of oxygen-derived reactive species, and the complete inhibition of reactive hydroxyl radical production. Isoflurane did not prove to be as effective in reducing the free radical damage.

Keywords

MYOCARDIAL REPERFUSION INJURYANAESTHETICS INHALATION, isofluraneISCHAEMIC PRECONDITIONING, MYOCARDIALCHELATING AGENTS, zinc, desferrioxamine
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 22 , Issue 1 , January 2005 , pp. 49 - 55
Copyright
© 2005 European Society of Anaesthesiology

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References

Warltier DC, Al-Wathiqui MH, Kampine JP, Schmeling WT. Recovery of contractile function of stunned myocardium in chronically instrumented dogs is enhanced by halothane or isoflurane. Anesthesiology 1988; 69: 552–565.Google Scholar
Marijic J, Stowe DF, Turner LA, et al. Differential protective effects of halothane and isoflurane against hypoxia and reoxygenation injury in the isolated guinea pig heart. Anesthesiology 1990; 73: 976–983.Google Scholar
Luss H, Meissner A, Rolf N, et al. Biochemical mechanism(s) of stunning in conscious dogs. Am J Physiol 2000; 279: H176–H184.Google Scholar
Kanaya N, Kobayashi I, Nakayama M, et al. ATP sparing effect of isoflurane during ischaemia and reperfusion of the canine heart. Br J Anaesth 1995; 74: 563–568.Google Scholar
Kanaya N, Fujita S. The effects of isoflurane on regional myocardial contractility and metabolism in ‘stunned’ myocardium in acutely instrumented dogs. Anesth Analg 1994; 79: 447–454.Google Scholar
Kersten JR, Orth KG, Pagel PS, et al. Role of adenosine in isoflurane-induced cardioprotection. Anesthesiology 1997; 86: 1128–1139.Google Scholar
Kersten JR, Lowe D, Hettrick DA, et al. Glyburide, a KATP channel antagonist, attenuates the cardioprotective effects of isoflurane in stunned myocardium. Anesth Analg 1996; 83: 27–33.Google Scholar
Kersten JR, Schmeling TJ, Hettrick DA, et al. Mechanism of myocardial protection by isoflurane: role of adenosine triphosphate-regulated potassium (KATP) channels. Anesthesiology 1996; 85: 794–807.Google Scholar
Kersten JR, Schmeling TJ, Pagel PS, et al. Isoflurane mimics ischemic preconditioning via activation of KATP channels. Anesthesiology 1997; 87: 361–370.Google Scholar
Chevion M, Jiang Y, Har-El R, et al. Copper and iron are mobilized following myocardial ischemia: possible predictive criteria for tissue injury. Proc Nat Acad Sci 1993; 90: 1102–1106.Google Scholar
Chevion M. A site-specific mechanism for free radical induced biological damage: the essential role of redox-active transition metals. Free Radic Biol Med 1988; 5: 27–37.Google Scholar
Chevion M. Protection against free radical-induced and transition metal-mediated damage: the use of ‘pull’ and ‘push’ mechanisms. Free Radic Res Comms 1991; 12–13: 691–696.Google Scholar
Karwatowska-Prokopczuk E, Czarnowska E, Beresewicz A. Iron availability and free radical induced injury in the isolated ischaemic/reperfused rat heart. Cardiovasc Res 1992; 26: 58–66.Google Scholar
Samuni AM, Afeworki M, Stein W, et al. Multifunctional antioxidant activity of HBED iron chelator. Free Radic Biol Med 2001; 30: 170–177.Google Scholar
Berenshtein E, Vaisman B, Goldberg-Langerman C, et al. Roles of ferritin and iron in ischemic preconditioning of the heart. Mol Cell Biochem 2002; 234–235: 283–292.Google Scholar
Floyd RA, Henderson R, Watson JJ, Wong PK. Use of salicylate with high pressure liquid chromatography and electrochemical detection (LCED) as a sensitive measure of hydroxyl free radicals in adriamycin treated rats. Free Radic Biol Med 1986; 2: 13–18.Google Scholar
Onodera T, Ashraf M. Detection of hydroxyl radicals in the post-ischemic reperfused heart using salicylate as a trapping agent. J Mol Cell Cardiol 1991; 23: 365–370.Google Scholar
Halliwell B, Kaur H, Ingelman-Sundberg M. Hydroxylation of salicylate as an assay for hydroxyl radicals: a cautionary note. Free Radic Biol Med 1991; 10: 439–441.Google Scholar
Glantz L, Ginosar Y, Chevion M, et al. Halothane prevents postischemic production of hydroxyl radicals in the canine heart. Anesthesiology 1997; 86: 440–447.Google Scholar
Simpson PJ, Lucchesi BR. Free radicals and myocardial ischemia and reperfusion injury. J Lab Clin Med 1987; 110: 13–30.Google Scholar
Opie LH. Reperfusion injury and its pharmacologic modification. Circulation 1989; 80: 1049–1062.Google Scholar
Pain T, Yang XM, Critz SD, et al. Opening of mitochondrial KATP channels triggers the preconditioned state by generating free radicals. Circ Res 2000; 87: 460–466.Google Scholar
Bolli R, Patel BS, Zhu WX, et al. The iron chelator desferrioxamine attenuates postischemic ventricular dysfunction. Am J Physiol 1987; 253: H1372–H1380.Google Scholar
Karck M, Tanaka S, Berenshtein E, et al. The ‘push-pull’ mechanism to scavenge redox active transition metals: a novel concept in myocardial protection. J Thorac Cardiovasc Surg 2001; 121: 1169–1178.Google Scholar
Kashimoto S, Kume M, Ikeya K, Kumazawa T. Effects of sevoflurane and isoflurane in the post-ischaemic reperfused heart. Eur J Anaesthesiol 1998; 15: 553–558.Google Scholar