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The effect of lidocaine on neutrophil CD11b/CD18 and endothelial ICAM-1 expression and IL-1β concentrations induced by hypoxia–reoxygenation

Published online by Cambridge University Press:  28 January 2005

W. Lan ,
D. Harmon ,
J. H. Wang ,
G. Shorten  and
P. Redmond
W. Lan
Affiliation:
Cork University Hospital and University College Cork, Academic Department of Surgery, Cork, Ireland
D. Harmon
Affiliation:
Cork University Hospital and University College Cork, Department of Anaesthesia and Intensive Care Medicine, Cork, Ireland
J. H. Wang
Affiliation:
Cork University Hospital and University College Cork, Academic Department of Surgery, Cork, Ireland
G. Shorten
Affiliation:
Cork University Hospital and University College Cork, Department of Anaesthesia and Intensive Care Medicine, Cork, Ireland
P. Redmond
Affiliation:
Cork University Hospital and University College Cork, Department of Surgery, Cork, Ireland
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Extract

Summary

Background: Lidocaine has actions potentially of benefit during ischaemia–reperfusion. Neutrophils and endothelial cells have an important role in ischaemia–reperfusion injury.

Methods: Isolated human neutrophil CD11b and CD18, and human umbilical vein endothelial cell (HUVEC) ICAM-1 expression and supernatant IL-1β concentrations in response to hypoxia–reoxygenation were studied in the presence or absence of different concentrations of lidocaine (0.005, 0.05 and 0.5 mg mL−1). Adhesion molecule expression was quantified by flow cytometry and IL-1β concentrations by ELISA. Differences were assessed with analysis of variance and Student–Newman–Keuls as appropriate. Data are presented as mean ± SD.

Results: Exposure to hypoxia–reoxygenation increased neutrophil CD11b (94.33 ± 40.65 vs. 34.32 ± 6.83 mean channel fluorescence (MCF), P = 0.02), CD18 (109.84 ± 35.44 vs. 59.05 ± 6.71 MCF, P = 0.03) and endothelial ICAM-1 (146.62 ± 16.78 vs. 47.29 ± 9.85 MCF, P < 0.001) expression compared to normoxia. Neutrophil CD18 expression on exposure to hypoxia–reoxygenation was less in lidocaine (0.005 mg mL−1) treated cells compared to control (71.07 ± 10.14 vs. 109.84 ± 35.44 MCF, P = 0.03). Endothelial ICAM-1 expression on exposure to hypoxia–reoxygenation was less in lidocaine (0.005 mg mL−1) treated cells compared to control (133.25 ± 16.05 vs. 146.62 ± 16.78 MCF, P = 0.03). Hypoxia–reoxygenation increased HUVEC supernatant IL-1β concentrations compared to normoxia (3.41 ± 0.36 vs. 2.65 ± 0.21 pg mL−1, P = 0.02). Endothelial supernatant IL-1β concentrations in lidocaine-treated HUVECs were similar to controls.

Conclusions: Lidocaine at clinically relevant concentrations decreased neutrophil CD18 and endothelial ICAM-1 expression but not endothelial IL-1β concentrations.

Keywords

ANAESTHETICS, LOCAL, lidocaineENDOTHELIAL CELL, interleukins, cytokines, inflammationHYPOXIA, reoxygenationNEUTROPHIL, cytokines
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 21 , Issue 12 , December 2004 , pp. 967 - 972
Copyright
© 2004 European Society of Anaesthesiology

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