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Ropivacaine myotoxicity after single intramuscular injection in rats

Published online by Cambridge University Press:  20 January 2006

E. Amaniti ,
F. Drampa ,
K. Kouzi-Koliakos ,
D. Kapoukranidou ,
C. Pourzitaki ,
E. Tsalie  and
D. Vasilakos
E. Amaniti
Affiliation:
Aristotle University of Thessaloniki, School of Medicine, AHEPA University Hospital, Anaesthesiology Department, Thessaloniki, Greece
F. Drampa
Affiliation:
Aristotle University of Thessaloniki, School of Medicine, AHEPA University Hospital, Anaesthesiology Department, Thessaloniki, Greece
K. Kouzi-Koliakos
Affiliation:
Aristotle University of Thessaloniki, School of Medicine, Laboratory of Histology and Embryology, Thessaloniki, Greece
D. Kapoukranidou
Affiliation:
Aristotle University of Thessaloniki, School of Medicine, Laboratory of Experimental Physiology, Thessaloniki, Greece
C. Pourzitaki
Affiliation:
Aristotle University of Thessaloniki, School of Medicine, Laboratory of Pharmacology, Thessaloniki, Greece
E. Tsalie
Affiliation:
Aristotle University of Thessaloniki, School of Medicine, Laboratory of Histology and Embryology, Thessaloniki, Greece
D. Vasilakos
Affiliation:
Aristotle University of Thessaloniki, School of Medicine, AHEPA University Hospital, Anaesthesiology Department, Thessaloniki, Greece
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Summary

Background and objective: We conducted this study in order to evaluate the potential myotoxic effects of ropivacaine after single injection in rats and the time-course of the possible damage. Methods: One hundred and twenty-eight male Wistar rats were allocated to four different groups. The first three groups received intramuscular injections with ropivacaine 0.75%, ropivacaine 0.5% and normal saline, respectively, into the right tibialis anterior muscle. The fourth group received needle puncture without injection. Eight rats from each group were sacrificed 2, 4, 7 and 30 days after injection. Samples were blindly examined under light microscope for evidence of myotoxicity, scored as 0 = no damage to 3 = myonecrosis and statistically analysed. Samples obtained 7 days after injection were also examined under transmission electron microscope. Results: Ropivacaine 0.75% and ropivacaine 0.5% caused extensive destruction to muscles fibres, compared to saline or needle on days 2, 4 and 7. Statistically significant differences were found in muscle damage by drug injections among all groups except for saline vs. needle groups. Thirty days after injections all sample appearances had returned to normal. Conclusions: Ropivacaine after single intramuscular injection caused reversible muscle damage in a dose-dependent manner.

Keywords

LOCAL ANAESTHETICS, ropivacaineTOXICITY, musclesRAT
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 23 , Issue 2 , February 2006 , pp. 130 - 135
Copyright
© 2006 European Society of Anaesthesiology

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References

Hogan Q, Dotson R, Erickson S, Kettler R, Hogan K. Local anesthetic myotoxicity: A case and review. Anesthesiology 1994; 80: 942–947.Google Scholar
Foster AH, Carlson BM. Myotoxicity of local anesthetics and regeneration of the damaged muscle fibers. Anesth Analg 1980; 59: 727–736.Google Scholar
Zink W, Graf BM. Local anesthetic myotoxicity. Reg Anesth Pain Med 2004; 29: 333–340.Google Scholar
Gómez-Arnau JI, Yanguela J, Gonzalez A et al. Anaesthesia-related diplopia after cataract surgery. Br J Anaesth 2003; 90: 189–192.Google Scholar
Zink W, Seif C, Bohl JRE et al. The acute myotoxic effects of bupivacaine and ropivacaine after continuous peripheral nerve blockades. Anesth Analog 2003; 97: 1173–1179.Google Scholar
Benoit PW, Yagiela JA, Fort NF. Pharmacological correlation between local anesthetic-induced myotoxicity and disturbances of intracellular Ca2+ distribution. Toxicol Appl Pharmacol 1980; 52: 187–198.Google Scholar
Petterson N, Berggren P, Larsson M, Westman B, Hahn RG. Pain relief by wound infiltration with bupivacaine or high-dose ropivacaine after inguinal hernia repair. Reg Anesth Pain Med 1999; 24: 569–575.Google Scholar
Benoit PW, Belt WD. Destruction and regeneration of skeletal muscle after treatment with a local anaesthetic, bupivacaine (Marcaine). J Anat 1970; 107: 547–556.Google Scholar
Hall-Craggs ECB. Early ultrastructural changes in skeletal muscle exposed to local anesthetic bupivacaine (Marcaine). Br J Exp Pathol 1980; 61: 139–149.Google Scholar
Kytta J, Heinon E, Rosenberg PH et al. Effects of repeated bupivacaine administration on sciatic nerve and surrounding muscle tissue in rats. Acta Anaesthesiol Scand 1986; 30: 625–629.Google Scholar
Basson MD, Carlson BM. Myotoxicity of single and repeated injections of Mepivacaine (carbocaine) in the rat. Anesth Analg 1980; 59: 275–282.Google Scholar
Pere P, Watanabe H, Pitkanen M, Wahlstrom T, Rosenberg PH. Local myotoxicity of bupivacaine in rabbits after continuous supraclavicular bracial plexus block. Reg Anesth 1993; 18: 304–307.Google Scholar
Komai H, Lokuta AJ. Interaction of bupivacaine and tetracaine with the sarcoplasmic reticulum Ca2+ release channel of skeletal and cardiac muscles. Anesthesiology 1999; 90: 835–843.Google Scholar
Zink W, Graf BM, Sinner B, Martin E, Fink RHA, Kunst G. Differential effects of bupivacaine on intracellular Ca2+ regulation. Anesthesiology 2002; 97: 710–716.Google Scholar
Takahashi S. Local anaesthetic bupivacaine alters function of sarcoplasmic reticulum and sarcolemmal vesicles from rabbit masseter muscle. Pharmacol Toxicol 1994; 75: 119–128.Google Scholar