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Quantitative analysis of influence of sevoflurane on the reactivity of microglial cells in the course of the experimental model of intracerebral haemorrhage

Published online by Cambridge University Press:  24 May 2006

Z. Karwacki ,
P. Kowiański ,
J. Dziewiatkowski ,
B. Domaradzka-Pytel ,
B. Ludkiewicz ,
S. Wójcik ,
O. Narkiewicz  and
J. Moryś
Z. Karwacki
Affiliation:
Medical University of Gdańsk, Department of Neuroanaesthesiology, Gdańsk, Poland
P. Kowiański
Affiliation:
Medical University of Gdańsk, Department of Anatomy and Neurobiology, Gdańsk, Poland
J. Dziewiatkowski
Affiliation:
Medical University of Gdańsk, Department of Anatomy and Neurobiology, Gdańsk, Poland
B. Domaradzka-Pytel
Affiliation:
Medical University of Gdańsk, Department of Anatomy and Neurobiology, Gdańsk, Poland
B. Ludkiewicz
Affiliation:
Medical University of Gdańsk, Department of Anatomy and Neurobiology, Gdańsk, Poland
S. Wójcik
Affiliation:
Medical University of Gdańsk, Department of Anatomy and Neurobiology, Gdańsk, Poland
O. Narkiewicz
Affiliation:
Medical University of Gdańsk, Department of Anatomy and Neurobiology, Gdańsk, Poland
J. Moryś
Affiliation:
Medical University of Gdańsk, Department of Anatomy and Neurobiology, Gdańsk, Poland
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Abstract

Summary

Backgrounds: Microglial cells play an important role in the pathophysiology of intracerebral haemorrhage. We have examined the possible influence of sevoflurane on the reactivity of microglial cells during intracranial haemorrhage. Methods: Forty adult male rats were divided into two groups. All animals were anaesthetized with fentanyl, dehydrobenzperidol and midazolam. In the experimental group animals additionally received sevoflurane 2.2 vol% end-tidal concentration. Intracranial haemorrhage was produced through infusion of blood into the striatum. The microglial cell population (numerical density of immunoreactive cells and their distribution) was assessed on days 1, 3, 7, 14 and 21 after producing a haematoma using antibodies OX42 and OX6. Results: In the control group significant differences in the density of OX42-ir cells between 3rd and 7th (81.86 vs. 129.99) (95% CI: −77.99 to −18.25, P = 0.0035) and between 14th and 21st (105.36 vs. 63.81) (95% CI: 13.21 to 69.89, P = 0.006) survival days were observed. However, significant increase of percentage of amoeboid OX42-ir cells between 3rd and 7th (0.98 vs. 48.71) (95% CI: −52.17 to −43.30, P = 0.0001) and between 7th and 14th (48.71 vs. 58.47) (95% CI: −13.96 to −5.55, P = 0.0002) and then their decrease – between 14th and 21st (58.47 vs. 31.74) (95% CI: 22.52 to 30.93, P = 0.0001) days of observation were noted. In the sevoflurane groups OX42-ir cells were not found. On the 3rd day the density of OX6-ir cells in the sevoflurane group was significantly lower than that in the control group (12.39 vs. 34.57) (95% CI: −49.78 to −2.96, P = 0.02). The percentage of an amoeboid form of OX6-ir cells was significantly lower in the sevoflurane group than that in the control group (27.31 vs. 82.03) (95% CI: −72.52 to −36.92, P = 0.0001) (58.76 vs. 82.37) (95% CI: −38.81 to −8.41, P = 0.003) (42.87 vs. 81.55) (95% CI: −53.23 to −24.10, P = 0.0001) respectively for 3rd, 7th and 14th days of survival. Conclusion: Administration of sevoflurane during anaesthesia in animals with intracerebral haemorrhage evoked a decrease of activation of the microglial cells.

Keywords

ANAESTHETICS INHALATIONAL, sevofluraneMICROGLIARATCEREBRAL HAEMORRHAGE
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 23 , Issue 10 , October 2006 , pp. 874 - 881
Copyright
2006 European Society of Anaesthesiology

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