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Time course and magnitude of fluid and electrolyte shifts during recovery from high-intensity exercise in Standardbred racehorses

Published online by Cambridge University Press:  09 March 2007

Amanda Waller  and
Michael I Lindinger
Amanda Waller*
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Michael I Lindinger
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
*
*[email protected]
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Abstract

The present study characterized the fluid and electrolyte shifts that occur in Standardbred racehorses during recovery from high-intensity exercise. Jugular venous blood was sampled from 13 Standardbreds in racing condition, at rest and for 2 h following a high-intensity training workout. Total body water (TBW), extracellular fluid volume (ECFV) and plasma volume (PV) were measured at rest using indicator dilution techniques (D2O, thiocyanate and Evans Blue, respectively). Changes in TBW were assessed from measures of body mass, and changes in PV and ECFV were calculated from changes in plasma protein concentration. Exercise resulted in a 26.9% decrease in PV. At 10 min of recovery TBW and ECFV were decreased by 2.2% and 16.5% respectively, while intracellular fluid volume was increased by 7.1%. There was a continued loss of fluid due to sweating throughout the recovery period such that TBW was decreased by 3.9% at 90 min of recovery. This decrease in TBW was nearly equally partitioned between the extracellular and intracellular fluid compartments. Plasma Na+ and Cl− contents were decreased at 1 min of recovery, but not different from rest by 40 min of recovery. Plasma K+ content at 1 min post exercise was not different from the pre-exercise value; however, by 5 min of recovery K+ content was significantly decreased and it remained decreased throughout the recovery period. It is concluded that there are very rapid and large fluid and electrolyte shifts between body compartments during and after high-intensity exercise, and that full recovery of these shifts requires 90–120 min.

Keywords

equineexercise dehydrationfluid shiftelectrolytesfluid recovery
Type
Research Article
Information
Equine and Comparative Exercise Physiology , Volume 2 , Issue 2 , May 2005 , pp. 77 - 87
Copyright
Copyright © Cambridge University Press 2005

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References

1Lindinger, MI (1999) Exercise in the heat: thermoregulatory limitations to performances in humans and horses. Canadian Journal of Applied Physiology 24: 135–146.CrossRefGoogle ScholarPubMed
2Lindinger, MI, McKeen, G and Ecker, GL (2004) Time course and magnitude of changes in total body water, extracellular fluid volume, intracellular fluid volume and plasma volume during submaximal exercise and recovery in horses. Equine and Comparative Exercise Physiology 1: 131–139.CrossRefGoogle Scholar
3Kozlowski, S and Saltin, B (1964) Effect of sweat loss on body fluids. Journal of Applied Physiology 19: 1119–1124.CrossRefGoogle ScholarPubMed
4Nose, H, Mack, GW, Shi, XR and Nadel, ER (1988) Shift in body fluid compartments after dehydration in humans. Journal of Applied Physiology 65: 318–324.CrossRefGoogle ScholarPubMed
5Maw, GJ, MacKenzie, IL and Taylor, NAS (1998) Human body fluid distribution during exercise in hot, temperate and cool environments. Acta Physiologica Scandinavica 163: 297–304.CrossRefGoogle ScholarPubMed
6Cornish, BH, Wotton, MJ, Ward, LC, Thomas, BJ and Hills, AP (2001) Fluid shifts resulting from exercise in rats as detected by bioelectrical impedance. Medicine and Science in Sports and Exercise 33: 249–254.CrossRefGoogle ScholarPubMed
7Lundvall, J, Mellander, S, Westling, H and White, T (1972) Fluid transfer between blood and tissues during exercise. Acta Physiologica Scandinavica 85: 258–269.CrossRefGoogle ScholarPubMed
8Lindinger, MI, Heigenhauser, GJF, McKelvie, RS and Jones, NL (1990) Role of non-working muscle on blood metabolites and electrolytes during and following intense intermittent exercise. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 258: R1486–R1494.Google ScholarPubMed
9Lindinger, MI, Spriet, LL, Hultman, E, Putman, T, McKelvie, RS, Lands, LC et al. (1994) Plasma volume and ion regulation during exercise after low- and high-carbohydrate diets. American Journal of Physiology 266: R1896–R1906.Google ScholarPubMed
10Sjogaard, G and Saltin, B (1982) Extra- and intracellular water spaces in muscles of man at rest and with dynamic exercise. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 243: R271–R280.Google Scholar
11Hodgson, DR, McCutcheon, LJ, Byrd, SK, Brown, WS, Bayly, WM, Brengelmann, GL et al. ,(1993) Dissipation of metabolic heat in the horse during exercise. Journal of Applied Physiology 74: 1161–1170.CrossRefGoogle ScholarPubMed
12Forro, M, Cieslar, S, Ecker, GL, Walzak, A, Hahn, J and Lindinger, MI (2000) Total body water and ECFV measured using bioelectrical impedance analysis and indicator dilution in horses. Journal of Applied Physiology 89: 663–671.CrossRefGoogle ScholarPubMed
13Foldager, N and Blomqvist, CG (1991) Repeated plasma volume determination with the Evans blue dye dilution technique: the method and a computer program. Computing in Biology and Medicine 21: 35–41.CrossRefGoogle Scholar
14Chatterjee, MS, Abdel-Rahman, M, Bhandal, A, Klein, P and Bogden, J (1988) Amniotic fluid and thiocyanate in pregnant women who smoke. Journal of Reproductive Medicine 33: 417–420.Google ScholarPubMed
15Lindinger, MI, McCutcheon, LJ, Ecker, GL and Geor, RJ (2000) Heat acclimation improves regulation of plasma volume and plasma Na + content during exercise in horses. Journal of Applied Physiology 88: 1006–1013.CrossRefGoogle ScholarPubMed
16Brownlow, MA and Hutchins, DR (1982) The concept of osmolality: its use in the evaluation of “dehydration” in the horse. Equine Veterinary Journal 14: 106–110.CrossRefGoogle ScholarPubMed
17Coenen, M (1992). Water and electrolyte metabolism in horses during long-lasting exercise. In: Proceedings: Exercise Physiology and Physical Performance in Farm Animals – Comparative and Specific Aspects. Berne, Switzerland: University of Berne, pp. 21–36.Google Scholar
18Carlson, GP (1987). Hematology and body fluids in the equine athlete. In: Gillespie, JR & Robinson, NE (eds), Equine Exercise Physiology 2. Davis, CA: ICEEP Publications, pp. 393–425.Google Scholar
19Saltin, B (1964) Circulatory responses to submaximal and maximal exercise after thermal dehydration. Journal of Applied Physiology 19: 1125–1132.CrossRefGoogle ScholarPubMed
20Lindinger, MI, Geor, RJ, Ecker, GL and McCutcheon, LJ (1995) Plasma volume and ions during exercise in cool dry, hot dry, and hot humid conditions. Equine Veterinary Journal Supplement 20: 133–139.CrossRefGoogle Scholar
21Haskell, A, Nadel, ER, Stachenfeld, NS, Nagashima, K and Mack, GW (1997) Transcapillary escape rate of albumin in humans during exercise-induced hypervolemia. Journal of Applied Physiology 83: 407–413.CrossRefGoogle ScholarPubMed
22Wu, J and Mack, GW (2001) Effect of lymphatic outflow pressure on lymphatic albumin transport in humans. Journal of Applied Physiology 91: 1223–1228.CrossRefGoogle ScholarPubMed
23Kozlowski, S and Saltin, B (1964) Effect of sweat loss on body fluids. Journal of Applied Physiology 19: 1119–1124.CrossRefGoogle ScholarPubMed
24Harrison, MH, Edwards, RJ and Leitch, DR (1975) Effect of exercise and thermal stress on plasma volume. Journal of Applied Physiology 39: 925–931.CrossRefGoogle ScholarPubMed
25McKeever, KH, Hinchcliff, KW, Reed, SM and Robertson, JT (1993) Role of decreased plasma volume in hematocrit alterations during incremental treadmill exercise in horses. American Journal of Physiology 265: R404–R408.Google ScholarPubMed
26Magnusson, OG, Kaijser, L, Isberg, B and Saltin, B (1994) Cardiovascular responses during one- and two-legged exercise in middle-aged men. Acta Physiologica Scandinavica 150: 353–362.CrossRefGoogle ScholarPubMed
27Gunn, HM (1987). Muscle, bone and fat proportions and muscle distribution of Thoroughbreds and other horses. In: Gillespie, JR & Robinson, NE (eds), Equine Exercise Physiology 2. Davis, CA: ICEEP Publications, pp. 253–264.Google Scholar
28Kerr, MG and Snow, DH (1982) Alterations in haematocrit, plasma proteins and electrolytes in horses following the feeding of hay. Veterinary Record 110: 538–540.Google Scholar
29Masri, M, Freestone, JF, Wolfsheimer, KJ and Shoemaker, K (1990) Alterations in plasma volume, plasma constituents, rennin activity, and aldosterone induced by maximal exercise in the horse. Equine Veterinary Journal Supplement 9: 72–77.CrossRefGoogle Scholar
30Geor, RJ, Weiss, DJ and Smith, CM (1994) Hemorheologic alterations induced by incremental treadmill exercise in Thoroughbreds. American Journal of Veterinary Research 55: 854–861.CrossRefGoogle ScholarPubMed
31Lindinger, MI, Heigenhauser, GJ, McKelvie, RS and Jones, NL (1992) Blood ion regulation during repeated maximal exercise and recovery in humans. American Journal of Physiology 262: R126–R136.Google ScholarPubMed
32McKelvie, RS, Lindinger, MI, Heigenhauser, GJF and Jones, NL (1991) Contribution of erythrocytes to the control of the electrolyte changes of exercise. Canadian Journal of Physiology and Pharmacolgy 69: 984–993.CrossRefGoogle Scholar
33Lindinger, MI and Heingenhauser, GJF (1988) Ion fluxes during titanic stimulation in the isolated perfused rat hindlimb. American Journal of Physiology 254: R117–R126.Google Scholar
34Clausen, T (1986) Regulation of active Na + –K + transport in skeletal muscle. Physiological Reviews 66: 542–580.CrossRefGoogle Scholar
35Lang, F, Busch, GL, Ritter, M, Völkl, H, Waldegger, S, Gulbins, E et al. (1998) Functional significance of cell volume regulatory mechanisms. Physiological Reviews 78: 247–306.CrossRefGoogle ScholarPubMed
36Harris, P and Snow, DH (1988) The effects of high intensity exercise on the plasma concentration of lactate, potassium and other electrolytes. Equine Veterinary Journal 20: 109–113.CrossRefGoogle ScholarPubMed
37Harris, P and Snow, DH (1992) Plasma potassium and lactate concentrations in Thoroughbred horses during exercise of varying intensity. Equine Veterinary Journal 23: 220–225.CrossRefGoogle Scholar
38Lindinger, MI, Heigenhauser, GJF and McKelvie, RS (1995) K + and Lac - distribution in humans during and after high-intensity exercise: role in muscle fatigue attenuation? Journal of Applied Physiology 78: 765–777.CrossRefGoogle ScholarPubMed
39Clausen, T (1992) Potassium and sodium transport and pH regulation. Canadian Journal of Physiology and Pharmacology 70: (Supplement) S219–S222.CrossRefGoogle ScholarPubMed
40Gottlieb-Vedi, M, Dahlborn, K, Jansson, A and Wroblewski, R (1996) Elemental composition of muscle at rest and potassium levels in muscle, plasma and sweat of horses exercising at 20°C and 35°C. Equine Veterinary Journal Supplement 22: 35–41.CrossRefGoogle Scholar
41Hodgson, DR, McCutcheon, LJ, Byrd, SK, Brown, WS, Bayly, WM, Brengelmann, GL et al. (1993) Dissipation of metabolic heat in the horse during exercise. Journal of Applied Physiology 74: 1161–1170.CrossRefGoogle ScholarPubMed
42McCutcheon, LJ, Geor, RJ, Hare, J, Ecker, GL and Lindinger, MI (1995) Sweating rate and sweat composition during exercise and recovery in ambient heat and humidity. Equine Veterinary Journal 20: 153–157.CrossRefGoogle Scholar
43Watson, PD, Garner, RP and Ward, DS (1993) Water uptake in stimulated cat skeletal muscle. American Journal of Physiology 264: R790–R796.Google ScholarPubMed
44Schuback, K, Essen-Gustavsson, B (1998) Muscle anaerobic response to a maximal treadmill exercise test in Standardbred trotters. Equine Veterinary Journal 30: 504–510.CrossRefGoogle ScholarPubMed