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Impact of training and exercise intensity on blood antioxidant markers in healthy Standardbred horses

Published online by Cambridge University Press:  09 March 2007

Brieuc de Moffarts ,
Nathalie Kirschvink ,
Tatiana Art ,
Joël Pincemail ,
Charles Michaux ,
Karine Cayeux ,
Jean-Olivier Defraigne  and
Pierre Lekeux
Brieuc de Moffarts*
Affiliation:
Department for Functional Sciences, Faculty of Veterinary Medicine, University of Liège, Bât B42, Sart Tilman, B-4000 Liège, Belgium
Nathalie Kirschvink*
Affiliation:
Department for Functional Sciences, Faculty of Veterinary Medicine, University of Liège, Bât B42, Sart Tilman, B-4000 Liège, Belgium
Tatiana Art
Affiliation:
Department for Functional Sciences, Faculty of Veterinary Medicine, University of Liège, Bât B42, Sart Tilman, B-4000 Liège, Belgium
Joël Pincemail
Affiliation:
3Probiox SA, Belgium
Charles Michaux
Affiliation:
Department for Animal Production, Faculty of Veterinary Medicine, University of Liège, Belgium
Karine Cayeux
Affiliation:
3Probiox SA, Belgium
Jean-Olivier Defraigne
Affiliation:
Department of Cardiovascular Surgery and CREDEC, Centre Hospitalier Universitaire, University of Liège, Belgium
Pierre Lekeux
Affiliation:
Department for Functional Sciences, Faculty of Veterinary Medicine, University of Liège, Bât B42, Sart Tilman, B-4000 Liège, Belgium
*
*Nathalie. [email protected] and Brieuc [email protected]
*Nathalie. [email protected] and Brieuc [email protected]
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Abstract

This study investigated the effect of training and exercise intensity on blood antioxidant markers in six healthy Standardbred horses. Markers studied were uric acid (UA), ascorbic acid (AA), α-tocopherol, vitamin A (Vit A), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione – reduced (GSH) and oxidized (GSSG), glutathione redox ratio (GRR), copper (Cu), zinc (Zn) and selenium (Se). The horses performed a standardized exercise test (SET) when they were untrained (T0), after 4 weeks of light training (T4) and after 8 weeks of interval training (T12). Forty-eight hours after SET T4 and SET T12, a SET with run up to fatigue (TTF4 and TTF12) was performed. Maximal oxygen consumption (V˙O2max) was determined within 3–5 days after TTF4 and TTF12. At each test (SET T(i) and TTF(i)), venous blood was sampled at rest (R), peak-exercise (Emax), 15 (E15) and 60 (E60) min after the test. UA, AA and GRR were increased significantly by the exercise, whereas GSH and Vit A were decreased significantly (P<0.05). Training-related increases (T0 vs. T4 vs. T12) were observed for UA, SOD, GPx and Se, whereas GSH, α-tocopherol and Zn decreased (P<0.05). Exercise intensity (TTF(i) vs. T(i)) increased UA and AA significantly and decreased GSH significantly (P<0.05). A significant correlation between ΔSOD and ΔV˙O2max (r=0.86, p<0.05) determined at SET T4 and SET T12 was found. Taken together, these results indicate that training and exercise intensity significantly influence blood antioxidant markers in healthy Standardbred horses.

Keywords

antioxidant markerstreadmilltrainingexercise intensity
Type
Research Article
Information
Equine and Comparative Exercise Physiology , Volume 1 , Issue 3 , August 2004 , pp. 211 - 220
Copyright
Copyright © Cambridge University Press 2004

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