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Equine osteoarthritis: a brief review of the disease and its causes

Published online by Cambridge University Press:  09 March 2007

Angela E Schlueter  and
Michael W Orth
Angela E Schlueter
Affiliation:
Department of Animal Science, Michigan State University, 2209F Anthony Hall, East Lansing, MI 48824, USA
Michael W Orth*
Affiliation:
Department of Animal Science, Michigan State University, 2209F Anthony Hall, East Lansing, MI 48824, USA
*
* Email:[email protected]
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Abstract

Degenerative joint diseases, such as osteoarthritis, adversely impact the health of the equine athlete as well as the economics of the equine industry. Our understanding of the aetiology of osteoarthritis, although not nearly exhaustive, has increased substantially in recent years. Molecules, including cytokines, inflammatory mediators, and metalloproteinases, have been identified and associated with the progression of joint disease. Several factors, including trauma to the joint, immobilization, conformation, shoeing, and ageing, have been linked with osteoarthritis. Our continued efforts into elucidating critical biological mediators and risk factors, coupled with better chondroprotective therapies and diagnostic tools, should facilitate our ability to maintain the skeletal health of the equine athlete

Keywords

cytokinesmetalloproteinasesjoint diseaserisk factors
Type
Review Article
Information
Equine and Comparative Exercise Physiology , Volume 1 , Issue 4 , November 2004 , pp. 221 - 231
Copyright
Copyright © Cambridge University Press 2004

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References

1Rossdale, PD, Hopes, R, Digby, NJ and Offord, K (1985). Epidemiological study of wastage among racehorses 1982 and 1983. The Veterinary Record 116: 66–69.CrossRefGoogle ScholarPubMed
2Fubini, SL, Erb, HN, Freeman, KP and Todhunter, RJ (1999). Prognostic factors affecting survival of 507 horses with joint disease: (1983 to 1990). Canadian Journal of Veterinary Science 63: 253–260.Google ScholarPubMed
3Jeffcott, LB and Kold, SE (1982). Stifle lameness in the horse: a survey of 86 referred cases. Equine Veterinary Journal 14: 31–39.CrossRefGoogle Scholar
4Todhunter, PG, Kincaid, SA, Todhunter, RJ, Kammermann, JR, Johnstone, B and Baird, AN (1996). Immunohistochemical analysis of an equine model of synovitis-induced arthritis. American Journal of Veterinary Research 57: 1080–1093.CrossRefGoogle ScholarPubMed
5Mackay-Smith, MP (1962). Pathogenesis and pathology of equine osteoarthritis. Journal of Veterinary Medical Science 141: 1246–1252.Google Scholar
6Rose, R (1977). An analysis of conditions causing lameness in the horse. Proceedings of the 54th Annual Meeting of the Australian Veterinary Association 99–102.Google Scholar
7Tew, WP and Hackett, RP (1981). Identification of cartilage wear fragments in synovial fluid from equine joints. Arthritis and Rheumatism 24: 1419–1424.CrossRefGoogle ScholarPubMed
8Cantley, CEL, Firth, EC, Delahunt, JW, Pfeiffer, DU and Thompson, KG (1999). Naturally occurring osteoarthritis in the metacarpophalangeal joints of wild horses. Equine Veterinary Journal 31: 73–81.CrossRefGoogle ScholarPubMed
9Little, CB, Ghosh, P and Rose, R (1997). The effect of strenuous versus moderate exercise on the metabolism of proteoglycans in articular cartilage from different weight-bearing regions of the equine third carpal bone. Osteoarthritis and Cartilage 5: 161–172.CrossRefGoogle Scholar
10Hedbom, E and Hauselmann, HJ (2002). Molecular aspects of pathogenesis in osteoarthritis: the role of inflammation. Cellular and Molecular Life Sciences 59: 45–53.CrossRefGoogle ScholarPubMed
11Bayliss, MT, Hutton, S, Hayward, J and Maciewicz, RA (2001). Distribution of aggrecanase (ADAMts 4/5) cleavage products in normal and osteoarthritic human articular cartilage: the influence of age, topography and zone of tissue. Osteoarthritis and Cartilage 9: 553–560.CrossRefGoogle ScholarPubMed
12Spiers, S, May, SA, Harrison, LJ, Bennett, D and Edwards, GB (1994). Proteolytic enzymes in equine joints with infectious arthritis. Equine Veterinary Journal 26: 48–50.CrossRefGoogle ScholarPubMed
13Spiers, S, May, SA, Bennett, D and Edwards, GB (1994). Cellular sources of proteolytic enzymes in equine joints. Equine Veterinary Journal 26: 43–47.CrossRefGoogle ScholarPubMed
14Morales, TI and Hascall, VC (1988). Correlated metabolism of proteoglycans and hyaluronic acid in bovine cartilage organ cultures. Journal of Biological Chemistry 263: 3632–3638.CrossRefGoogle ScholarPubMed
15Kawcak, CE (2000). Pathophysiology of equine joint disease. Supplement Compendium of Continuing Education Practice for Veterinarians 22: 4–9.Google Scholar
16McIlwraith, CW and Bramlage, LR (1996). Surgical treatment of joint injury. In: McIlwraith, CW and Trotter, (eds) Joint Disease in the Horse. Philadelphia, PA: WB Saunders, p. 490.Google Scholar
17Pond, MJ and Nuki, G (1973). Experimentally induced osteoarthitis in the dog. Annals of Rheumatic Disease 32: 387–388.CrossRefGoogle Scholar
18Moskowitz, RW, Goldberg, VM and Malemud, CJ (1973). Experimentally induced degenerative joint lesions following partial meniscectomy in the rabbit. Arthritis and Rheumatism 16: 397–405.CrossRefGoogle ScholarPubMed
19Troyer, H (1982). Experimental models of osteoarthritis: a review. Seminars in Arthritis and Rheumatism 11: 362–374.CrossRefGoogle ScholarPubMed
20Simmons, EJ, Bertone, AL and Weisbrode, SE (1999). Instability-induced osteoarthritis in the metacarpophalangeal joint of horses. American Journal of Veterinary Research 60: 7–13.CrossRefGoogle ScholarPubMed
21Krause, WR, Pope, MH, Johnson, RJ and Wilder, DG (1976). Mechanical changes in the knee after meniscectomy. Journal of Bone and Joint Surgery American Volume 58: 599–604.Google ScholarPubMed
22Shapiro, F and Glimcher, MJ (1980). Induction of osteoarthrosis in the rabbit knee joint. Clinical Orthopaedics 147: 287–295.CrossRefGoogle Scholar
23Columbo, C, Butler, M, O'Byrne, E, Hickman, L, Swartzendruber, D and Selwyn, M (1983). A new model of osteoarthritis in rabbits. Arthritis and Rheumatism 26: 875–886.CrossRefGoogle Scholar
24McIlwraith, CW and Vachon, A (1988). Review of pathogenesis and treatment of degenerative joint disease. Equine Veterinary Journal Supplement 20: 3–11.CrossRefGoogle Scholar
25Palmer, JL, Bertone, AL and Litsky, AS (1994). Contact area and pressure distribution changes of the equine third carpal bone during loading. Equine Veterinary Journal 26: 197–202.CrossRefGoogle ScholarPubMed
26Morris, EA, McDonald, BS, Webb, AC and Rosenwasser, LJ (1990). Identification of interleukin-1 in equine osteoarthritic joint effusions. American Journal of Veterinary Research 51: 59–64.CrossRefGoogle ScholarPubMed
27Frean, SP, Cambridge, H and Lees, P (2002). Effects of anti-arthritic drugs on proteoglycan synthesis by equine cartilage. Journal of Veterinary Pharmacology and Therapeutics 25: 289–298.CrossRefGoogle ScholarPubMed
28Sadowski, T and Steinmeyer, J (2001). Effects of non-steroidal antiinflammatory drugs and dexamethasone on the activity and expression of matrix metalloproteinase-1, matrix metalloproteinase-3 and tissue inhibitor of metalloproteinases-1 by bovine articular chondrocytes. Osteoarthritis and Cartilage 9: 407–415.CrossRefGoogle ScholarPubMed
29Frisbie, DD, Sandler, EA, Trotter, GW and McIlwraith, CW (2000). Metabolic and mitogenic activities of insulin-like growth factor-1 in interleukin-1-conditioned equine cartilage. American Journal of Veterinary Research 61: 436–441.CrossRefGoogle ScholarPubMed
30Hardy, J, Bertone, AL, Weisbrode, SE, Muir, WW, O'Dorisio, TM and Masty, J (1998). Cell trafficking, mediator release, and articular metabolism in acute inflammation of innervated or denervated isolated equine joints. American Journal of Veterinary Research 59: 88–100.CrossRefGoogle ScholarPubMed
31Platt, D and Bayliss, MT (1994). An investigation of the proteoglycan metabolism of mature equine articular cartilage and its regulation by interleukin-1. Equine Veterinary Journal 26: 297–303.CrossRefGoogle ScholarPubMed
32MacDonald, MH, Stover, SM, Willits, NH and Benton, HP (1992). Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1. American Journal of Veterinary Research 53: 2278–2285.CrossRefGoogle ScholarPubMed
33Morris, EA and Treadwell, BV (1994). Effect of interleukin 1 on articular cartilage from young and aged horses and comparison with metabolism of osteoarthritic cartilage. American Journal of Veterinary Research 55: 138–146.CrossRefGoogle Scholar
34Orth, MW, Peters, TL and Hawkins, JN (2002). Inhibition of articular cartilage degradation by glucosamine-HCl and chondroitin sulphate. Equine Veterinary Journal 34: 224–229.CrossRefGoogle Scholar
35Caron, JP, Fernandes, JC, Martel Pelletier, J, Tardif, G, Mineau, F and Geng, C (1996). Chondroprotective effect of intraarticular injections of interleukin-1 receptor antagonist in experimental osteoarthritis. Suppression of collagenase-1 expression. Arthritis and Rheumatism 39: 1535–1544.CrossRefGoogle ScholarPubMed
36Shinmei, M, Masuda, K, Kikuchi, T and Shimomura, Y (1989). Interleukin 1, tumor necrosis factor, and interleukin 6 as mediators of cartilage destruction. Seminars in Arthritis and Rheumatism 18: 27–32.CrossRefGoogle ScholarPubMed
37Liacini, A, Sylvester, J, Li, WQ, Huang, W, Dehnade, F and Ahmad, M (2003). Induction of matrix metalloproteinase-13 gene expression by TNF-alpha is mediated by MAP kinases, AP-1, and NF-kappaB transcription factors in articular chondrocytes. Experimental Cell Research 288: 208–217.CrossRefGoogle ScholarPubMed
38Flannery, CR, Little, CB, Hughes, CE and Caterson, B (1999). Expression of ADAMTS homologues in articular cartilage. Biochemical and Biophysical Research Communications 260: 318–322.CrossRefGoogle ScholarPubMed
39Richardson, DW and Dodge, GR (2000). Effects of interleukin-1beta and tumor necrosis factor-alpha on expression of matrix-related genes by cultured equine articular chondrocytes. American Journal of Veterinary Research 61: 624–630.CrossRefGoogle ScholarPubMed
40Jouglin, M, Robert, C, Valette, JP, Gavard, F, Quintin-Colonna, F and Denoix, JM (2000). Metalloproteinases and tumor necrosis factor-alpha activities in synovial fluids of horses: correlation with articular cartilage alterations. Veterinary Research 31: 507–515.CrossRefGoogle ScholarPubMed
41Bertone, AL, Palmer, JL and Jones, J (2001). Synovial fluid cytokines and eicosanoids as markers of joint disease in horses. Veterinary Surgery 30: 528–538.CrossRefGoogle ScholarPubMed
42Kallings, P (1993). Nonsteroidal anti-inflammatory drugs. Veterinary Clinics of North America Equine Practice 9: 523–541.CrossRefGoogle ScholarPubMed
43Tung, JT, Venta, PJ, Eberhart, SW, Yuzbasiyan-Gurkan, V, Alexander, L and Caron, JP (2002). Effects of anti-arthritis preparations on gene expression and enzyme activity of cyclooxygenase-2 in cultured equine chondrocytes. American Journal of Veterinary Research 63: 1134–1139.CrossRefGoogle ScholarPubMed
44May, SA, Lees, P, Hooke, RE, Peremans, KY and Verschooten, F (1994). Prostaglandin E2 in equine joint disease. Vlaams Diergeneeskunding Tijdschrift 63: 187–191.Google Scholar
45Moses, VS, Hardy, J, Bertone, AL and Weisbrode, SE (2001). Effects of anti-inflammatory drugs on lipopolysaccharide-challenged and -unchallenged equine synovial explants. American Journal of Veterinary Research 62: 54–60.CrossRefGoogle ScholarPubMed
46MacDonald, MH, Stover, SM, Willits, NH and Benton, HP (1994). Effect of bacterial lipopolysaccharides on sulfated glycosaminoglycan metabolism and prostaglandin E2 synthesis in equine cartilage explant cultures. American Journal of Veterinary Research 55: 1127–1138.CrossRefGoogle ScholarPubMed
47Armstrong, S and Lees, P (1999). Effects of R and S enantiomers and a racemic mixture of carprofen on the production and release of proteoglycan and prostaglandin E2 from equine chondrocytes and cartilage explants. American Journal of Veterinary Research 60: 98–104.CrossRefGoogle Scholar
48von Rechenberg, B, McIlwraith, CW, Akens, MK, Frisbie, DD, Leutenegger, C and Auer, JA (2000). Spontaneous production of nitric oxide (NO), prostaglandin (PGE2) and neutral metalloproteinases (NMPs) in media of explant cultures of equine synovial membrane and articular cartilage from normal and osteoarthritic joints. Equine Veterinary Journal 32: 140–150.CrossRefGoogle Scholar
49Kirker-Head, CA, Chandna, VK, Agarwal, RK, Morris, EA, Tidwell, A, O'Callaghan, MW (2000). Concentrations of substance P and prostaglandin E2 in synovial fluid of normal and abnormal joints of horses. American Journal of Veterinary Research 61: 714–718.CrossRefGoogle ScholarPubMed
50Gibson, KT, Hodge, H and Whittem, T (1996). Inflammatory mediators in equine synovial fluid. Australian Veterinary Journal 73: 148–151.CrossRefGoogle ScholarPubMed
51Kiechle, FL and Malinski, T (1993). Nitric oxide: biochemistry, pathophysiology, and detection. American Journal of Clinical Pathology 100: 567–575.CrossRefGoogle ScholarPubMed
52Arteel, GE, Briviba, K and Sies, H (1999). Protection against peroxynitrite. FEBS Letters 445: 226–230.CrossRefGoogle ScholarPubMed
53Stadler, J, Stefanovic-Racic, M, Billiar, TR, Curran, RD, McIntyre, LA and Georgescu, HI (1991). Articular chondrocytes synthesize nitric oxide in response to cytokines and lipopolysaccharides. Journal of Immunology 147: 3915–3920.CrossRefGoogle Scholar
54Taskiran, D, Stefanovic-Racic, M, Georgescu, H and Evans, C (1994). Nitric oxide mediates suppression of cartilage proteoglycan synthesis by interleukin-1. Biochemical and Biophysical Research Communications 200: 142–148.CrossRefGoogle ScholarPubMed
55Murrell, GAC, Jang, D and Williams, RJ (1995). Nitric oxide activates metalloprotease enzymes in articular cartilage. Biochemical and Biophysical Research Communications 206: 15–21.CrossRefGoogle ScholarPubMed
56Hickery, MS and Bayliss, MT (1998). Interleukin-1 induced nitric oxide inhibits sulphation of glycosaminoglycan chains in human articular chondrocytes. Biochimica et Biophysica Acta 1425: 282–290.CrossRefGoogle ScholarPubMed
57Blanco, FJ, Ochs, RL, Schwarz, H and Lotz, M (1995). Chondrocyte apoptosis induced by nitric oxide. American Journal of Pathology 146: 75–85.Google ScholarPubMed
58Del, Carlo M and Loeser, RF (2002). Nitric oxide-mediated chondrocyte cell death requires the generation of additional reactive oxygen species. Arthritis and Rheumatism 46: 394–403.Google Scholar
59Bird, JL, May, S and Bayliss, MT (2000). Nitric oxide inhibits aggrecan degradation in explant cultures of equine articular cartilage. Equine Veterinary Journal 32: 133–139.CrossRefGoogle ScholarPubMed
60Fermor, B, Haribabu, B, Brice-Weinberg, J, Pisetsky, DS and Guilak, F (2001). Mechanical stress and nitric oxide influence leukotriene production in cartilage. Biochemical and Biophysical Research Communications 285: 806–810.CrossRefGoogle ScholarPubMed
61Pelletier, JP, Jovanovic, DV, Lascau-Coman, V, Fernandes, JC, Manning, PT and Connor, JR (2000). Selective inhibition of inducible nitric oxide synthase reduces progression of experimental osteoarthritis in vivo: possible link with the reduction in chondrocyte apoptosis and caspase 3 level. Arthritis and Rheumatism 43: 1290–1299.3.0.CO;2-R>CrossRefGoogle ScholarPubMed
62Jones, CB, Sane, DC and Herrington, DM (2003). Matrix metalloproteinases: a review of their structure and role in acute coronary syndrome. Cardiovascular Research 59: 812–823.CrossRefGoogle ScholarPubMed
63Brama, PA, TeKoppele, JM, Beekman, B, van Weeren, PR and Barneveld, A (1998). Matrix metalloproteinase activity in equine synovial fluid: influence of age, osteoarthritis, and osteochondrosis. Annals of the Rheumatic Diseases 57: 697–699.CrossRefGoogle ScholarPubMed
64Clegg, PD, Coughlan, AR, Riggs, CM and Carter, SD (1997). Matrix metalloproteinases 2 and 9 in equine synovial fluids. Equine Veterinary Journal 29: 343–348.CrossRefGoogle ScholarPubMed
65Clegg, PD and Carter, SD (1999). Matrix metalloproteinase-2 and -9 are activated in joint diseases. Equine Veterinary Journal 31: 324–330.CrossRefGoogle ScholarPubMed
66Clegg, PD, Radford, AD and Carter, SD (1999). Molecular studies of matrix metalloproteinases and tissue inhibitors of metalloproteinases in equine joints. Veterinary Journal 157: 336–338.CrossRefGoogle ScholarPubMed
67Caron, JP, Tardif, G, Martel-Pelletier, J, DiBattista, JA, Geng, C and Pelletier, JP (1996). Modulation of matrix metalloprotease 13 (collagenase 3) gene expression in equine chondrocytes by interleukin 1 and corticosteroids. American Journal of Veterinary Research 57: 1631–1634.CrossRefGoogle ScholarPubMed
68Billinghurst, RC, Buxton, EM, Edwards, MG, McGraw, MS and McIlwraith, CW (2001). Use of an antineoepitope antibody for identification of type-II collagen degradation in equine articular cartilage. American Journal of Veterinary Research 62: 1031–1039.CrossRefGoogle ScholarPubMed
69Laverty, S, Okouneff, S, Ionescu, M, Reiner, A, Pidoux, I and Webber, C (2002). Excessive degradation of type II collagen in articular cartilage in equine osteochondrosis. Journal of Orthopaedic Research 20: 1282–1289.CrossRefGoogle ScholarPubMed
70Poole, RR (1996). Pathologic manifestations of joint disease in the athletic horse. In McIlwraith, CW and Trotter, GW (eds) Joint Disease in the Horse Philadelphia, PA: WB Saunders. 87–104.Google Scholar
71Moskowitz, RW (2001). Osteoarthritis: simple analgesics versus nonsteroidal antiinflammatory drugs. The Journal of Rheumatology 28: 932–934.Google ScholarPubMed
72Hodgson, DR and Rose, RJ (1994). Principles and Practice of Equine Sports Medicine: The Athletic Horse. Philadelphia, PA: WB Saunders Company, 497.Google Scholar
73Newberry, WN, Mackenzie, CD and Haut, RC (1998). Blunt impact causes changes in bone and cartilage in a regularly exercised animal model. Journal of Orthopedic Research 16: 348–354.CrossRefGoogle Scholar
74Back, W, Schamhardt, HC, Hartman, W and Barneveld, A (1995). Kinematic differences between the distal portions of the forelimbs and hind limbs of horses at the trot. American Journal of Veterinary Research 56: 1522–1528.CrossRefGoogle ScholarPubMed
75McDuffee, LA, Stover, SM and Coleman, K (2000). Limb loading activity of adult horses confined to box stalls in an equine hospital barn. American Journal of Veterinary Research 61: 234–237.CrossRefGoogle Scholar
76Stashak, TS (2002). Examination for lameness. In: Troy, D (ed.) Adams' Lameness in Horses. Philadelphia PA: Williams and Wilkins, pp. 113–183.Google Scholar
77Bowker, RM, Atkinson, PJ, Atkinson, TS and Haut, RC (2001). Effect of contact stress in bones of the distal interphalangeal joint on microscopic changes in articular cartilage and ligaments. American Journal of Veterinary Research 62: 414–424.CrossRefGoogle ScholarPubMed
78Pool, RR and Meagher, DM (1990). Pathologic findings and pathogenesis of racetrack injuries. Veterinary Clinics of North America Equine Practice 6: 1–30.CrossRefGoogle ScholarPubMed
79Brama, PA, Karssenberg, D, Barneveld, A, van Weeren, PR (2001). Contact areas and pressure distribution on the proximal articular surface of the proximal phalanx under sagittal plane loading. Equine Veterinary Journal 33: 26–32.CrossRefGoogle ScholarPubMed
80Palmer, JL, Bertone, AL, Malemud, CJ, Carter, BG, Papay, RS and Mansour, J (1995). Site-specific proteoglycan characteristics of third carpal articular cartilage in exercised and nonexercised horses. American Journal of Veterinary Research 56: 1570–1576.CrossRefGoogle ScholarPubMed
81Murray, RC, Birch, HL, Lakhani, K and Goodship, AE (2001). Biochemical composition of equine carpal articular cartilage is influenced by short-term exercise in a site-specific manner. Osteoarthritis and Cartilage 9: 625–632.CrossRefGoogle Scholar
82Pool, RR (1996). Pathologic manifestations of joint disease in the athletic horse In: McIlwraith, CW and Trotter, GW (eds) Joint Disease in the Horse. Philadelphia, PA: WB Saunders, 87–104.Google Scholar
83Dyson, S (2002). Veterinary review: lameness and poor performance in the sport horse: dressage, show jumping and horse trials. Journal of Equine Veterinary Science 22: 145–150.CrossRefGoogle Scholar
84Ellis, DR and Greenwood, RE (1985). Six cases of degenerative joint disease of the proximal interphalangeal joint of young Thoroughbreds. Equine Veterinary Journal 17: 66–68.CrossRefGoogle ScholarPubMed
85Haakenstad, LH (1968). Chronic bone and joint diseases in relation to conformation in the horse. Equine Veterinary Journal 1: 248–260.CrossRefGoogle Scholar
86Wyn-Jones, G and May, SA (1986). Surgical arthrodesis for the treatment of osteoarthrosis of the proximal intertarsal, distal intertarsal and tarsometatarsal joints in 30 horses; a comparison of four different techniques. Equine Veterinary Journal 18: 59–64.CrossRefGoogle ScholarPubMed
87Adkins, AR, Yovich, JV and Steel, CM (2001). Surgical arthrodesis of distal tarsal joints in 17 horses clinically affected with osteoarthritis. Australian Veterinary Journal 79: 26–29.CrossRefGoogle ScholarPubMed
88Stashak, TS (2002). Lameness. In: Troy, D(ed.) Adams' Lameness in Horses. Philadelphia, PA: Williams and Wilkins 645–1074.Google Scholar
89Eksell, P, Axelsson, M, Brostrom, H, Roneus, B, Haggstrom, J and Carlsten, J (1998). Prevalence and risk factors of bone spavin in Icelandic horses in Sweden: a radiographic field study. Acta Veterinaria Scandinavica 39: 339–348.CrossRefGoogle ScholarPubMed
90Caterson, B and Lowther, DA (1978). Changes in the metabolism of the proteoglycans from sheep articular cartilage in response to mechanical stress. Biochimica et Biophysica Acta 540: 412–422.CrossRefGoogle Scholar
91Richardson, DW and Clark, CC (1993). Effects of short-term cast immobilization on equine articular cartilage. American Journal of Veterinary Research 54: 449–453.CrossRefGoogle ScholarPubMed
92Tammi, M, Saamanen, AM, Jauhiainen, A, Malminen, O, Kiviranta, I and Helminen, H (1983). Proteoglycan alterations in rabbit knee articular cartilage following physical exercise and immobilization. Connective Tissue Research 11: 45–55.CrossRefGoogle ScholarPubMed
93Leroux, MA, Cheung, HS, Bau, JL, Wang, JY, Howell, D and Setton, LA (2001). Altered mechanics and histomorphometry of canine tibial cartilage following joint immobilization. Osteoarthritis and Cartilage 9: 633–640.CrossRefGoogle ScholarPubMed
94Palmoski, MJ and Brandt, KD (1982). Immobilization of the knee prevents osteoarthritis after anterior cruciate ligament transection. Arthritis and Rheumatism 25: 1201–1208.CrossRefGoogle ScholarPubMed
95Auer, JA and Fackelman, GE (1981). Treatment of degenerative joint disease of the horse: a review and commentary. Veterinary Surgery 10: 80–89.CrossRefGoogle Scholar
96Goble, DO (1992). Medical evaluation of the musculoskeletal system and common integument relevant to purchase. Veterinary Clinics of North America Equine Practice 8: 285–302.CrossRefGoogle ScholarPubMed
97Axelsson, M, Bjornsdottir, S, Eksell, P, Haggstrom, J, Sigurdsson, H and Carlsten, J (2001). Risk factors associated with hindlimb lameness and degenerative joint disease in the distal tarsus of Icelandic horses. Equine Veterinary Journal 33: 84–90.CrossRefGoogle ScholarPubMed
98Goodman, NL and Baker, BK (1990). Lameness diagnosis and treatment in the Quarter Horse racehorse. Veterinary Clinics of North America Equine Practice 6: 85–108.CrossRefGoogle ScholarPubMed
99Thompson, KN and Herring, LS (1994). Metacarpophalangeal and phalangeal joint kinematics in horses shod with hoof caulks. Journal of Equine Veterinary Science 14: 319–323.CrossRefGoogle Scholar
100McIlwraith, CW and Goodman, NL (1989). Conditions of the interphalangeal joints. Veterinary Clinics of North America Equine Practice 5: 161–178.CrossRefGoogle ScholarPubMed
101Viitanen, MJ, Wilson, AM, McGuigan, HR, Rogers, KD and May, SA (2003). Effect of foot balance on the intra-articular pressure in the distal interphalangeal joint in vitro. Equine Veterinary Journal 35: 184–189.CrossRefGoogle ScholarPubMed
102Wilson, AM, Seelig, TJ, Shield, RA and Silverman, BW (1998). The effect of foot imbalance on point of force application in the horse. Equine Veterinary Journal 30: 540–545.CrossRefGoogle ScholarPubMed
103Strand, E, Martin, GS, Crawford, MP, Kamerling, SG and Burba, DJ (1998). Intra-articular pressure, elastance and range of motion in healthy and injured racehorse metacarpophalangeal joints. Equine Veterinary Journal 30: 520–527.CrossRefGoogle ScholarPubMed
104McIlwraith, CW (2002). Diseases of joints, tendons, ligaments, and related structures. In: Troy, D (ed) Adams' Lameness in Horses. Philadelphia, PA: Williams and Wilkins, 459–644.Google Scholar
105Brommer, H, van Weeren, PR, Brama, PA and Barneveld, A (2003). Quantification and age-related distribution of articular cartilage degeneration in the equine fetlock joint. Equine Veterinary Journal 35: 697–701.CrossRefGoogle ScholarPubMed
106Brama, PA, TeKoppele, JM, Bank, RA, van Weeren, PR and Barneveld, A (1999). Influence of site and age on biochemical characteristics of the collagen network of equine articular cartilage. American Journal of Veterinary Research 60: 341–345.CrossRefGoogle ScholarPubMed
107Brama, PA Te, Koppele, JM, Bank, RA, Barneveld, A, van, Weeren PR (2002). Development of biochemical heterogeneity of articular cartilage: influence of age and exercise. Equine Veterinary Journal 34: 265–269.CrossRefGoogle ScholarPubMed
108MacDonald, MH, Tesch, AM, Benton, HP and Willits, NH (2002). Characterization of age- and location-associated variation in the composition of articular cartilage from the equine metacarpophalangeal joint. Journal of Equine Veterinary Science 22: 25–32.CrossRefGoogle Scholar
109Vachon, AM, Keeley, FW, McIlwraith, CW and Chapman, P (1990). Biochemical analysis of normal articular cartilage in horses. American Journal of Veterinary Research 51: 1905–1911.CrossRefGoogle ScholarPubMed
110Brown, MP, West, LA, Merritt, KA and Plaas, AH (1998). Changes in sulfation patterns of chondroitin sulfate in equine articular cartilage and synovial fluid in response to ageing and osteoarthritis. American Journal of Veterinary Research 59: 786–791.CrossRefGoogle ScholarPubMed
111Roughley, PJ and White, RJ (1980). Age-related changes in the structure of the proteoglycan subunits from human articular cartilage. Journal of Biological Chemistry 255: 217–224.CrossRefGoogle ScholarPubMed
112Okumura, M, Fujinaga, T, Urakawa, E, Tagami, M and Tsukiyama, K (1997). Evaluation of the catabolic activity of cartilage by measurement of serum keratan sulfate concentration in foals. American Journal of Veterinary Research 58: 925–929.CrossRefGoogle ScholarPubMed
113Thonar, EJ, Pachman, LM, Lenz, ME, Hayford, J, Lynch, P and Kuettner, KE (1988). Age related changes in the concentration of serum keratan sulphate in children. Journal of Clinical Chemistry and Clinical Biochemistry 26: 57–63.Google ScholarPubMed
114Leipold, HR, Goldberg, RL and Lust, G (1989). Canine serum keratan sulfate and hyaluronate concentrations. Relationship to age and osteoarthritis. Arthritis and Rheumatism 32: 312–321.CrossRefGoogle ScholarPubMed
115Todhunter, RJ, Fubini, SL, Freeman, KP and Lust, G (1997). Concentrations of keratan sulfate in plasma and synovial fluid from clinically normal horses and horses with joint disease. Journal of the American Veterinary Medical Association 210: 369–374.CrossRefGoogle ScholarPubMed
116Fuller, CJ, Barr, AR, Dieppe, PA and Sharif, M (1996). Variation of an epitope of keratan sulphate and total glycosaminoglycans in normal equine joints. Equine Veterinary Journal 28: 490–493.CrossRefGoogle ScholarPubMed
117Dean, MF, Lee, YW, Dastjerdi, AM and Lees, P (2003). The effect of link peptide on proteoglycan synthesis in equine articular cartilage. Biochimica et Biophysica Acta 1622: 161–168.CrossRefGoogle ScholarPubMed
118Chubinskaya, S, Kumar, B, Merrihew, C, Heretis, K, Rueger, DC and Kuettner, KE (2002). Age-related changes in cartilage endogenous osteogenic protein-1 (OP-1). Biochimica et Biophysica Acta 1588: 126–134.CrossRefGoogle ScholarPubMed
119Hoekstra, KE, Nielsen, BD, Orth, MW, Rosenstein, DS, Schott, HC and Shelle, JE (1999). Comparison of bone mineral content and biochemical markers of bone metabolism in stall- vs. pasture-reared horses. Equine Veterinary Journal 30: 601–604.CrossRefGoogle Scholar
120Lang, KJ, Nielsen, BD, Waite, KL, Hill, GM and Orth, MW (2001). Supplemental silicon increases plasma and milk silicon concentrations in horses. Journal of Animal Science 79: 2627–2633.CrossRefGoogle ScholarPubMed
121Bell, RA, Nielsen, BD, Waite, K, Rosenstein, D and Orth, M (2001). Daily access to pasture turnout prevents loss of mineral in the third metacarpus of Arabian weanlings. Journal of Animal Science 79: 1142–1150.CrossRefGoogle ScholarPubMed
122Price, JS, Jackson, B, Eastell, R, Goodship, AE, Blumsohn, A and Wright, I (1995). Age related changes in biochemical markers of bone metabolism in horses. Equine Veterinary Journal 27: 201–207.CrossRefGoogle ScholarPubMed