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A whey complement fixation test. Its relation to whey agglutination and isolation of Brucella abortus from the milk of individual cows

Published online by Cambridge University Press:  15 May 2009

I. D. Farrell  and
L. Robertson
I. D. Farrell
Affiliation:
Public Health Laboratory, Preston
L. Robertson
Affiliation:
Public Health Laboratory, Preston
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Summary

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Two hundred and ninety-three milk ring test-positive individual milk samples were examined by whey complement fixation and agglutination tests and the results were compared with the isolation of B. abortus by cultures made from the milk.

There was a closer correlation between whey CF tests and isolation of the organism than between whey AG tests and isolation. Brucella organisms were not isolated from 116 MRT-positive milk samples when the whey CF titre was < 1/10; whereas 9/104 (9%) isolations were made from milk when the whey AG titre was < 1/20.

Because of its closer correlation with culture results it is suggested that the whey CF test would be valuable in an eradication programme for the detection of infected udders.

The limitations of the milk ring test for the examination of individual milk samples are emphasized and it should be used only as a preliminary screening test. In the individual milk samples examined, 32% of the MRT-positives showed no evidence of infection by either the whey AG, whey CF, or culture tests.

Type
Research Article
Information
Journal of Hygiene , Volume 66 , Issue 1 , March 1968 , pp. 19 - 26
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Blake, G. E., Manthei, C. A. & Goode, E. R. (1952). A milk plate test for the detection of brucellosis. J. Am. vet. med. Ass. 120, 1.Google ScholarPubMed
Bradstreet, C. M. P. & Taylor, C. E. D. (1962). Technique of complement-fixation test applicable to the diagnosis of virus diseases. Mon. Bull. Minist. Hlth 21, 96.Google Scholar
Cameron, H. S., Kendrick, J. W. & Merriman, R. W. (1956). A whey-plate test for the diagnosis of bovine brucellosis. J. Am. vet. med. Ass. 129, 19.Google ScholarPubMed
Farrell, I. D. & Robertson, L. (1967). The isolation of Brucella abortus from milk. 1. Biological examination (guinea-pig inoculation). Mon. Bull. Minist. Hlth 26, 52.Google Scholar
Ferguson, G. S. & Robertson, A. (1960). Brucellosis in dairy herds—Some applications of the milk ring test. J. Hyg., Camb. 48, 473.Google Scholar
Fleischhauer, G. (1937). Die Abortus-Bang-Ringprobe (ABR) zur Festellung von bangverdachtigen Vollmilchproben. Berl. tierärztl. Wschr. 53, 527.Google Scholar
Hansard, (1966). Parliamentary Debates (Hansard) House of Commons Official Report. 735, Column 96.Google Scholar
Huddleson, I. F., Hasley, D. E. & Torrey, J. P. (1927). Further studies on the isolation and cultivation of Bacterium abortus. J. infect. Dis. 40, 352.CrossRefGoogle Scholar
Kerr, W. R., Pearson, J. K. L. & Rankin, J. E. F. (1959). The bovine udder and its agglutinins. Br. vet. J. 115, 105.CrossRefGoogle Scholar
Mair, N. S. (1955). A selective medium for the isolation of Brucella abortus from herd samples of milk. Mon. Bull. Minist. Hlth 14, 184.Google Scholar
Ministry of Health Circular 17/66. 24 Oct. 1966.Google Scholar
Morris, E. (1956). A selective medium for Brucella spp. J. gen. Microbiol. 15, 629.CrossRefGoogle ScholarPubMed
Report (1956). The value of the Milk Ring Test (M.R.T.) and related tests in the detection of brucella organisms in milk supplies. Mon. Bull. Minist. Hlth 15, 85.Google Scholar
Report (1964 a). Tech. Rep. Ser. Wld Hlth Org. no. 289.Google Scholar
Report (1964 b). Brucellosis in the British Dairy Herd. Animal Disease Survey, no. 4. H.M.S.O.Google Scholar
Robertson, L. (1967). Diagnosis and treatment of infection with Brucella abortus, biotype 5. J. clin. Path. 20, 199.CrossRefGoogle ScholarPubMed
Smith, T., Orcutt, M. L. & Little, R. B. (1923). The source of agglutinins in the milk of cows. J. exp. Med. 37, 153.CrossRefGoogle ScholarPubMed
Traum, J. & Maderious, W. E. (1947). The interpretation of whey agglutination test results in cows vaccinated with Brucella abortus strain 19. Am. J. vet. Res. 8, 244.Google ScholarPubMed