Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-22T16:01:35.202Z Has data issue: false hasContentIssue false
  • English
  • Français

Virulence properties of Escherichia coli strains belonging to serogroups O26, O55, O111 and O128 isolated in the United Kingdom in 1991 from patients with diarrhoea

Published online by Cambridge University Press:  15 May 2009

S. M. Scotland ,
G. A. Willshaw ,
H. R. Smith ,
B. Said ,
N. Stokes  and
B. Rowe
S. M. Scotland
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
G. A. Willshaw
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
H. R. Smith
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
B. Said
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
N. Stokes
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Some strains of Escherichia coli belonging to serogroups O26, O55, O111 or O128 produce Vero cytotoxin (VT). These serogroups are included in the range of enteropathogenic E. coli (EPEC) serogroups for which commercial antisera are available. In an attempt to obtain information on VT-producing strains other than those of serogroup O157, 122 strains belonging to these four serogroups and isolated in 1991 from patients with diarrhoea in the United Kingdom were tested for hybridization with VT probes. Only 18 of the 122 strains were VT-positive and these were O26 or O128. However 90 strains hybridized with the E. coli attaching and effacing (eae) probe (including 14 VT-positive strains) and 17 with the enteroaggregative E. coli (EAggEC) probe. For 78 eae-positive and 9 EAggEC-positive strains, tissue culture tests correlated with the probe results as the strains gave, respectively, either localized adhesion and a positive fluorescent-actin staining test or a characteristic aggregative attachment. A total of 111 of the 122 strains belonging to serogroups O26, O55, O111 or O128 possessed properties that may be associated with the ability to cause human diarrhoeal disease, and similar studies are needed on strains from the other classical EPEC serogroups.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 3 , December 1993 , pp. 429 - 438
Copyright
Copyright © Cambridge University Press 1993

References

1.Karmali, MA. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 1989; 2: 15–38.CrossRefGoogle ScholarPubMed
2.Smith, HR, Scotland, SM. Vero cytotoxin-producing strains of Escherichia coli. J Med Microbiol 1988; 26: 77–85.CrossRefGoogle ScholarPubMed
3.Riley, LW. The epidemiologic, clinical, and microbiologic features of hemorrhagic colitis. Ann Rev Microbiol 1987; 41: 383–407.CrossRefGoogle ScholarPubMed
4.Griffin, PM, Tauxe, RV. The epidemiology of infections caused by Escherichia coli O157: H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev 1991; 13: 60–98.CrossRefGoogle ScholarPubMed
5.Bitzan, M, Karch, H, Maas, MG, et al. Clinical and genetic aspects of shiga-like toxin production in traditional enteropathogenic Escherichia coli. Zentralbl Bakteriol 1991; 274: 496–506.CrossRefGoogle ScholarPubMed
6.Bopp, CA, Greene, KD, Downes, FP, Sowers, EG, Wells, JG, Wachsmuth, IK. Unusual verotoxin-producing Escherichia coli associated with hemorrhagic colitis. J Clin Microbiol 1987; 25: 1486–9.CrossRefGoogle ScholarPubMed
7.Katouli, M, Kühn, I, Möllby, R. Biochemical phenotypes of enteropathogenic Escherichia coli common to Iran and Sweden. J Med Microbiol 1991; 35: 270–7.CrossRefGoogle ScholarPubMed
8.Konowalchuk, J, Speirs, JI, Stavric, S. Vero response to a cytotoxin of Escherichia coli. Infect Immun 1977; 18: 775–9.CrossRefGoogle ScholarPubMed
9.Scotland, SM, Day, NP, Rowe, B. Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microbiol Lett 1980; 7: 15–7.CrossRefGoogle Scholar
10.Scotland, SM, Willshaw, GA, Smith, HR, Rowe, B. Properties of strains of Escherichia coli O26: H11 in relation to their enteropathogenic or enterohemorrhagic classification. J Infect Dis 1990; 162: 1069–74.CrossRefGoogle ScholarPubMed
11.Willshaw, GA, Scotland, SM, Smith, HR, Rowe, B. Properties of Vero cytotoxin-producing Escherichia coli of human origin belonging to O-serogroups other than O157. J Infect Dis 1992; 166: 797–802.CrossRefGoogle Scholar
12.Gross, RJ, Rowe, B. Serotyping of Escherichia coli. In: Sussman, M, ed. The virulence of Escherichia coli: reviews and methods. London: Academic Press, 1985: 345–63.Google Scholar
13.Willshaw, GA, Smith, HR, Scotland, SM, Field, AM, Rowe, B. Heterogeneity of Escherichia coli phages encoding Vero cytotoxins: comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. J Gen Microbiol 1987; 133: 1309–17.Google ScholarPubMed
14.Thomas, A, Smith, HR, Willshaw, GA, Rowe, B. Non-radioactively labelled polynucleotide and oligonucleotide DNA probes, for selectively detecting Escherichia coli strains producing Vero cytotoxins VT1, VT2 and VT2 variant. Molec Cell Probes 1991; 5: 129–35.CrossRefGoogle ScholarPubMed
15.Levine, MM, Xu, J-g, Kaper, JB, et al. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis 1987; 156: 175–82.CrossRefGoogle ScholarPubMed
16.Baldini, MM, Nataro, JP, Kaper, JB. Localization of a determinant for HEp-2 adherence by enteropathogenic Escherichia coli. Infect Immun 1986; 52: 334–6.CrossRefGoogle ScholarPubMed
17.Jerse, AE, Yu, J, Tall, BD, Kaper, JB. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 1990; 87: 7839–43.CrossRefGoogle ScholarPubMed
18.Baudry, B, Savarino, SJ, Vial, P, Kaper, JB, Levine, MM. A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. J Infect Dis 1990; 161: 1249–51.CrossRefGoogle ScholarPubMed
19.Bilge, SS, Clausen, CR, Lau, W, Moseley, SL. Molecular characterization of a fimbrial adhesin, F1845. mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J Bacteriol 1989; 171: 4281–9.CrossRefGoogle ScholarPubMed
20.Maniatis, T, Fritsch, EF, Sambrook, J. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1982.Google Scholar
21.Moseley, SL, Hardy, JW, Huq, MI, Echeverria, P, Falkow, S. Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli. Infect Immun 1983; 39: 1167–74.CrossRefGoogle ScholarPubMed
22.Scotland, SM, Willshaw, GA, Smith, HR, Gross, RJ, Rowe, B. Adhesion to cells in culture and plasmid profiles of enteropathogenic Escherichia coli isolated from outbreaks and sporadic cases of infant diarrhoea. J Infect 1989; 19: 237–49.CrossRefGoogle ScholarPubMed
23.Knutton, S, Baldwin, T, Williams, PH, McNeish, AS. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun 1989; 57: 1290–8.CrossRefGoogle ScholarPubMed
24.Willshaw, GA, Smith, HR, McConnell, MM, et al. Plasmid-encoded production of coli-surface associated antigen 1 (CS1) in a strain of Escherichia coli serotype O139. H28. Microb Path 1990; 9: 1–11.CrossRefGoogle Scholar
25.Beutin, L, Montenegro, MA, Ørskov, I, et al. Close association of verotoxin (Shiga-like toxin) production with enterohaemolysin production in strains of Escherichia coli. J Clin Microbiol 1989; 27: 2559–64.CrossRefGoogle Scholar
26.Levine, MM, Prado, V, Kaper, JB, et al. DNA probes to detect diarrheagenic Escherichia coli. In: Balows, A, Tilton, RC, Turano, A, eds. Rapid methods and automation in microbiology and immunology. Brescia, Italy: Brixia Academic Press, 1989: 420–7.Google Scholar
27.Beutin, L, Bode, L, Ozel, M, Stephan, R. Enterohemolysin production is associated with a temperate bacteriophage in Escherichia coli serogroup O26 strains. J Bacteriol 1990; 172: 6469–75.CrossRefGoogle ScholarPubMed
28.Jerse, AE, Kaper, JB. The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect Immun 1991; 59: 4302–9.CrossRefGoogle ScholarPubMed
29.Moyenuddin, M, Wachsmuth, IK, Moseley, SL, Bopp, CA, Blake, PA. Serotype, antimicrobial resistance, and adherence properties of Escherichia coli strains associated with outbreaks of diarrheal illness in children in the United States. J Clin Microbiol 1989; 27: 2234–9.CrossRefGoogle ScholarPubMed
30.Knutton, S, Lloyd, DR, McNeish, AS. Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infect Immun 1987; 55: 69–77.CrossRefGoogle ScholarPubMed
31.Rothbaum, R, McAdams, AJ, Giannella, R, Partin, JC. A clinicopathologic study of enterocyte-adherent Escherichia coli: a cause of protracted diarrhea in infants. Gastroenterology 1982; 83: 441–54.CrossRefGoogle ScholarPubMed
32.Tzipori, S, Gibson, R, Montanaro, J. Nature and distribution of mucosal lesions associated with enteropathogenic and enterohemorrhagic Escherichia coli in piglets and the role of plasmid-mediated factors. Infect Immun 1989; 57: 1142–50.CrossRefGoogle ScholarPubMed
33.Smith, HR, Scotland, SM, Stokes, N, Rowe, B. Examination of strains belonging to enteropathogenic Escherichia coli serogroups for genes encoding EPEC adherence factor and Vero cytotoxins. J Med Microbiol 1990; 31: 235–40.CrossRefGoogle ScholarPubMed
34.Scotland, SM, Smith, HR, Rowe, B. Escherichia coli O128 strains from infants with diarrhea commonly show localized adhesion and positivity in the fluorescent-actin staining test but do not hybridize with an enteropathogenic E. coli adherence factor probe. Infect Immun 1991; 59: 1569–71.CrossRefGoogle Scholar
35.Scotland, SM, Smith, HR, Said, B, Willshaw, GA, Cheasty, T, Rowe, B. Identification of enteropathogenic Escherichia coli isolated in Britain as enteroaggregative or as members of a subclass of attaching-and-effacing E. coli not hybridising with the EPEC adherence-factor probe. J Med Microbiol 1991; 35: 278–83.CrossRefGoogle Scholar
36.Knutton, S, Phillips, AD, Smith, HR, et al. Screening for enteropathogenic Escherichia coli in infants with diarrhea by the fluorescent-actin staining test. Infect Immun 1991; 59: 365–71.CrossRefGoogle ScholarPubMed
37.Levine, MM, Bergquist, EJ, Nalin, DR, et al. Escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet 1978; i: 1119–22.CrossRefGoogle Scholar
38.Bhan, MK, Raj, P, Levine, MM, et al. Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India. J Infect Dis 1989; 159: 1061–4.CrossRefGoogle Scholar
39.Cravioto, A, Tello, A, Navarro, A, et al. Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea. Lancet 1991; 337: 262–4.CrossRefGoogle ScholarPubMed
40.Cantey, JR, Moseley, SL. HeLa cell adherence, actin aggregation, and invasion by nonenteropathogenic Escherichia coli possessing the eae gene. Infect Immun 1991; 59: 3924–9.CrossRefGoogle ScholarPubMed
41.Morris, KJ, Rao, GG. Conventional screening for enteropathogenic Escherichia coli in the UK. Is it appropriate or necessary? J Hosp Infect 1992; 21: 163–7.CrossRefGoogle ScholarPubMed