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Virulence determinants in Pseudomonas aeruginosa strains from urinary tract infections

Published online by Cambridge University Press:  15 May 2009

P. Visca ,
F. Chiarini ,
A. Mansi ,
C. Vetriani ,
L. Serino  and
N. Orsi
P. Visca
Affiliation:
Institute of Microbiology, Faculty of Medicine, University of Rome ‘La Sapienza’, P. le A. Moro, 5, 00185 Rome, Italy
F. Chiarini
Affiliation:
Institute of Microbiology, Faculty of Medicine, University of Rome ‘La Sapienza’, P. le A. Moro, 5, 00185 Rome, Italy
A. Mansi
Affiliation:
Institute of Microbiology, Faculty of Medicine, University of Rome ‘La Sapienza’, P. le A. Moro, 5, 00185 Rome, Italy
C. Vetriani
Affiliation:
Institute of Microbiology, Faculty of Medicine, University of Rome ‘La Sapienza’, P. le A. Moro, 5, 00185 Rome, Italy
L. Serino
Affiliation:
Institute of Microbiology, Faculty of Medicine, University of Rome ‘La Sapienza’, P. le A. Moro, 5, 00185 Rome, Italy
N. Orsi
Affiliation:
Institute of Microbiology, Faculty of Medicine, University of Rome ‘La Sapienza’, P. le A. Moro, 5, 00185 Rome, Italy
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A total of 121 uropathogenic Pseudomonas aeruginosa strains were examined for production of several virulence-related factors. These strains were distributed in five predominant O-serotypes, i.e. O 4, O 12, O 11, O 6 and O 5, which accounted respectively for 23·9, 23·1, 12·3, 8·2 and 5·7% of isolates. Pyochelin and pyoverdin siderophores were produced by most of the isolates, defective variants occurring at very low frequency (2·4% for pyochelin and 7·4% for pyoverdin). Adherence to uroepithelial cells and production of cytotoxins was demonstrated in 52·8 and 67·7% of the strains, respectively, with higher frequencies for epidemiologically related strains belonging to serotypes O 4 and O 12. Titration of total proteases, elastase and phospholipase C revealed a high degree of heterogeneity among isolates. However, examination of individual O-serotypes by exoenzyme production showed that elevated levels of total proteases and elastase were characteristics of serotypes of minor numerical importance, i.e. O 1, O 10, O 11 and O 17, whilst low levels of elastase were produced by strains belonging to the predominant serotypes, namely O 4 and O 12. Moreover, epidemiologically related strains belonging to serotypes O 4 and O 12 appeared more homogeneous than the whole serogroup, when compared with other groups on the basis of exoenzyme levels.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 2 , April 1992 , pp. 323 - 336
Copyright
Copyright © Cambridge University Press 1992

References

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