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Variability in the characteristics of pocks produced on the chick chorioallantois by white pock mutants of cowpox and other poxviruses

Published online by Cambridge University Press:  15 May 2009

Derrick Baxby
Derrick Baxby
Affiliation:
Department of Bacteriology, Liverpool University, P.O.Box 147, Liverpool, England
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White pock mutants of cowpox virus produce pocks in the CAM which ‘mimic’ those produced by the parent virus, in macroscopic and microscopic appearance, and in the amount of virus they contain. The proportion of such ‘atypical’ pocks was influenced by the age and source of embryo, incubation temperature and position of the CAM during incubation. Similar results were obtained with white pock mutants isolated from strains of vaccinia, rabbitpox and monkeypox. The appearance of pocks produced by variola, alastrim, ectromelia and vaccinia (Lister) was unaffected by changes in environmental conditions.

I would like to thank Professor K. McCarthy, Professor K. R. Dumbell, Dr C. H. Taylor-Robison and Dr C. R. Madeley for their interest and for kindly providing virus strains, and Miss M. Seyfang for technical assistance.

I would like to thank the Committee on Research, Liverpool University, for a grant towards the cost of the colour plate.

Type
Research Article
Information
Journal of Hygiene , Volume 67 , Issue 4 , December 1969 , pp. 637 - 647
Copyright
Copyright © Cambridge University Press 1969

References

Baxby, D. & Rondle, C. J. M. (1967). The relative sensitivity of chick and rabbit tissues for the titration of vaccinia and cowpox viruses. Arch ges. Virusforsch. 20, 263.CrossRefGoogle ScholarPubMed
Baxby, D. & Rondle, C. J. M. (1968). The inhibition of growth of vaccinia and cowpox viruses in RK 13 cells. J. Hyg., Camb. 66, 191.CrossRefGoogle ScholarPubMed
Bedson, H. S. & Dumbell, K. R. (1964). Hybrids derived from the viruses of variola major and cowpox. J. Hyg., Camb. 62, 147.CrossRefGoogle ScholarPubMed
Downie, A. W. (1939). A study of the lesions produced experimentally by cowpox virus. J. Path. Bact. 48, 361.CrossRefGoogle Scholar
Downie, A. W. & Haddock, D. R. W. (1952). A variant of cowpox. Lancet, i, 1049.CrossRefGoogle Scholar
Dumbell, K. R., Bedson, H. S. & Rossier, E. (1961). The laboratory differentiation between variola major and variola minor. Bull. Wld Hlth Org. 25, 73.Google ScholarPubMed
Fenner, F. (1958). The biological characteristics of several strains of vaccina, cowpox and rabbitpox viruses. Virology 5, 502.CrossRefGoogle Scholar
Fenner, F. & Comben, B. M. (1958). Genetic studies with mammalian poxviruses. 1. Demonstration of recombination between two strains of vaccina virus. Virology 5, 530.CrossRefGoogle Scholar
Fenner, F. & Greenland, R. M. (1964). Unpublished work cited in Fenner, F. (1965). Conditional lethal mutants in the study of the genetics of animal virus. Perspectives in Virology 4, 34.Google Scholar
Fenner, F. & Woodroofe, G. M. (1960). The reactivation of poxviruses. II. The range of reactivating viruses. Virology 11, 185.CrossRefGoogle ScholarPubMed
Haddock, D. R. W. (1952). Variation in viruses, M.D. thesis, Liverpool University.Google Scholar
McCarthy, K. & Dumbell, K. R. (1961). Chorioallantoic inoculation of eggs, an improved method. Virology 14, 488.CrossRefGoogle ScholarPubMed
McClain, M. E. (1965). The host range and plaque morphology of rabbitpox virus (RPu+) and its u mutants on chick fibroblast, PK-2a, and L 929 cells. Aust. J. exp. Biol. med. Sci. 43, 31.CrossRefGoogle Scholar
Rondle, C. J. M. & Dumbell, K. R. (1962). Antigens of cowpox virus. J. Hyg., Camb. 60, 41.CrossRefGoogle ScholarPubMed
Westwood, J. C. N., Phipps, P. H. & Boulter, E. A. (1957). The titration of vaccinia virus on the chorioallantoic membrane of the developing chick embryo. J. Hyg., Camb. 55, 123.CrossRefGoogle ScholarPubMed
Woodroofe, G. M. & Fenner, F. (1960). Genetic studies with mammalian poxviruses. IV. Hybridization between several different poxviruses. Virology 12, 272.CrossRefGoogle ScholarPubMed