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The use of single-radial-haemolysis for rubella antibody studies

Published online by Cambridge University Press:  15 May 2009

Mairin Clarke ,
Janet Boustred ,
Valerie Seagroatt  and
G. C. Schild
Mairin Clarke
Affiliation:
Division of Viral Products, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
Janet Boustred
Affiliation:
Division of Viral Products, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
Valerie Seagroatt
Affiliation:
Division of Viral Products, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
G. C. Schild
Affiliation:
Division of Viral Products, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
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The use of a single-radial-haemolysis technique for the detection of antibody to rubella virus is described. The single-radial-haemolysis test was compared with the standard HI methods for the detection of antibody to rubella virus. A close correlation between the two methods was observed in a survey of over two thousand serum samples and the study indicated that single-radial-haemolysis was highly satisfactory as an assay method for IgG antibodies to rubella virus. It was found that the immuno-globulins active in SRH tests sedimented in the 7S range in sucrose rate gradients and were presumably immunoglobulins of the IgG class, but 19S iinmunoglobulins did not produce haemolysis.

Type
Research Article
Information
Journal of Hygiene , Volume 79 , Issue 3 , December 1977 , pp. 355 - 364
Copyright
Copyright © Cambridge University Press 1977

References

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