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Use of single radial immunodiffusion test for serological studies in volunteers inoculated with live attenuated influenza virus

Published online by Cambridge University Press:  15 May 2009

M. E. Molyneux ,
A. S. Beare ,
K. Callow  and
G. C. Schild
M. E. Molyneux
Affiliation:
Clinical Research Centre, Harrow, Middlesex, and Common Cold Unit, Salisbury, Wilts.
A. S. Beare
Affiliation:
Clinical Research Centre, Harrow, Middlesex, and Common Cold Unit, Salisbury, Wilts.
K. Callow
Affiliation:
Clinical Research Centre, Harrow, Middlesex, and Common Cold Unit, Salisbury, Wilts.
G. C. Schild
Affiliation:
National Institute for Medical Research, Mill Hill, NW 7 1AA
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Summary

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Pre- and post-vaccination serum samples from 278 volunteers, who were given live influenza vaccines, were tested by haemagglutination inhibition (HI) and single radial immunodiffusion tests (SRDT) for antibody to influenza A/Hong Kong/1/68 (H3N2) virus envelope antigens. Those with high antibody titres detected in both tests were less frequently infected, and 85% of the 159 infected showed rises by HI and 70% by SRDT. Similarly, 69 pairs were tested for antibody to Hong Kong (N2) neuraminidase by neuraminidase inhibition test (NI) and by SRD tests. Those with high titres in both tests resisted infection and those who were infected showed a rise in antibody detected both by NI and SRD tests. In general, SRDT was less sensitive than HI and NI in detecting antibody and antibody rises, but in some volunteers it did detect antibody rises which were not detected by conventional tests. Because of its simplicity and speed it appeared to be of use in evaluating such vaccines.

Type
Research Article
Information
Journal of Hygiene , Volume 73 , Issue 2 , October 1974 , pp. 287 - 294
Copyright
Copyright © Cambridge University Press 1974

References

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