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Use of AFLP, plasmid typing and phenotyping in a comparative study to assess genetic diversity of Shigella flexneri strains

Published online by Cambridge University Press:  10 January 2003

S. HERRERA ,
R. CABRERA ,
M. M. RAMIREZ ,
M. A. USERA  and
M. A. ECHEITA
S. HERRERA
Affiliation:
Laboratorio Nacional de Referencia de Salmonella y Shigella, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III
R. CABRERA
Affiliation:
Laboratorio Nacional de Referencia de Salmonella y Shigella, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III Laboratorio Nacional de Referencia de Enfermedades Diarreicas Agudas, Instituto de Medicina Tropical ‘Pedro Kourí’Cuba
M. M. RAMIREZ
Affiliation:
Laboratorio Nacional de Referencia de Salmonella y Shigella, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III Laboratorio Nacional de Referencia de Enfermedades Diarreicas Agudas, Instituto de Medicina Tropical ‘Pedro Kourí’Cuba
M. A. USERA
Affiliation:
Laboratorio Nacional de Referencia de Salmonella y Shigella, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III
M. A. ECHEITA
Affiliation:
Laboratorio Nacional de Referencia de Salmonella y Shigella, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III
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Abstract

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Shigella flexneri infections are one of the main causes of acute diarrhoea in Cuba. Twenty strains isolated from sporadic cases in nine different Cuban provinces were characterized. Serotyping, antibiotic-resistance typing, plasmid-typing and AFLP-typing were used to determine their suitability for use in epidemiological studies of S. flexneri. The predominant serotypes were serotype 6 (35%) and serotype 2 (35%). Eleven different plasmid profiles were detected (Diversity Index=0·92). AFLP-typing discriminated 12 different patterns (DI=0·95), these patterns were not coincident with plasmid-typing patterns. Both techniques combined distinguished 14 patterns among the 20 studied strains (DI=0·99). There was no consistent relationship between plasmid-typing and AFLP-typing patterns or antibiotic-resistance typing patterns. Ninety-five percent of S. flexneri strains were multiresistant.

Type
Research Article
Information
Epidemiology & Infection , Volume 129 , Issue 3 , December 2002 , pp. 445 - 450
Copyright
2002 Cambridge University Press