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Urinary isolates of apramycin-resistant Escherichia coli and Klebsiella pneumoniae from Dublin

Published online by Cambridge University Press:  15 May 2009

A. P. Johnson ,
M. Malde ,
N. Woodford ,
R. J. Cunney  and
E. G. Smyth
A. P. Johnson
Affiliation:
Antibiotic Reference Unit, Laboratory of Hospital Infection, Central Public Health Laboratory, London NW9 5HT
M. Malde
Affiliation:
Antibiotic Reference Unit, Laboratory of Hospital Infection, Central Public Health Laboratory, London NW9 5HT
N. Woodford
Affiliation:
Antibiotic Reference Unit, Laboratory of Hospital Infection, Central Public Health Laboratory, London NW9 5HT
R. J. Cunney
Affiliation:
Department of Clinical Microbiology, Beaumont Hospital, Dublin 9
E. G. Smyth
Affiliation:
Department of Clinical Microbiology, Beaumont Hospital, Dublin 9
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Summary

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Twenty-two gentamicin-resistant urinary isolates of Escherichia coli and five gentamicin-resistant urinary isolates of Klebsiella pneumoniae from a Dublin hospital were examined for resistance to the veterinary aminoglycoside antibiotic apramycin. Five isolates of E. coli and one isolate of K. pneumoniae were found to be resistant. The apramycin-resistant isolates, which were also resistant to the veterinary anthelmintic agent hygromycin B, hybridized with a DNA probe for the gene encoding the enzyme 3-N-aminoglycoside acetyltransferase type IV (AAC(3)IV). Resistance to apramycin and hygromycin B was co-transferable in four of the five isolates of E. coli and the isolate of K. pneumoniae. In one isolate of E. coli apramycin resistance was not transferable. On the basis of their restriction enzyme digestion profiles and the antimicrobial resistance traits encoded, the transferable plasmids encoding resistance to apramycin and hygromycin B comprised three distinct types. Genetic linkage between the gene encoding AAC(3)IV and genes encoding resistance to ampicillin and either tetracycline or trimethoprim, means that the relatively widespread use of these antimicrobial agents provides a selective pressure for the persistence of resistance to apramycin and gentamicin even in the absence of bacterial exposure to aminoglycosides.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 105 - 112
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Wray, C.Hedges, RW.Shannon, KP, Bradley, DE. Apramycin and gentamicin resistance in Escherichia coli and salmonellas isolated from farm animals. J Hyg 1986; 97: 445–56.CrossRefGoogle ScholarPubMed
2.Wick, WE.Welles, JS. Nebramycin. a new broad-spectrum antibiotic complex. In: Hobby, GL, ed. Antimicrobial agents and chemotherapy – 1967. Ann Arbor Michigan: American Society for Microbiology, 1968: 341–8.Google Scholar
3.Ryden, R, Moore, BJ. The in vitro activity of apramycin, a new aminocyclitol antibiotic. J Antimicrob Chemother 1977; 3: 609–13.CrossRefGoogle ScholarPubMed
4.Threlfall, EJ.Rowe, B.Ferguson, JL.Ward, LR. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg 1986; 97: 419–26.Google Scholar
5.Hunter, JEB.Shelley, JC, Walton, JR, Hart, CA, Bennett, M. Apramycin resistance plasmids in Escherichia coli: Possible transfer to Salmonella typhimurium in calves. Epidemiol Infect 1992; 108: 271–8.Google Scholar
6.Chaslus-Dancla, E, Martel, J-L, Carlier, C, Lafont, JP, Courvalin, P. Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France. Antimicrob Agents Chemother 1986; 29: 239–43.CrossRefGoogle ScholarPubMed
7.Davies, J.O'Connor, S. Enzymatic modification of aminoglycoside antibiotics: 3-N-aminoacetyltransferase with broad specificity that determines resistance to the novel aminoglycoside apramycin. Antimicrob Agents Chemother 1978; 14: 69–72.CrossRefGoogle Scholar
8.Chaslus-Dancla, E, Glupczynski, Y, Gerbaud, G, Lagorce, M, Lafont, JP, Courvalin, P. Detection of apramycin-resistant Enterobacteriaceae in hospital isolates. FEMS Microbiol Lett 1989; 61: 261–6.CrossRefGoogle Scholar
9.Salauze, D, Otal, I, Gomez-Lus, R, Davies, J. Aminoglycoside acetyltransferase 3-IV (aacC4) and hygromycin B 4–1 phosphotransferase (hyphB) in bacteria isolated from human and animal sources. Antimicrob Agents Chemother 1990; 34: 1915–20.CrossRefGoogle Scholar
10.Chaslus-Dancla, E, Pohl, P, Meurisse, M, Marin, M, Lafont, JP. High genetic homology between plasmids of human and animal origins conferring resistance to the aminoglycosides gentamicin and apramycin. Antimicrob Agents Chemother 1991; 35: 590–3.CrossRefGoogle Scholar
11.Hunter, JEB, Hart, CA, Shelley, JC, Walton, JR, Bennett, M. Human isolates of apramycin- resistant Escherichia coli which contain the genes for the AAC(3)IV enzyme. Epidemiol Infect 1993; 110: 253–9.Google Scholar
12.Pohl, P, Glupczynski, Y, Marin, M, Van Robaeys, G, Lintermans, P, Couturier, M. Replicon typing characterization of plasmids encoding resistance to gentamicin and apramycin in Escherichia coli and Salmonella typhimurium isolated from human and animal sources in Belgium. Epidemiol Infect 1993; 111: 229–38.Google Scholar
13.Johnson, AP, Burns, L, Woodford, N et al. Gentamicin resistance in clinical isolates of Escherichia coli encoded by genes of veterinary origin. J Med Microbiol 1994; 40: 221–6.Google Scholar
14.Hunter, JEB, Bennett, M, Hart, CA, Shelley, JC, Walton, JR. Apramycin-resistant E. coli isolated from pigs and a stockman. Epidemiol Infect 1994: 112: 473–80.CrossRefGoogle ScholarPubMed
15.Working Party on Antibiotic Sensitivity Testing of the British Society for Antimicrobial Chemotherapy. A guide to sensitivity testing. J Antimicrob Chemother 1991; 27 Suppl D: 7–9.Google Scholar
16.Kado, CI, Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–73.CrossRefGoogle ScholarPubMed
17.Meynell, E, Datta, N. The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli K12. Genet Res 1966; 7: 134–40.CrossRefGoogle Scholar
18.Matthew, M, Harris, AM, Marshall, MJ, Ross, GW. The use of analytical isoelectric focusing for detection and identification of β-lactamases. J Gen Microbiol 1975; 88: 169–78.CrossRefGoogle Scholar