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The typing of enteroviruses in tissue culture by neutralization with composite antiserum pools

Published online by Cambridge University Press:  15 May 2009

M. H. Hambling ,
Patricia M. Davis  and
A. D. Macrae
M. H. Hambling
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory, Colindale, London, N. W. 9
Patricia M. Davis
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory, Colindale, London, N. W. 9
A. D. Macrae
Affiliation:
Virus Reference Laboratory, Central Public Health Laboratory, Colindale, London, N. W. 9
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A scheme is described for the identification of enteroviruses in tissue culture by neutralization with composite antiserum pools. The method for making these pools is given. Antisera to twenty-seven enteroviruses were included in the pools which were used to examine 115 viruses consisting of ninety-eight recently isolated viruses and seventeen prototype strains. The results indicate that this scheme provides a useful screening method for identifying enteroviruses. It has proved to be practicable, time saving and very economical in tissue culture.

We are grateful to Dr C. M. P. Bradstreet who made available some of the rabbit antisera.

Type
Research Article
Information
Journal of Hygiene , Volume 61 , Issue 4 , December 1963 , pp. 479 - 484
Copyright
Copyright © Cambridge University Press 1963

References

Karber, G. (1931). Beitrag zur kollektiven Bhandlung pharmakologischer Reihenversuche. Arch. exp. Path. Pharmaic. 162, 480.CrossRefGoogle Scholar
Lim, K. A. & Benyesh-Melnick, M. (1960). Typing of viruses by combination of antiserum pools. Application to typing of enteroviruses (Coxsackie and ECHO). J. Immunol. 84, 309.CrossRefGoogle Scholar
Melnick, J. L. (1958). Advances in the study of enteroviruses. Proyr. med. Virol. 1, 59.Google Scholar
Report (1962). Classification of human enteroviruses. Virology, 16, 501.CrossRefGoogle Scholar
Schmidt, N. J., Guenther, R. W. & Lennette, E. H. (1961). Typing of ECHO virus isolates by immune serum pools. The ‘intersecting serum scheme’;. J. Immunol. 87, 623.CrossRefGoogle ScholarPubMed