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Trimethoprim-resistance and its transferability in E. coli isolated from calves treated with trimethoprim-sulphadiazine: a two year study

Published online by Cambridge University Press:  15 May 2009

M. P. Fleming
M. P. Fleming
Affiliation:
Wellcome Veterinary Research Station, Frant, Tunbridge Wells, Kent*
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Regular examination of rectal swabs revealed the presence of very low numbers of trimethoprim resistant Escherichia coli in the faeces of 10 batches of calves successively reared in the same shed and none of these strains transferred trimethoprim resistance to E. coli K12. All the calves had received oral doses of 30 mg/kg day of trimethoprim-sulphadiazine for 5 consecutive days. From two subsequent batches of calves reared in the same shed, however, several isolations were made of E. coli with transmissible R factors determining trimethoprim and streptomycin resistance. Shortly before these strains were detected, isolations of E. coli with similar properties had been made from other calves, in a different shed, which had been fed much higher doses of trimethoprim-sulphadiazine. Serological evidence indicated that all the E. coli isolated carrying this R factor belonged to the same strain, which had apparently spread from the second shed to the first. No evidence of ‘in vivo’ transfer of the R factor to other enteric bacteria was obtained.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 4 , December 1973 , pp. 669 - 677
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Bushby, S. R. M. (1969). The combined antibacterial action in vitro of trimethoprim and sulphonamides. Postgraduate Medical Journal 45, Suppl. 10.Google ScholarPubMed
Datta, N. & Hedges, R. W. (1972). Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae. Journal of General Microbiology 72, 349.CrossRefGoogle Scholar
Fleming, M. P., Datta, N. & Grüneberg, R. N. (1972). Trimethoprim resistance determined by R factors. British Medical Journal 1, 726.CrossRefGoogle ScholarPubMed
Gillespie, W. A., Lee, P. A., Linton, K. B. & Rowland, A. J. (1971). Antibiotic resistance of coliform bacilli in urinary infections. Lancet ii, 675.CrossRefGoogle Scholar
Hedges, R. W. & Datta, N. (1973). Plasmids determining I pili constitute a compatibility complex. Journal of General Microbiology 77, 19.CrossRefGoogle ScholarPubMed
Hedges, R. W., Datta, N. & Fleming, M. P. (1972). R factors conferring resistance to trimethoprim but not sulphonamides. Journal of General Microbiology 73, 573.CrossRefGoogle Scholar
Hughes, D. T. C. (1969). Single-blind comparative trial of trimethoprim-sulphamethoxazole and ampicillin in the treatment of exacerbations of chronic bronchitis. British Medical Journal 4, 470.CrossRefGoogle ScholarPubMed
Kabbage, D., Ben, Brahim M. & Amine, M. (1971). Trial of trimethoprim-sulphamethoxazole in the pathology of infections and in particular in purulent meningitis, typhoid and paratyphoid. Journal Medical Marocain 7, 11.Google Scholar
Lacey, R. W., Gillespie, W. A., Bruten, D. M. & Lewis, E. L. (1972). Trimethoprim-resistant coliforms. Lancet i, 409.CrossRefGoogle Scholar
McAllister, T. A., Percival, A., Alexander, J. G., Boyce, J. M. H., Dulake, C. & Wormald, P. J. (1971). Multicentric study of sensitivities of urinary tract pathogens. Postgraduate Medical Journal 47, Suppl. 7.Google ScholarPubMed
Rail, W. & Kaller, H. (1971). A contribution from practice on the effectiveness of the trimethoprim/sulphonamide principle in Bavarian cattle holdings with bacterial problems in rearing. Praktische Tierarzt 52, 10Google Scholar
Rehm, W. F. & White, G. (1970). A field trial with trimethoprim and sulfadoxine in bacterial diseases of cattle and pigs. Veterinary Record 87, 39.CrossRefGoogle ScholarPubMed
Scholl, E., Bader, F., Campbell, D., Fluckiger, U., Gat, J., Glattli, H. R., Gonin, P., Hofer, A., Hilund-Carlsen, D., Meshorer, B. & Nesvadba, J. (1971). Veterinary trial of the chemotherapeutic preparation Borgal in infections of domestic animals in Switzerland, Denmark and Israel. Schweizer Archiv für Tierheilkunde 113, 387.Google Scholar
Seboulet, A. (1971). Gonococcal or non-gonococcal urogenital infections. Results of treatment with sulphamethoxazole-trimethoprim. Revue de Medecine 23, 1497.Google Scholar
Smith, H. W. & Halls, S. (1967). Observations by the ligated intestinal segment and oral inoculation methods on Escherichia coli infections in pigs, calves, lambs and rabbits. Journal of Pathology and Bacteriology 93, 499.CrossRefGoogle ScholarPubMed
Smith, H. W. & Linggood, M. A. (1972). Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. Journal of Medical Microbiology 5, 243.CrossRefGoogle Scholar
Sojka, W. J. (1965). E. coli in Domestic Animals and Poultry. Commonwealth Agricultural Bureaux.Google Scholar