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Toxin production and haemagglutination in strains of Escherichia coli from diarrhoea in Brescia, Italy

Published online by Cambridge University Press:  19 October 2009

R. Bisicchia ,
R. Ciammarughi ,
A. Caprioli ,
V. Falbo  and
F. M. Ruggeri
R. Bisicchia
Affiliation:
Laboratorio Analisi Chimico-cliniche e Microbiologia – Ospedale dei bambini ‘Umberto I’, Brescia
R. Ciammarughi
Affiliation:
Divisione Malatlie Infettive – Spedali Civili, Brescia
A. Caprioli
Affiliation:
Laboratorio di Ultrastrutture – Istituto Superiore di Sanità Viale Regina Elena, 299, 00161, Rome, Italy
V. Falbo
Affiliation:
Laboratorio di Ultrastrutture – Istituto Superiore di Sanità Viale Regina Elena, 299, 00161, Rome, Italy
F. M. Ruggeri
Affiliation:
Laboratorio di Ultrastrutture – Istituto Superiore di Sanità Viale Regina Elena, 299, 00161, Rome, Italy
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Two hundred and ninety-nine different strains of Escherichia coli, isolated from 172 patients with diarrhoea and 113 healthy subjects, were examined for enterotoxin, cytotoxin and haemolysin (Hly) production and for mannose-resistant haemagglutination (MRHA) and invasive properties. Three strains proved enterotoxigenic, none enteroinvasive; cytotoxin and Hly production was shown in 25 strains from patients and in 3 from controls. Ten strains produced the cytotoxic necrotizing factor (CNF), 6 released other factors which kill cell cultures. Hly production was shown in 21 strains, 9 of which were also positive for CNF. MRHA was detected in 26% of strains from diarrhoca compared with 14% of strains from healthy people. A strong association between toxin production and MRHA was demonstrated. Serotyping results showed that the strains exhibiting virulence traits mostly belonged to serogroups commonly involved in extra-intestinal infections. The possible role of strains of E. coli showing one or more virulence factors as opportunistic pathogens in diarrhoeal diseases is discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 2 , October 1985 , pp. 353 - 361
Copyright
Copyright © Cambridge University Press 1985

References

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