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Toxin production, adherence and protein expression by clinical Aeromonas spp. isolates in broth and human pooled ileostomy fluid

Published online by Cambridge University Press:  15 May 2009

M. H. Wilcox ,
A. Cook ,
I. Geary  and
A. Eley
M. H. Wilcox*
Affiliation:
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX
A. Cook
Affiliation:
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX
I. Geary
Affiliation:
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX
A. Eley
Affiliation:
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX
*
* Correspondence and current address: Dr M. H. Wilcox, Clinical Microbiology and Public Heath Laboratory, Level 6, Addenbrooks's Hospital, Hills Road, Cambridge CB2 2QW.
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The physiological behaviour of clinical Aeromonas spp. isolates was compared following culture in a conventional broth and human pooled ileostomy fluid (PIF). Protein expression was markedly affected by the growth medium, with an overall reduction in whole cell proteins in bacteria grown in ileostomy fluid. In addition, novel outer membrane proteins were produced in PIF but not in broth. The majority of A. hydrophila and A. sobria isolates produced toxin in both broth and PIF. whereas no cytotoxin positive A. caviae were found. Toxin titres were at least two doubling dilutions higher in 40% and 21% of A. hydrophila and A. sobria isolates, respectively, following culture in brain heart infusion broth compared with PIF. Bacterial adherence to Vero and A-549 cells was significantly more common in A. hydrophila (53%) and A. sobria (64%) than in A. caviae (15%) (P < 0·01). We observed increased adherence by 6 Aeromonas strains previously classified as adherence-positive, but not by 6 non-adherers, in PIF compared with brain heart infusion broth. The influence of growth medium on the expression of potential virulence determinants by Aeromonas spp. provides a rationale for the use of human ileostomy fluid in future in vitro studies, in order to simulate the nutrient conditions found in vivo.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 2 , October 1994 , pp. 235 - 245
Copyright
Copyright © Cambridge University Press 1994

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