Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-03T02:21:48.643Z Has data issue: false hasContentIssue false
  • English
  • Français

The titration of vaccinia virus on the chorio-allantoic membrane of the developing chick embryo

Published online by Cambridge University Press:  19 October 2009

J. C. N. Westwood ,
P. H. Phipps  and
E. A. Boulter
J. C. N. Westwood
Affiliation:
Microbiological Research Department, Porton, near Salisbury, Wilts
P. H. Phipps
Affiliation:
Microbiological Research Department, Porton, near Salisbury, Wilts
E. A. Boulter
Affiliation:
Microbiological Research Department, Porton, near Salisbury, Wilts
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A detailed investigation has been made of the pock-counting method for titrating vaccinia virus on embryonated eggs with a view to eliminating the wide scatter of results hitherto inseparable from the technique, and of assessing the precision of which it is capable. A detailed description is given of the technique evolved.

Reasons are given for supposing that the distribution of counts should follow the Poisson series, and considerable progress is claimed towards the achievement of such a distribution.

Type
Research Article
Information
Journal of Hygiene , Volume 55 , Issue 1 , March 1957 , pp. 123 - 139
Copyright
Copyright © Cambridge University Press 1957

References

Beveridge, W. I. B. & Burnet, F. M. (1946). The cultivation of viruses and rickettsiae in the chick embryo. Spec. Rep. Ser. med. Res. Coun.. Lond., no. 256.Google Scholar
Burnet, F. M. (1936 a). Immunologieal studies with the virus of infectious laryngotracheitis of fowls using the developing egg technique. J. exp. Med. 63, 685.CrossRefGoogle ScholarPubMed
Burnet, F. M. (1936 b). The use of the developing egg in virus research. Spec. Rep. Ser. med. Res. Coun., Lond., no. 220.Google Scholar
Burnet, G. M. & Faris, D. D. (1942). The techniques of quantitative chorioallantoic virus titration. J. Bact. 44, 241.CrossRefGoogle ScholarPubMed
Clarke, W. M. (1928). The Determination of Hydrogen Ions. 3rd ed. p. 214. London: Baillière, Tindall and Cox.Google Scholar
Dick, G. W. A. & Taylor, R. M. (1949). Bovine plasma albumin in buffered saline solution as a diluent for viruses. J. Immunol. 62, 311.CrossRefGoogle ScholarPubMed
Fenner, F. & McIntyre, G. A. (1956). Infectivity titrations of myxoma virus in the rabbit and the developing chick embryo. J. Hyg. Camb., 54, 246.CrossRefGoogle ScholarPubMed
Keogh, E. V. (1936). Titration of vaccinia virus on the chorioallantoic membrane of the chick embryo and its application to immunological studies of neurovaccinia. J. Path. Bact. 43. 441.CrossRefGoogle Scholar
Nadeje, T., Tamm, I. & Overman, J. R. (1955). A new technique for dropping the chorio-allantoic membrane in embryonated chicken eggs. J. Lab. clin. Med. 46, 648.Google Scholar
Overman, J. R. & Tamm, I. (1956). Quantitative titration of vaccinia virus on the chorio-allantoic membrane. J. Immunol. 76, 228.CrossRefGoogle Scholar
Pereira, H. G. (1954). The use of suspensions of isolated cells for the study of the factors affecting the multiplication of fowl-plague virus. J. gen. Microbiol. 10, 500.CrossRefGoogle Scholar
Reid, D. B. W., Crawley, J. F. & Rhodes, A. J. (1949). A study of fowl pox virus titration on the chorioallantois by the pock counting technique. J. Immunol. 63, 165.CrossRefGoogle Scholar
Skinner, H. H. & Bradish, C. J. (1954). Exposure to light as a source of error in the estimation of the infectivity of virus suspensions. J. gen. Microbiol. 10, 377.CrossRefGoogle ScholarPubMed
Woodruff, A. M. & Goodpasture, E. W. (1931). The susceptibility of the chorioallantoic membrane of chick embryos to infection with the fowl-pox virus. Amer. J. Path. 7, 209.Google ScholarPubMed