Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-25T18:41:42.184Z Has data issue: false hasContentIssue false
  • English
  • Français

Titration of the virus of foot-and-mouth disease in culture

Published online by Cambridge University Press:  15 May 2009

J. B. Brooksby  and
Ella Wardle
J. B. Brooksby
Affiliation:
Research Institute (Animal Virus Diseases), Pirbright, Surrey
Ella Wardle
Affiliation:
Research Institute (Animal Virus Diseases), Pirbright, Surrey
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A technique is presented for the titration of the virus of foot-and-mouth disease in culture in surviving epithelial tissue from the tongues of cattle. The cultures are incubated in cups on Perspex plates, and the detection of virus multiplication is by a complement-fixation test made on the culture in each cup.

On the basis of comparative titrations in culture and in cattle, the method has been found to be as sensitive for the detection of virus as the titration by intradermal inoculation of the tongue of cattle. The method can also be applied in the detection of antibody in neutralization tests.

We wish to record our thanks to Messrs E. Scoates and P. Mitchell for their technical assistance, and to Messrs H. M. Smith, R. H. Compton and R. L. Jackson for their part in the design and fabrication of various bottle rotators, plate shakers, Perspex lids and the inoculating box.

Type
Research Article
Information
Journal of Hygiene , Volume 52 , Issue 1 , March 1954 , pp. 87 - 99
Copyright
Copyright © Cambridge University Press 1954

References

REFERENCES

Brooksby, J. B. (1952). The technique of complement-fixation in foot-and-mouth disease A.R.C. Rep. Ser. no. 12. London: H.M. Stationery Office.Google Scholar
Fisher, R. A. & Yates, F. (1948). Statistical Tables, 3rd ed. Edinburgh: Oliver and Boyd.Google Scholar
Frenkel, H. S. (1947). La culture du Virus de la Fièvre Aphteuse sur l'Epithelium de la Langue des Bovidés. Bull. Off. int. Epiz. 28, 155.Google Scholar
Frenkel, H. S. & Dunne, H. W. (1952). Research on foot-and-mouth disease. Cultivation of the foot-and-mouth disease virus in explanted epithelium of the bovine tongue IV. Tissue and medium variation. Amer. J. vet. Res. 13, 21.Google Scholar
Fulton, F. & Armitage, P. (1951). Surviving tissue suspensions for influenza virus titration. J. Hyg., Camb., 49, 247.Google ScholarPubMed
Henderson, W. M. (1949). The quantitative study of foot-and-mouth disease virus. A.R.C. Rep. Ser. no. 8. London: H.M. Stationery Office.Google Scholar
Henderson, W. M. (1953). Nature of virus multiplication. 2nd Symp. Soc. gen. Microbiology, Discussion, p. 317. Cambridge University Press.Google Scholar
Huang, C. H. (1943). Further studies on the titration and neutralization of the western strain of equine encephalomyelitis virus in tissue culture. J. exp. Med. 78, 111.CrossRefGoogle ScholarPubMed
Ledinko, N., Riordan, J. T. & Melnick, J. L. (1951). Differences in cellular pathogenicity of two immunologically related poliomyelitis viruses as revealed in tissue culture. Proc. Soc. exp. Biol., N.Y., 78, 83.CrossRefGoogle ScholarPubMed
Parker, R. C. (1938). Methods of Tissue Culture. New York: Hoeber.Google Scholar
Reed, L. J. & Muench, H. (1938). A simple method of estimating fifty per cent end-points. Amer. J. Hyg. 27, 493.Google Scholar