Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-22T06:48:25.590Z Has data issue: false hasContentIssue false
  • English
  • Français

Susceptibility of various animals to the vesiculoviruses Isfahan and Chandipura

Published online by Cambridge University Press:  19 October 2009

C. R. Wilks  and
J. A. House
C. R. Wilks
Affiliation:
Plum Island Animal Disease Center (PIADC), United States Department of Agriculture, ARS, S & E, PO Box 848, Greenport, New York, 11944
J. A. House
Affiliation:
Plum Island Animal Disease Center (PIADC), United States Department of Agriculture, ARS, S & E, PO Box 848, Greenport, New York, 11944
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

To determine the pathogenic potential of the vesiculoviruses Isfahan Chandipura for domestic animals, two ponies, two steers, three and three pigs were inoculated with each virus intradermally in the tongue or, in the case of the pigs, in the snout, heel and coronary band The Ponies were also inoculated intradcrmally in the right commissure o the mouth Animals inoculated with each virus were housed in one room and allowed to mingle freely with an equal number of uninoculated contact animals of each species.

Clinical signs of infection, consisting of ulcers at the inoculation sites, were observed in the Chandipura study in two inoculated ponies, one inoculated steer and one inoculated goat. No elevated temperature was observed. Virus was isolated from the ulcerated tongue tissue but not from serial blood samples, oesophagcal-pharyngeal mucus samples, or from the tissues which were collected at necropsy. Precipitating antibody was not detected by the immunoelectro osmophoresis(IEOP) test in any of the pre- or post-serum samples except from two inoculated sheep at 29 days post-inoculation (D.P.I.). Low levels of neutralizing activity were dtedted in pre-inoculation serum from all steers, pigs, contact sheep, and one contact goa. By 15 D.P.I. all inoculated animals and contact ponies and steers exhibited increased neutralizing antibody titres.

In studies with the Isfahan virus, lesions developed only at the inoculation sites in the two ponies, and the virus was isolated. No virus was isolated from any blood, oesophageal-pharyngeal mucus samples or tisues collected at necropsy. All pre-inoculation sera were negative for neutralizing and precipitating antibodies. By 14 D.P.I. all inoculated animals exhibited neutralizing antibody, while all the contacts remained negative. The IEOP test remained negative for all animals throughout the experiment. A subpassage of a suspension of Isfahan-infected tongue tissue injected in to ponies and steers also yielded only firm swellings of lesser extent than the original reaction at the inoculation sites.

With both viruses, lethal infections were produced by intraacranial or intraperitoneal inoculation of day-old mice and hamsters, and by allantoic inoculation of embryonating chicken eggs. Adult mice, hamsters, guinea-pigs and rabbits produced serum antibodies but lacked clinical signs.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 2 , October 1986 , pp. 359 - 368
Copyright
Copyright © Cambridge University Press 1986

References

Anon. (1967). Catalogue of Arthropod-borne Viruses of the World, pp. 208–209. Washington, D.C.: Public Health Publication no. 1760.Google Scholar
Berge, T. O. (1975). International Catalogue of Arboviruses Including Certain other Viruses of Vertebrates, pp. 208–209, Bethesda, Maryland: U.S. Department of Health, Education and Welfare Publication no. 75–8301.Google Scholar
Bhatt, P. D. & Rodrigues, F. M. (1967). Chandipura: a new arbovirus isolated in India from patients with febrile illness. Indian Journal of Medical Research 55, 1295–1305.Google ScholarPubMed
Brown, R., Bishop, D. H. L., Crick, J., Francki, R. I. B., Holland, J. J., Hull, R., Johnson, K., Martelli, G., Obijeski, J. F., Peters, D., Prinole, C. R., Reichmann, M. E., Schneider, L. G., Shope, R. E., Simpson, D. I. H., Summers, E. F. & Wagner, R.R. (1979).Rhabdoviridae. Intervirology 12, 1–7.CrossRefGoogle ScholarPubMed
Federer, K. E., Burrows, R. & Brooksby, J. B. (1967). Vesicular stomatitis virus – the relationship between some strains of the Indiana serotype. Research in Veterinary Science 8, 103–117.CrossRefGoogle ScholarPubMed
Finney, D. J. (1978). Statistical Method in Biological Assay, 3rd edn, p. 220. New York: MacMillan.Google Scholar
Hanson, R. P. (1981). Diseases of Swine (ed. Leman, A. D., Glock, R. D., Mengeling, W. L., Penny, R. H. C., Scholl, E. and Straw, B.), pp. 294–301. Ames: Iowa State University Press.Google Scholar
House, J. A. & Wilks, C. R. (1983). Studies on exotic vesiculoviruses. Proceedings of the 87th Annual Meeting of the United States Animal Health Association, pp. 276–288.Google Scholar
Mills, B. J., Beebe, D. P. & Cooper, N. R. (1979). Antibody independent neutralization of vesicular stomatitis virus by human complement. II. Formation of VSV-liprotein complexes in human serum and complement-dependent virus lysis. Journal of Immunology 123, 2518–2524.CrossRefGoogle Scholar
Rao, T. R., Singh, K. R. P., Dhanda, V. & Bhatt, P. N. (1967). Experimental transmission of Chandipura virus by mosquitoes. Indian Journal of Medical Research 55, 1306–1310.Google ScholarPubMed
Tesh, R., Saidi, S., Javadian, E., Loh, P. & Nadim, A. (1977). Isfahan virus, a new vesiculovirus infecting humans, gerbils, and sandfiies in Iran. American Journal of Tropical Medicine and Hygiene 26, 299–306.CrossRefGoogle Scholar
Tesh, R. B., Travassos da Rosa, A. P. A. & Travassos da Rosa, J. S. (1983). Antigenic relationship among rhabdoviruses infecting terrestrial vertebrates. Journal of General Virology 64, 169–176.CrossRefGoogle ScholarPubMed
Wilks, C. R. & House, J. A. (1984). Susceptibility of various animals to the vesiculovirus Piry. Journal of Hygiene 93, 147–155.CrossRefGoogle Scholar
Wilks, C. R. & House, J. A. (1985). The glycoproteins of seven vesiculoviruses are antigenically distinct. Archives of Virology 86, 335–340.CrossRefGoogle ScholarPubMed
Wilks, C. R., Jenny, E. W. & House, J. A. (1984).Development of an immunoelectroosmophoresis test for the detection and typing of antibodies to vesicular stomatitis viruses. Canadian Journal of Comparative Medicine 48, 179–183.Google ScholarPubMed