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A survey on the occurrence of Vibrio parahaemolyticus on fish and shellfish, marketed in The Netherlands

Published online by Cambridge University Press:  15 May 2009

E. H. Kampelmacher ,
D. A. A. Mossel ,
L. M. van Noorle Jansen  and
H. Vincentie
E. H. Kampelmacher
Affiliation:
National Institute of Public Health, Utrecht, The Netherlands
D. A. A. Mossel
Affiliation:
National Institute of Public Health, Utrecht, The Netherlands
L. M. van Noorle Jansen
Affiliation:
Central Institute for Nutrition & Food Research T.N.O., Zeist, The Netherlands
H. Vincentie
Affiliation:
Central Institute for Nutrition & Food Research T.N.O., Zeist, The Netherlands
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A survey was carried out on the occurrence of Vibrio parahaemolyticus on fish and shellfish, as sold in The Netherlands.

The optimal mode of detection of this bacterium appeared to be: (i) enrichment of swabs taken from the surface and the gills in freshly prepared meat broth with 5% NaCl; (ii) streaking onto Teepol bromothymol blue agar (BTB) and taurocholate bromothymol blue sucrose agar (TCBS); (iii) confirmation of suspect colonies by testing for mode of growth in butts/slants of a Kligler type glucose sucrose iron thiosulphate agar, formation of indole in 2% NaCl 2% trypticase water, anaerobic utilization of starch in the presence of 5% NaCl and oxidase reaction according to Kovacs (1956).

A total of 407 samples, stemming from 17 types of fish and shellfish, taken at three fish shops, was examined by this technique. Only one specimen, i.e. a haddock, was found to contain V. parahaemolyticus. This contamination rate of approximately 0·3% correlates well with data found earlier for fish landed in Northern Germany.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 2 , June 1970 , pp. 189 - 196
Copyright
Copyright © Cambridge University Press 1970

References

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