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Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction

Published online by Cambridge University Press:  15 May 2009

H. Wu ,
K. Taniguchi ,
F. Wakasugi ,
S. Ukae ,
S. Chiba ,
M. Ohseto ,
A. Hasegawa ,
Tomoko Urasawa  and
Shozo Urasawa
H. Wu
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
K. Taniguchi
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
F. Wakasugi
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
S. Ukae
Affiliation:
Department of Pediatrics, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
S. Chiba
Affiliation:
Department of Pediatrics, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
M. Ohseto
Affiliation:
Ehime Institute of Public Health, Matsuyama, Japan
A. Hasegawa
Affiliation:
Department of Epidemiology, National Institute of Health, Tokyo, Japan
Tomoko Urasawa
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
Shozo Urasawa
Affiliation:
Department of Hygiene, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
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Summary

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The presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the four representative gene 4 alleles. Using the PCR. we surveyed the gene 4 alleles of 199 human rotaviruses in stools collected in Japan and Thailand. Strains with the gene 4 allele, corresponding to P1A serotype. were shown to be the most prevalent, but two strains with P2 gene 4 allele and one strain with P3 gene 4 allele were detected in Thailand and in Japan, respectively.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 3 , June 1994 , pp. 615 - 622
Copyright
Copyright © Cambridge University Press 1994

References

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