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A surface antigen influenza vaccine. 1. Purification of haemagglutinin and neuraminidase proteins

Published online by Cambridge University Press:  15 May 2009

Margaret I. Brady  and
I. G. S. Furminger
Margaret I. Brady
Affiliation:
Evans Biologicals Limited, Speke, Liverpool, L24 9JD
I. G. S. Furminger
Affiliation:
Evans Biologicals Limited, Speke, Liverpool, L24 9JD
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Influenza virus was centrifuged in a KII rotor through a sucrose gradient containing Triton N101, a non-ionic surfactant. The micelles of surfactant formed a band in the gradient. As virus particles passed through the surfactant, the haemagglutinin and neuraminidase proteins were stripped from the surface and remained near the surfactant micelles. The residual virus particles sedimented into a denser region of the gradient and were thus separated from the haemagglutinin and neuraminidase antigens. Fractions containing the surface antigens were pooled and Triton was removed by phase-separation at the cloud point.

Type
Research Article
Information
Journal of Hygiene , Volume 77 , Issue 2 , October 1976 , pp. 161 - 172
Copyright
Copyright © Cambridge University Press 1976

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