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A study of the surface and somatic antigens of Bacteroides fragilis

Published online by Cambridge University Press:  15 May 2009

K. M. Elhag  and
Soad Tabaqchali
K. M. Elhag
Affiliation:
Department of Medical Microbiology, St Bartholomew's Hospital, London EC1A 7BE
Soad Tabaqchali
Affiliation:
Department of Medical Microbiology, St Bartholomew's Hospital, London EC1A 7BE
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Summary

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Antisera against live cultures of 20 strains of Bacteroides fragilis produced in rabbits agglutinated homologous whole-cell as well as O-antigen suspensions with several cross-reactions. Pure specific antisera were produced by absorbing cross-reacting antigens. O-antisera reacted against O-antigens but failed to do so against most whole-cell suspensions, suggesting the presence of surface structures which might have blocked the reactions. The titres of the whole-cell antisera were not significantly higher than those of O-antisera, but the reactions were more definite and easier to read.

This system may prove useful in serological studies of B. fragilis.

Type
Research Article
Information
Journal of Hygiene , Volume 80 , Issue 3 , June 1978 , pp. 439 - 449
Copyright
Copyright © Cambridge University Press 1978

References

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