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A study of a hospital cluster of systemic candidosis using DNA typing methods

Published online by Cambridge University Press:  15 May 2009

F. Romano
Affiliation:
Institute of Hygiene, Medical School, University of Chieti ‘G. D'Annunzio’, Via dei Vestini, 66013 Chieti Scalo, Italy
G. Ribera
Affiliation:
Institute of Hygiene and Preventive Medicine, Medical School, University of Naples‘Federico II’, Italy
M. Giuliano
Affiliation:
Institute of Hygiene and Preventive Medicine, Medical School, University of Naples‘Federico II’, Italy
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Summary

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A cluster of disseminated Candida albicans infections, which occurred at the Intensive Care Unit of the Department of Heart Surgery, was investigated. Ten patients became infected and seven died. A wide microbiological surveillance was carried out. A total of 14 isolates of Candida albicans, four environmental and ten human, were examined using the Restriction Endonuclease Analysis (REA) of DNA. The isolates were classified into five different main groups. Five of the clinical isolates had the predominant pattern Ab and two more clinical strains were very closely related. Two more isolates from the emergency kit desk and the hands of a nurse gave the same REA profile. Such a relationship proved the epidemic nature of the cluster, with most of the patients cross-infected, and strongly suggested transmission on the hands of the staff as a determinant of the epidemic. Thus, REA has the potential to address many important questions in the study of nosocomial epidemiology of Candida albicans.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

Burnie, JP, Odds, FC, Lee, W, Webster, C, Williams, JD. Outbreak of systemic Candida albicans in intensive care unit caused by cross–infection. BMJ 1985: 290: 746–8.CrossRefGoogle ScholarPubMed
Burnie, JP, Matthews, R, Lee, W et al. Four outbreaks of nosocomial systemic candidiasis. Epidemiol Infect 1987; 99: 201–11CrossRefGoogle ScholarPubMed
Lee, W, Burnie, JP, Matthews, R, Oppenheim, BO, Damani, N. Hospital outbreaks with yeasts. J Hosp Infect 1991; 18: 237–49.CrossRefGoogle ScholarPubMed
Fox, BC, Mobley, HLT, Wade, JC. The use of a DNA probe for epidemiological studies of candidiasis in immunocompromised hosts. Infect Dis 1989; 159: 488–94.CrossRefGoogle ScholarPubMed
Scherer, S, Stevens, DA. Application of Dna typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol 1987; 25: 675–9.CrossRefGoogle ScholarPubMed
Merz, WG. Candida albicans strain delineation. Microbiol Rev 1990: 54: 321–34.CrossRefGoogle Scholar
Jordens, JZ. Restriction enzyme analysis of chromosomal DNA its application in epidemiologicalstudies. J Hosp Infect 1991; 18 Suppl. A: 432–7.CrossRefGoogle Scholar
Warnock, DW. Typing ofCandida albicans. J Hosp Infect 1984: 5: 244–52.CrossRefGoogle Scholar
Stevens, DA, Odds, FC, Scherer, S. Application of Dna typing methods to Candida albicans epidemiology and correlations with phenotype. Rev Infect Dis 1990; 12: 258–66.CrossRefGoogle ScholarPubMed
Matthews, RC, Burnie, JP. Assessment of Dna fingerprinting for rapid identification of outbreaks of systemic candidiasis. BMJ 1989; 298: 354–7.CrossRefGoogle ScholarPubMed
Scherer, S, Magee, PT. Genetics of Candida albicans. Microbiol Rev 1990; 54: 226–41.CrossRefGoogle ScholarPubMed
Pfaller, MA. The use of molecular techniques for epidemiologic typing ofCandida species. Curr Top Med Mycol 1992; 4: 4363.CrossRefGoogle Scholar
Magee, BB, D'Souza, TM, Magee, PT. Strain andspecies identification by restriction fragment length polymorphisms in the ribosomal Dna repeat of Candida species. J Bacteriol 1987; 169: 1639–43.CrossRefGoogle Scholar
Carruba, G, PontieriE, De E, DeBernardis, F, Martino, P.Cassone, A. Dna fingerprinting electrophoretic karyotype of environmental and clinical isolates ofCandida parapsilosis. J Clin Microbiol 1991; 29: 916–22.CrossRefGoogle Scholar
Oren, I, Manavathu, EK, Lerner, SA. Isolation and characterization of a species–specific DNA probe forCandida albicans. Nucleic Acids Res 1991; 19: 7113–6.CrossRefGoogle ScholarPubMed
Wilkinson, BM, Morris, L, Adams, DJ, Evans, EG, Lacey, CJ.Walmsey, RM. A new sensitive polynucleotide probe for distinguishing Candida albicans strains and its use with a computer assisted archiving and pattern com paris on system. J Med Vet Mycol 1992: 30: 123–31.CrossRefGoogle Scholar