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Studies with Marked Antisera 2. Variation in the Specific Adsorption by Red Cells from Different Rabbits of One 131I-marked rabbit ISO-Antiserum

Published online by Cambridge University Press:  15 May 2009

J. C. Boursnell ,
D. H. Heard  and
Vivien Rizk
J. C. Boursnell
Affiliation:
Departments of Biochemistry and Pathology, University of Cambridge
D. H. Heard
Affiliation:
Departments of Biochemistry and Pathology, University of Cambridge
Vivien Rizk
Affiliation:
Departments of Biochemistry and Pathology, University of Cambridge
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1. An apparently pure rabbit iso-antibody marked with 131I has been shown to adsorb specifically to a greater extent on some rabbit cells than on others. This accords with previous evidence that the same iso-antibody exhibits a higher titre with the cells which are shown here to absorb more marked antibody than with those which adsorb less.

2. Saturation of two of these red cell types by repeated application of fresh quantities of the 131I iso-antibody until no more is specifically adsorbed, suggests that the number of functional antigenic sites is six or seven times greater on the one than on the other cell.

3. There is no apparent difference in the amount of 131I iso-antibody adsorbed by any one type of rabbit cell in comparative titrations using 3·3 % MgSO4.7H2O medium instead of normal saline.

Type
Research Article
Information
Journal of Hygiene , Volume 53 , Issue 4 , December 1955 , pp. 420 - 425
Copyright
Copyright © Cambridge University Press 1955

References

Boursnell, J. C., Coombs, R. R. A. & Rizk, V. (1953). Studies with marked antisera. Quantitative studies with antisera marked with iodine 131isotope and their corresponding red cell antigens. Biochem. J. 55, 745.Google Scholar
Heard, D. H. (1955 a). The recognition of four red cell antigen-antibody systems in the rabbit. J. Hyg., Camb., 53, 398.Google Scholar
Heard, D. H. (1955 b). Factors influencing the agglutinability of red cells. Variation of the red cells of the rabbit in susceptibility to agglutination by homologous iso-antisera. J. Hyg., Camb., 53, 408.Google Scholar
Heard, D. H., Hinde, I. T. & Mynors, L. S. (1949). An experimental study of haemotitic disease of the newborn due to iso-immunization of pregnancy. I. An attempt to produce the disease in the rabbit. J. Hyg., Camb., 47, 119.Google Scholar