Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2025-01-05T11:39:38.117Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions

Published online by Cambridge University Press:  15 May 2009

Janet M. Bradbury  and
F. T. W. Jordan
Janet M. Bradbury
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station, ‘Leahurst’, Neston, Wirral, Cheshire
F. T. W. Jordan
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station, ‘Leahurst’, Neston, Wirral, Cheshire
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Serum proteins from Mycoplasma gallisepticum culture medium could be detected on the organisms as a result of incubation at low pH. Only certain of the serum proteins, including IgG and IgM, were found, and the adsorption appears to depress the haemagglutinating activity of the organisms. There was no obvious effect on slide agglutination (SA) sensitivity but an incidental finding was that brief acid treatment enhanced the SA sensitivity of an antigen prepared from a young culture.

Type
Research Article
Information
Journal of Hygiene , Volume 70 , Issue 2 , June 1972 , pp. 267 - 278
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Asmar, J. A. (1965). Chromatographic and immunoelectrophoretic characterization of chicken and turkey agglutinating antibodies to Mycoplasma gallisepticum. Ph.D. Thesis, University of California.Google Scholar
Beernink, K. D. & Steward, J. P. (1967). Proteins adhering to Escherichia coli after exposure to guinea-pig serum. Immunology 12, 207.Google ScholarPubMed
Bradbury, J. M. & Jordan, F. T. W. (1971 a). Investigation into rabbit infusion media for the growth of Mycoplasma gallisepticum antigens for inoculation into rabbits. Journal of Hygiene 69, 73.CrossRefGoogle ScholarPubMed
Bradbury, J. M. & Jordan, F. T. W. (1971 b). The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests. Journal of Hygiene 69, 593.Google ScholarPubMed
Bradbury, J. M. & Jordan, F. T. W. (1971 c). The adsorption of gamma globulins to Mycoplasma gallisepticum and the possible role in non-specific serological reactions. Veterinary Record 89, 318.CrossRefGoogle Scholar
Crowle, A. J. (1961). Immunodiffusion, p. 306. New York: Academic Press.Google Scholar
Hamburger, R. N., Pious, D. A. & Mills, S. E. (1963). Antigenic specificities acquired from the growth medium by cells in tissue culture. Immunology 6, 439.Google ScholarPubMed
Herbert, W. J. (1967). Handbook of Experimental Immunology (ed. Weir, D. M.), p. 1211. Oxford: Blackwell Scientific Publications.Google Scholar
Hirata, A. A. & Stashak, P. W. (1965). Simplified procedure for passive haemagglutination tests with protein antigens. Nature, London 205, 1233.CrossRefGoogle Scholar
Jordan, F. T. W. & Kulasegaram, P. (1968). Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum. Journal of Comparative Pathology 78, 407.Google Scholar
King, C. A. (1968). A general study on the adsorption of protein by tissue. Biochimica et Biophysica Acta 154, 269.Google Scholar
Neimark, H. C. & Pickett, M. J. (1960). Products of glucose metabolism by pleuropneumonia-like organisms. Annals of the New York Academy of Sciences 79, 531.CrossRefGoogle Scholar
Nutor, B. L. (1969). The isolation of avian mycoplasma from field material and the serological response of turkeys to M. gallisepticum. M.V.Sc. Thesis, University of Liverpool.Google Scholar
Scheidegger, J. J. (1955). Une microméthode de l'immunoelectrophorèse. International Archives of Allergy and Applied Immunology 7, 103.CrossRefGoogle Scholar
Smith, S. C., Dunlop, W. R. & Strout, R. G. (1966). Effect of culture medium on antigenic structure of mycoplasma. Avian Diseases 10, 173.CrossRefGoogle ScholarPubMed
Tormo, J., Chordi, A., Rodriguez-Burgos, A. & Diaz, R. (1967). Immunoelectrophoresis of normal pig serum. Veterinary Record 81, 392.Google Scholar
Tourtellotte, M. E. & Jacobs, R. E. (1960). Physiological and serological comparisons of PPLO from various sources. Annals of the New York Academy of Sciences, 79, 521.Google Scholar