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A standardized monitor for the control of ethylene oxide sterilization cycles

Published online by Cambridge University Press:  19 October 2009

A. H. Dadd ,
Celia M. Stewart  and
Margaret M. Town
A. H. Dadd
Affiliation:
Bacteriological Laboratories, Imperial College of Science and Technology, London, SW7 2BB, U.K.
Celia M. Stewart
Affiliation:
Bacteriological Laboratories, Imperial College of Science and Technology, London, SW7 2BB, U.K.
Margaret M. Town
Affiliation:
Bacteriological Laboratories, Imperial College of Science and Technology, London, SW7 2BB, U.K.
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Summary

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The resistance of spores of B. subtilis var. niger produced in liquid synthetic medium and exposed to ethylene oxide on a nylon surface, has been shown to be almost identical to that for spores produced on a traditional solidified complex medium with exposure to the sterilant on aluminium foil. The use of short lengths of nylon tube as carriers allowed easy production and handling, with self-protection of the spore-bearing surface. Addition of a dye provided visual evidence of inoculation without affecting resistance to ethylene oxide. Such a monitor is suitable for use as a standardized biological challenge in routine ethylene oxide sterilization cycles.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 1 , August 1983 , pp. 93 - 100
Copyright
Copyright © Cambridge University Press 1983

References

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