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The Standardization of killed Agglutinable Cultures of B. dysenteriae

Published online by Cambridge University Press:  15 May 2009

A. D. Gardner
A. D. Gardner
Affiliation:
(From the Standards Laboratory, Department of Pathology, University of Oxford.)
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(1) Killed agglutinable broth cultures of the various dysentery bacilli may be prepared by the formalin and cold storage process and may be standardized by the method elaborated by Prof. Dreyer for the typhoid and paratyphoid bacilli.

(2) Since formalin (0·1 %) usually causes a gradual loss of sensitiveness to agglutination during the early life of the emulsion, a certain period (two months) must be allowed to elapse before the emulsion can be used as standard.

(3) After such a period the sensitiveness of the emulsions remains unaltered for at least ten months, and probably much longer.

(4) By none of the other methods of preparation investigated could satisfactory standard agglutinable cultures be made.

Type
Research Article
Information
Journal of Hygiene , Volume 17 , Issue 4 , October 1918 , pp. 471 - 502
Copyright
Copyright © Cambridge University Press 1918

References

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