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Stability of aminoglycoside resistance in vitro in gentamicin-resistant Staphylococcus aureus

Published online by Cambridge University Press:  19 October 2009

Shirley Dixson ,
W. Brumfitt  and
J. M. T. Hamilton-Miller
Shirley Dixson
Affiliation:
Department of Medical Microbiology, Royal Free Hospital and School of Medicine, Pond Street, Hampstead, London NW3
W. Brumfitt
Affiliation:
Department of Medical Microbiology, Royal Free Hospital and School of Medicine, Pond Street, Hampstead, London NW3
J. M. T. Hamilton-Miller
Affiliation:
Department of Medical Microbiology, Royal Free Hospital and School of Medicine, Pond Street, Hampstead, London NW3
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Stability of aminoglycoside resistance has been investigated in 20 strains of Staphylococcus aureus resistant to gentamicin (16 strains were also resistant to methicillin). In view of previous reports that incubation at elevated temperatures can hasten the loss of unstable antibiotic resistance, we passaged strains daily in a liquid medium for 24 days at 43 °C. The nine strains which were resistant to neomycin kept their aminoglycoside resistance virtually intact, whereas most of the other 11 strains (sensitive to neomycin) lost almost all their resistance to gentamicin and kanamycin after 5 days. It thus appears that the stability of aminoglycoside resistances in Staph. aureus is closely linked to the resistance of the strains to neomycin. This finding has important possible consequences in terms of the advisability of the clinical usage of preparations containing neomycin or framycetin for topical application and bowel sterilization.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 1 , August 1984 , pp. 43 - 49
Copyright
Copyright © Cambridge University Press 1984

References

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