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Specific IgE response in patients with brucellosis

Published online by Cambridge University Press:  15 May 2009

G. F. Araj ,
A. R. Lulu ,
M. I. Khateeb  and
M. Haj
G. F. Araj
Affiliation:
Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923Kuwait13110
A. R. Lulu
Affiliation:
Department of Medicine, Jahra Hospital, MPH, Jahra, Kuwait
M. I. Khateeb
Affiliation:
Department of Medicine, Jahra Hospital, MPH, Jahra, Kuwait
M. Haj
Affiliation:
Department of Microbiology, Faculty of Medicine, Kuwait University, P.O. Box 24923Kuwait13110
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Summary

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In the search to find discriminative serological markers to differentiate between patients with acute brucellosis and those with chronic brucellosis, an enzyme-linked immunosorbent assay (ELISA) was used to determine and compare the brucella-specific IgE response in 80 sera from patients with acute brucellosis, 37 sera from patients with chronic brucellosis, 26 sera from patients with positive blood cultures for bacteria other than brucella and 51 sera from healthy controls. The IgE findings were compared to brucella-specific IgG, IgM, IgA and IgG1–4 demonstrated by ELISA, and to microagglutination test (MAT) results. Elevated (positive) antibrucella IgE titres were detected in 89 and 81 % of sera from patients with acute and chronic brucellosis respectively. The predominant antibodies found in patients with acute brucellosis were of the IgG, IgM, IgA, IgE, IgG1 and IgG3 types while in chronic brucellosis IgG, IgA, IgE and IgG4 were found. Although IgE can be detected in patients with brucellosis, it does not discriminate between the acute and chronic stages of the disease.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 3 , December 1990 , pp. 571 - 577
Copyright
Copyright © Cambridge University Press 1990

References

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