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Species identification, antibiotic sensitivity and slime production of coagulase-negative staphylococci isolated from clinical specimens

Published online by Cambridge University Press:  15 May 2009

M. A. Deighton ,
J. C. Franklin ,
W. J. Spicer  and
B. Balkau
M. A. Deighton
Affiliation:
Department of Applied Biology, Royal Melbourne Institute of Technology, Australia
J. C. Franklin
Affiliation:
Department of Bacteriology, Alfred Hospital, Melbourne, Australia
W. J. Spicer
Affiliation:
Department of Bacteriology, Alfred Hospital, Melbourne, Australia
B. Balkau
Affiliation:
Department of Mathematics, Royal Melbourne Institute of Technology, Australia
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Two hundred and seventy-five consecutive clinical isolates of coagulase-negative staphylococci, including strains associated with disease, contaminants and skin colonizers, were speciated, tested for slime production and for their sensitivity to a range of antibiotics. Staphylococcus epidermidis was the most commonly identified species, comprising 63% of all isolates. Slime production was detected in half the strains of Staph. epidermidis, Staph. haemolyticus and Staph. Saprophyticus but was rare in other species. Most Staph. haemolyticus strains and approximately half of the Staph. epidermidis strains were resistant to five or more antibiotics. A significant association was found between slime production and multiple antibiotic resistance. For catheter-associated strains, clinical relevance was predictable by species i.e. Staph. epidermidis. Multi-resistant slime-positive Staph. haemolyticus strains, although infrequently associated with disease, were common skin colonizers, presumably acquired from the hospital environment. We describe a practical and inexpensive scheme for thespeciation of human coagulase-negative staphylococcal isolates.

Type
Special Article
Information
Epidemiology & Infection , Volume 101 , Issue 1 , August 1988 , pp. 99 - 113
Copyright
Copyright © Cambridge University Press 1988

References

REFERENCES

Annear, D. I. (1968). The effect of temperature on resistance of Staphylococcus aureus to methicillin and some other antibiotics. Medical Journal of Australia 1, 444–446.CrossRefGoogle Scholar
Archer, G. L. & Johnston, J. L. (1983). Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides. Antimicrobial agents and Chemotherapy 24, 70–77.CrossRefGoogle ScholarPubMed
Baddour, L. M., Christensen, G. D., Hester, M. G. & Bisno, A. L. (1984). Production of experimental endocarditis by coagulase-negative staphylococci: variability in species virulence. The Journal of Infectious Diseases 150, 721–727.CrossRefGoogle ScholarPubMed
Barber, M. & Kuper, S. W. A. (1951). Identification of Staphylococcus pyogenes by the phosphatase reaction. Journal of Pathology and Bacteriology 63, 65–68.CrossRefGoogle ScholarPubMed
Bayston, R. & Penny, S. R. (1972). Excessive production of mucoid substance in Staphylococcus S11A: a possible factor in colonisation of Holter shunts. Developmental Medicine and Child Neurology 14, (Suppl. 27), 25–28.Google Scholar
Butcher, R., Franklin, J. C., Gilbert, G., Olden, D., Stockman, K. & Ward, P. (1986). Agar dilution susceptibility testing for bacteria that grow in air. Clinical Microbiology Update Programme (ed. Beaton, C.), no. 27. Australian Society for Microbiology.Google Scholar
Christensen, G. D., Bisno, A. L., Parisi, J. T., McLaughlin, B., Hester, M. G. & Luther, R. W. (1982 a). Nosocomial septicemia due to multiply antibiotic resistant Staphylococcus epidermidis. Annals of Internal Medicine 96, 1–10.CrossRefGoogle ScholarPubMed
Christensen, G. D., Simpson, W. A., Bisno, A. L. & Beachey, E. H. (1982 b). Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infection and Immunity 37, 318–326.CrossRefGoogle ScholarPubMed
Christensen, G. D., Parisi, J. T., Bisno, A. L., Simpson, W. A. & Beachey, E. H. (1983). Characterization of clinically significant strains of coagulase-negative staphylococci. Journal of Clinical Microbiology 18, 258–269.CrossRefGoogle ScholarPubMed
Christensen, G. D., Simpson, W. A. & Beachey, E. H. (1985). The adherence of staphylococci to smooth surfaces. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. 1, Abteilung (Suppl. 14), 471–475.Google Scholar
Christensen, G. D., Simpson, W. A., Younger, J. J., Baddour, L. M., Barrett, F. F., Melton, D. M. & Beachey, E. H. (1985). Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of Clinical Microbiology 22, 996–1006.CrossRefGoogle ScholarPubMed
Davenport, D. S., Massanari, R. M., Pfaller, M. A., Bale, M. J., Streed, S. A. & Hierholzer, W. J. (1986). Usefulness of a test for slime production as a marker for clinically significant infections with coagulase-negative staphylococci. The Journal of Infectious Diseases 153, 332–339.CrossRefGoogle ScholarPubMed
Davies, A. J., Clewett, J., Jones, A. & Marshall, R. (1986). Sensitivity patterns of coagulase-negative staphylococci from neonates. Journal of Antimicrobial Chemotherapy 17, 155–160.CrossRefGoogle ScholarPubMed
Del Bene, V. E., John, J. F., Twitty, J. A. & Lewis, J. W. (1986). Anti-staphylococcal activity of teicoplanin, vancomycin and other antimicrobial agents: the significance of methicillin resistance. The Journal of Infectious Diseases 154, 349–352.CrossRefGoogle ScholarPubMed
Diaz-Mitoma, F., Harding, G. K. M., Hoban, D. J., Roberts, R. S. & Low, D. E. (1987). Clinical significance of a test for slime production in ventriculoperitoneal shunt infections caused bycoagulase-negative staphylococci. The Journal of Infectious Diseases 156, 555–560.CrossRefGoogle ScholarPubMed
Dobson, A. J. (1983). An Introduction to Statistical Modelling. London: Chapman and Hall.CrossRefGoogle Scholar
Eng, R. H. K., Wang, C., Person, A., Kiehn, T. E. & Armstrong, D. (1982). Species identification of coagulase-negative staphylococcal isolates from blood cultures. Journal of Clinical Microbiology 15, 439–442.CrossRefGoogle ScholarPubMed
Eykyn, S. J. (1984). Infection and intravenous catheters. Journal of Antimicrobial Chemotherapy 14, 203–208.CrossRefGoogle ScholarPubMed
Gemmell, C. G. (1983). Extracellular toxins and enzymes of coagulase-negative staphylococci. In Staphylococci and Staphylococcal Infections, vol 11 (ed. Easmon, C. S. F. and Adlam, C.), pp. 809–827. London: Academic Press.Google Scholar
Gemmell, C. G. & Dawson, J. E. (1982). Identification of coagulase-negative staphylococci with the API Staph System. Journal of Clinical Microbiology 16, 874–877.CrossRefGoogle ScholarPubMed
Giger, O., Charilaou, C. C. & Cundy, K. R. (1984). Comparison of the API Staph-Ident and DMS Staph-Trac Systems with conventional methods used for the identification of coagulase-negative staphylococci. Journal of Clinical Microbiology 19, 68–72.CrossRefGoogle ScholarPubMed
Gill, V. J., Selepak, S. T. & Williams, E. C. (1983). Species identification and antibiotic susceptibilities of coagulase-negative staphylococci isolated from clinical specimens. Journal of Clinical Microbiology 18, 1314–1319.CrossRefGoogle ScholarPubMed
Goodfellow, M. (1987). Taxonomy of coagulase-negative staphylococci. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. 1. Abteilung (Suppl. 16), 1–14.Google Scholar
Gruer, L. D., Bartlett, R. & Ayliffe, G. A. J. (1984). Species identification and antibiotic sensitivity of coagulase-negative staphylococci from CAPD peritonitis. Journal of Antimicrobial Chemotherapy 13, 577–583.CrossRefGoogle ScholarPubMed
Hamilton-Miller, J. M. T. & Iliffe, A. (1985). Antimicrobial resistance in coagulase-negative staphylococci. Journal of Medical Microbiology 19, 217–226.CrossRefGoogle ScholarPubMed
Ishak, M. A., Gröschel, D. H. M., Mandell, G. L. & Wenzel, R. P. (1985). Association of slime with pathogenicity of coagulase-negative staphylococci causing nosocomial septicaemia. Journal of Clinical Microbiology 22, 1025–1029.CrossRefGoogle Scholar
Jaffe, H. W., Sweeney, H. M., Nathan, C., Weinstein, R. A., Kabins, S. A. & Cohen, S. (1980). Identity and interspecific transfer of gentamicin-resistance plasmids in Staphylococcus aureus and Staphylococcus epidermidis. The Journal of Infectious Diseases 141, 738–747.CrossRefGoogle ScholarPubMed
Kloos, W. E. (1986). Ecology of humanskin. In Coagulase Negative Staphylococci (ed. Mårdh, P. A. and Schleifer, K. H.), pp. 37–50. Sweden: Almqvist & Wiksell.Google Scholar
Kloos, W. E. & Musselwhite, M. S. (1975). Distribution and persistence of Staphylococcus and Micrococcus species and other aerobic bacteria on human skin. Applied Microbiology 30, 381–395.CrossRefGoogle ScholarPubMed
Kloos, W. E. & Schleifer, K. H. (1975). Simplified scheme for routine identification of human staphylococcus species. Journal of Clinical Microbiology 1, 82–88.CrossRefGoogle ScholarPubMed
Kristinsson, K. G., Spencer, R. C. & Brown, C. B. (1986). Clinical importance of production of slime by coagulase-negative staphylococci in chronic ambulatory peritoneal dialysis. Journal of Clinical Pathology 39, 117–118.CrossRefGoogle ScholarPubMed
Larson, E. L., McGinley, K. J., Foglia, A. R., Talbot, G. H. & Leyden, J. J. (1986). Composition and antimicrobic resistance of skin flora in hospitalized and healthy adults. Journal of Clinical Microbiology 23, 604–608.CrossRefGoogle ScholarPubMed
Lidwell, O. M., Lowbury, E. J. L., Whyte, W., Blowers, R., Stanley, S. J. & Lowe, D. (1983). Bacteria isolated from deep joint sepsis after operation for total hip or knee replacement and the sources of the infections with Staphylococcus aureus. Journal of Hospital Infection 4, 19–29.CrossRefGoogle ScholarPubMed
Maki, D. G., Weise, C. E. & Sarafin, H. W. (1977). A semiquantitative culture method for identifying intravenous-catheter-related infection. New England Journal of Medicine 296, 1305–1310.CrossRefGoogle ScholarPubMed
Marrie, T. J. & Costerton, J. W. (1984). Scanning and transmission electron microscopy of in situ bacterial colonization of intravenous and intraarterial catheters. Journal of Clinical Microbiology 19, 687–693.CrossRefGoogle ScholarPubMed
Marsik, F. J. & Brake, S. (1982). Species identification and susceptibility to 17 antibiotics of coagulase-negative staphylococci isolated from clinical specimens. Journal of Clinical Microbiology 15, 640–645.CrossRefGoogle ScholarPubMed
McAllister, T. A., Mocan, H., Murphy, A. V. & Beattie, T. J. (1987). Antibiotic susceptibility of staphylococci from CAPD peritonitis in children. Journal of Antimicrobial Chemotherapy 19, 95–100.CrossRefGoogle ScholarPubMed
McCullagh, P. & Nelder, J. A. (1983). Generalised Linear Models. London: Chapman and Hall.CrossRefGoogle Scholar
Mickelsen, P. A., Plorde, J. J., Gordon, K. P., Hargiss, C., McClure, J., Schoenknecht, F. D., Condie, F., Tenover, F. C. & Tompkins, L. S. (1985). Instability of antibiotic resistance in a strain of Staphylococcus epidermidis isolated from an outbreak of prosthetic valve endocarditis. The Journal of Infectious Diseases 152, 50–58.CrossRefGoogle Scholar
Naidoo, J. & Noble, W. C. (1987). Skin as a source of transferable antibiotic resistance in coagulase-negative staphylococci. Zentralblatt für Bakteriologie. Mikrobiologie und Hygiene. 1. Abteilung. (Suppl. 16), 225–234.Google Scholar
Needham, C. A. & Stempsey, W. (1984). Incidence, adherence, and antibiotic resistance of coagulase-negative staphylococcus species causing human disease. Diagnostic Microbiology and Infectious Disease 2, 293–299.CrossRefGoogle ScholarPubMed
Payne, C. D. (ed.) (1985). Generalised Linear Interactive Modelling. The GLIM System: release 3.77. Oxford: Royal Statistical Society, Numerical Algorithms Group.Google Scholar
Peters, G., Schumacher-Perdreau, F., Jansen, B., Bey, M. & Pulverer, G. (1987). Biology of S. epidermidis extracellular slime. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. 1. Abteilung. (Suppl. 16), 15–32.Google Scholar
Price, S. B. & Flournoy, D. J. (1982). Comparison of antimicrobial susceptibility patterns among coagulase negative staphylococci. Antimicrobial Agents and Chemotherapy 21, 436–440.CrossRefGoogle ScholarPubMed
Reuther, J. W. (1986). A simplified system for the identification of staphylococci by multipoint inoculation of test media. Journal of Medical Microbiology 22, 179–182.CrossRefGoogle ScholarPubMed
Richardson, J. F. & Marples, R. R. (1982). Changing resistance to antimicrobial drugs and resistance typing in clinically-significant strains of Staphylococcus epidermidis. Journal of Medical Microbiology 15, 475–484.CrossRefGoogle ScholarPubMed
Richardson, J. F., Marples, R. R. & De Saxe, M. J. (1984). Characters of coagulase-negative staphylococci and micrococci from cases of endocarditis. Journal of Hospital Infection 5, 164–171.CrossRefGoogle ScholarPubMed
Schleifer, K. H. & Kloos, W. E. (1975). A simple test system for the separation of staphylococci from micrococci. Journal of Clinical Microbiology 1, 337–338.CrossRefGoogle ScholarPubMed
Sewell, C. M., Clarridge, J. E., Young, E. J. & Guthrie, R. (1982). Clinical significance of coagulase-negative staphylococci. Journal of Clinical Microbiology 16, 236–239.CrossRefGoogle ScholarPubMed
Varaldo, P. E., Cipriani, P., Focá, A., Geraci, C., Giordano, A., Madeddu, M. A., Orsi, A., Pompei, R., Prenna, M., Repetto, A., Ripa, S., Rosselli, P., Russo, G., Scazzocchio, F. & Stassi, G. (1984). Identification, clinical distribution, and susceptibility to methicillin and 18 additional antibiotics of clinical Staphylococcus isolates: nationwide investigation in Italy. Journal of Clinical Microbiology 19, 838–843.CrossRefGoogle ScholarPubMed
Wade, J. C., Schimpff, S. C., Newman, K. A. & Wiernik, P. H. (1982). Staphylococcus epidermidis: an increasing cause of infection in patients with granulocytopenia. Annals of Internal Medicine 97, 503–508.CrossRefGoogle ScholarPubMed
West, T. E., Walshe, J. J., Krol, C. P. & Amsterdam, D. (1986). Staphylococcal peritonitis in patients on continous peritoneal dialysis. Journal of Clinical Microbiology 23, 809–812.CrossRefGoogle Scholar
Younger, J. J., Christensen, G. D., Bartley, D. L., Simmons, J. C. H. & Barrett, F. F. (1987). Coagulase-negative staphylococci isolated from cerebrospinal shunts: importance of slime production, species identification and shunt removal to clinical outcome. The Journal of Infectious Diseases 156, 548–554.CrossRefGoogle ScholarPubMed
Zeirdt, C. H. & Golde, D. W. (1970). Deoxyribonuclease-positive Staphylococcus epidermidis strains. Applied Microbiology 20, 54–57.CrossRefGoogle Scholar