Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-28T23:01:24.086Z Has data issue: false hasContentIssue false
  • English
  • Français

Some factors affecting the viability of freeze-thawed T4 bacteriophage

Published online by Cambridge University Press:  15 May 2009

P. R. M. Steele ,
J. D. Davies  and
R. I. N. Greaves
P. R. M. Steele
Affiliation:
The University Department of Pathology, Cambridge, England
J. D. Davies
Affiliation:
The University Department of Pathology, Cambridge, England
R. I. N. Greaves
Affiliation:
The University Department of Pathology, Cambridge, England
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Some effects of freeze-thawing on the T 4 bacteriophage have been studied. The results indicated that most of the inactivation of rapidly thawed samples was due to osmotic damage, whereas inactivation of slowly thawed samples appeared to be correlated with the eutectic temperature of the suspending medium. Peptone significantly increased the survival of slowly thawed T 4 phage. The particular constituents of peptone largely responsible for this protection were separated as two distinct peptide fractions, using G 25 Sephadex.

This work has been supported in part by grants from the Medical Research Council and the Office of Naval Research under contract F61052–68-C-0041.

Type
Research Article
Information
Journal of Hygiene , Volume 67 , Issue 1 , March 1969 , pp. 107 - 114
Copyright
Copyright © Cambridge University Press 1969

References

Adams, M. (1959). Bacteriophages. New York: Wiley.CrossRefGoogle Scholar
Anderson, T. F. (1953). The morphology and osmotic properties of bacteriophage systems. Cold Spring Harb. Symp. quant. Biol. 18, 197–206.CrossRefGoogle ScholarPubMed
Greaves, R. I. N. & Davies, J. D. (1965). Separate effects of freezing, thawing and drying living cells. Ann. N.Y. Acad. Sci. 125, 548–58.CrossRefGoogle ScholarPubMed
Greaves, R. I. N., Davies, J. D. & Steele, P. R. M. (1967). The freeze-drying of frost-sensitive organisms. Cryobiology 3, 283–7.Google Scholar
Leibo, S. B. & Mazur, P. (1967). Freezing of bacteriophage T4B. Cryobiology 3, 366–7.Google Scholar
Schito, G. C. (1967). A rapid purification procedure for bacterial viruses. Virology 30, 305–7.Google Scholar
VanDen Berg, L. Den Berg, L. (1959). Effect of additions of NaC1 and KC1 on the system of KH2PO4Na2HPO4-H2O on pH and composition during freezing. Archs Biochem. Biophys. 34, 305–15.Google Scholar