Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-22T15:33:33.924Z Has data issue: false hasContentIssue false
  • English
  • Français

Some factors affecting preservability of freeze-dried bacteria

Published online by Cambridge University Press:  15 May 2009

Yoji Obayashi ,
Sunao Ota  and
Shiro Arai
Yoji Obayashi
Affiliation:
Research Institute, Japan Anti-Tuberculosis Association, Tokyo, Japan
Sunao Ota
Affiliation:
Research Institute, Japan Anti-Tuberculosis Association, Tokyo, Japan
Shiro Arai
Affiliation:
Research Institute, Japan Anti-Tuberculosis Association, Tokyo, Japan
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The use of sodium glutamate as a medium for freeze-drying Lact. bifidus significantly enhanced the stability of the dried product, especially the heat stability.

2. The optimal concentration of sodium glutamate, as a drying medium, was found to depend on the bacilliary concentration; the higher the concentration of the bacilliary suspension used the higher was found to be the optimal concentration of the sodium glutamate.

3. It was possible to classify various drying media according to the degree of sublimation of the product during the process of freeze-drying. Two types were observed; in the first, which included sodium glutamate and glucose, the sublimation rate diminished with the increasing concentration of the medium, whereas in the second group, which included polyvinyl pyrrolidon K 90 (PVP), soluble starch and dextran, no such diminution was observed when the concentration of the medium was increased.

4. By the combination of sodium glutamate or glucose with a medium belonging to the second group sublimation was found to be promoted.

5. The stabifity-conferring effect of sodium glutamate on dried Lact. bifidus was markedly enhanced when the glutamate was combined with a drying medium belonging to the second group. This effect is attributed to a desiccation-promoting property of the latter medium.

6. Lact. bifidus as a freeze-dried preparation showed a somewhat better survival rate when sealed in air than when sealed in vacuo. In the case of other anaerobic organisms tested there was no significant difference in the survival rates under the two methods of storage.

Type
Research Article
Information
Journal of Hygiene , Volume 59 , Issue 1 , March 1961 , pp. 77 - 91
Copyright
Copyright © Cambridge University Press 1961

References

REFERENCES

Bergey, D. H.Manual of Determinative Bacteriology. (1957). 7th ed.Baltimore: The Williams and Wilkins Co.Google Scholar
Cho, C. & Obayashi, Y. (1956). Effect of adjuvant on preservability of dried B.C.G. vaccine at 37° C. Bull. World Hlth Org. 14, 657.Google Scholar
Dehnert, J. (1957). Untersuchung über die gram-positive Stuhlflora des Brustmilchkindes. Zbl. Bakt. (1. Abt. Orig.), 169, 66.Google Scholar
Greaves, R. I. N. (1960). Some factors which influence the stability of freeze-dried cultures. Recent Research in Freezing and Drying, p. 203. Oxford: Blackwell Scientific Publications.Google Scholar
Miller, R. Jr. & Goodner, K. (1953). Studies on the stability of lyophilized B.C.G. vaccine. Yale J. Biol. Med. 25, 262.Google ScholarPubMed
Muggleton, P. W. (1960). Freeze-drying of bacteria with special reference to B.C.G. Recent Research in Freezing and Drying, p. 229. Oxford: Blackwell Scientific Publications.Google Scholar
Obayashi, Y. (1955). Dried B.C.G. vaccine. p. 23. World Monogr. Ser. no. 28. Geneva: W.H.O.Google Scholar
Obayashi, Y. (1960). The preservation of B.C.G. Recent Research in Freezing and Drying, p. 221. Oxford: Blackwell Scientific Publications.Google Scholar
Obayashi, Y. & Cho, C. (1957). Further studies on the adjuvant for dried B.C.G. vaccine. Bull. World Hlth Org. 17, 255.Google Scholar
Ota, S. (1959). Studies on freezing and freeze-drying of Lactobacillus bifidus (in Japanese). Ochanomizu Igaku Zassi, 7, 2954.Google Scholar
Scott, W. J. (1960). A mechanism causing death during storage of dried microorganisms. Recent Research in Freezing and Drying, p. 188. Oxford: Blackwell Scientific Publications.Google Scholar
Yanagisawa, K., Kitaoka, M., Tagaya, I., Soekawa, M., Yamanouchi, K., Sawada, T. & Suzuki, M. (1960). Studies on the lyophilization of smallpox vaccine. Report to the Second Conference on Freezing and Drying.July 1960.Japan:Sapporo.Google Scholar