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Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody

Published online by Cambridge University Press:  15 May 2009

J. R. Crowther ,
R. C. Wardley  and
P. J. Wilkinson
J. R. Crowther
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey, U.K.
R. C. Wardley
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey, U.K.
P. J. Wilkinson
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey, U.K.
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Summary

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A solid phase radioimmunoassay (RIA) has been successfully developed to measure both African swine fever virus (ASFV) antigen and antibody. Studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50–500 HAD50/ml. Both direct and indirect antibody RIA have been developed and have proved to be approximately 100 times more sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophoresis test for the detection of ASFV antibody.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 2 , October 1979 , pp. 353 - 361
Copyright
Copyright © Cambridge University Press 1979

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