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A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody

Published online by Cambridge University Press:  15 May 2009

R. C. Wardley ,
E. M. E. Abu Elzein ,
J. R. Crowther  and
P. J. Wilkinson
R. C. Wardley
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
E. M. E. Abu Elzein
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
J. R. Crowther
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
P. J. Wilkinson
Affiliation:
The Animal Virus Research Institute, Pirbright, Surrey GU24 0NF, England
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Summary

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A solid-phase enzyme-linked immunosorbent assay was developed to measure both African swine fever virus (ASFV) antigen and antibody. Experiments showed it to be reproducible and able to detect limiting antigen concentrations of 50–500 HAD50/ml. The assay was more sensitive than those used at present to detect ASFV antibody and it is suggested that it might be of great diagnostic use in countries where African swine fever has recently appeared.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 2 , October 1979 , pp. 363 - 370
Copyright
Copyright © Cambridge University Press 1979

References

Abu Elzein, E. M. E. & Crowther, J. R. (1978). Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research. Journal of Hygiene 80, 391–9.CrossRefGoogle ScholarPubMed
Akao, Y., Sano, R., Sagiwa, A. & Shimojo, H. (1962). Procomplementary activity of swine serum and its elimination with periodate treatment. Japanese Journal of Medical Science and Biology 15, 165–73.CrossRefGoogle ScholarPubMed
Avrameas, S. (1969). Coupling of enzymes to proteins with glutaraldehyde. Immunochemistry 6, 43–52.CrossRefGoogle ScholarPubMed
Crowther, J. R., Wardley, R. C. & Wilkinson, P. J. (1979). Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody. Journal of Hygiene 83, 353–61.CrossRefGoogle ScholarPubMed
Malmquist, W. A. & Hay, D. (1960). Haemadsorption and cytopathic effect produced by African swine fever virus in swine bone marrow and buffy coat cultures. American Journal of Veterinary Research 21, 104–8.Google Scholar
Pan, I. C., Deboer, C. J. & Hess, W. R. (1972). African swine fever: application of immuno electro osmophoresis for the detection of antibody. Canadian Journal of Comparative Medicine 36, 309–16.Google Scholar
Voller, A. & Bidwell, D. E. (1976). Enzyme immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. British Journal of Experimental Pathology 57, 243–7.Google ScholarPubMed
Voller, A., Bidwell, D. E. & Bartlett, A. (1976). Microplate enzyme immunoassays for the immunodiagnosis of virus infections. Manual of Clinical Immunology 69, pp. 506–12. American Society of Microbiology, Washington.Google Scholar