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Sodium Diethyldithiocarbamate and Oxine in the Differentiation of Brucella Species

Published online by Cambridge University Press:  15 May 2009

J. C. Cruickshank
J. C. Cruickshank
Affiliation:
London School of Hygiene and Tropical Medicine
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The effect of two chelating agents, sodium diethyldithiocarbamate and 8-hydroxy-quinoline (oxine), on 70 strains of brucellae (24 Br. abortus, 22 Br. melitensis and 28 Br. suis) has been examined.

The pattern of growth and inhibition of the three species as it appears in the carbamate test described by Renoux is of some value in the differentiation of species. Br. suis is more resistant to carbamate than the other two species.

The three species also differ in their sensitivity to oxine, and strains of Br. suis from various sources were consistently more resistant than the other species. This observation may be of use in the identification of Br. suis.

My thanks are due to Prof. Adrian Albert and Dr J. H. Marshall for helpful discussion of the chemical aspects of this work, and to Mr B. Madge, A.I.M.L.T., for technical assistance.

Type
Research Article
Information
Journal of Hygiene , Volume 53 , Issue 3 , September 1955 , pp. 305 - 312
Copyright
Copyright © Cambridge University Press 1955

References

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