Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-22T15:16:10.640Z Has data issue: false hasContentIssue false
  • English
  • Français

The site of specific substances in capsulated organisms

Published online by Cambridge University Press:  15 May 2009

Cella Barber
Cella Barber
Affiliation:
Department of General Biochemistry, ‘Dr I. Cantacuzino’ Institute, Bucharest
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Applying a method of selective separation of the specific high polymers the intracellular site of the specific substance in the Copenhagen strain of Pneumococcus Type II was demonstrated, by electron microscopy; the specific polymeric material assumed to form the capsule is situated within the bacterial cell and the electron density of this material is sufficiently high to permit its detection.

The possibility of obtaining electron micrographs of the polymeric substances as contained in the cells or after their diffusion into the medium or when secreted as slime, suggests that the current concept of the capsules as the site of the specific substances may have to be reviewed.

The electron micrographs were made by Dr A. Petrovici to whom I express my thanks.

Type
Research Article
Information
Journal of Hygiene , Volume 62 , Issue 1 , March 1964 , pp. 121 - 126
Copyright
Copyright © Cambridge University Press 1964

References

REFERENCES

Barber, C., Stamatescu, S. & Wisner, B. (1958). Recherches sur les antigènes capsulaires et somatiques des Klebsiella. J. Hyg. Epidem. (Praha), 2, 57.Google ScholarPubMed
Barber, C., Baldovin-Agapt, C., Beloiu, I. & Caffé, I. (1959). Sur les antigènes spécifiques de type des Pneumocoques. Arch. roum. Path. exp. Microbiol., 18, 287.Google Scholar
Barber, C., Petrovici, A., Zilisteaunu, E. & Nafta, I. (1960). Recherches sur les haptènes du Bacille Anthracis et leur localisation dans Ia cellule bactérienne. Arch. roum. Path. exp. Microbiol. 19, 379.Google Scholar
Barber, C., Stamatescu-Eustatziou, S., Tulpan, G. & Petrovici, A. (1960). On the specific (somatic) polysaccharides of Klebsiella. J. Hyg. Epidem. (Praha), 4, 387.Google ScholarPubMed
Barber, C., Eustatziou, S. & Andreica, A. (1961). Contribution à l'étude de la biologie des Klebsiella. III. Étude chimique et immunologique des polysaccharides isolés de deux souches capsulées et de leurs variantes non-capsulées. Arch. roum. Path. exp. Microbiol. 20, 103.Google Scholar
Barber, C. & Taga, S. (1964). Contribution à l'étude antigénique des Staphilocoques. Séparation d'antigènes à partir de deux Staphilocoques et de leurs mutants capsulés. Arch. roum. Path. exp. Microbiol. in the Press.Google Scholar
Brieger, E. M. (1963). Structure and Ultrastructure of Micro-organisms. (Capsule formation, p. 273.) New York and London: Academic Press, Inc.Google Scholar
Ciuca, M., Stamatescu-Eustatziou, S., Barber, C., Voinea, M. & Tulpan, G. (1959). Contribution à l'étude de Ia biologie des Klebsiella. I. Étude biologique et immunochimique de certaines souches de Klebsiella. Arch. roum. Path. exp. Microbiol. 18, 347.Google Scholar
Eustatziou, S., Barber, C., Voinea, M. & Tulpan, G. (1960). Contribution à l'étude de Ia biologie des Klebsiella. II. Étude biologique et immunochimique de deux variantes ‘S’ de Klebsiella. Arch. roum. Path. exp. Microbiol. 19, 367.Google Scholar
Goslings, W. R. O. (1935). Untersuchungen über das Scleroma respiratorium. III. Mitteilung: Die Variabilität bei Kapselbakterien, besonders bei Sklerombakterien. Zbl. Bakt. (1. Abt. Orig.), 134, 195.Google Scholar
Knaysi, G. (1950). Cited by Salton, M. R. (1960).The Bacteria. Vol. i. Structure. (Surface layers of the bacterial cell, p. 97.) Ed. Gunzalus, I. C and R, Stanier.New York and London: Academic Press, Inc.Google Scholar
Lenhart, N., Li, I. W., DeCourcy, S. J. Jr, & Mudd, S. (1962). Non-mucoid mutant of the encapsulated Smith strain of Staphylococcus aureus. J. Bact. 83, 1165.CrossRefGoogle Scholar
Price, K. M. & Kneeland, Y. (1956). Further studies of the phenomenon of capsular swelling of Micrococcus pyogenes var. aureus in the presence of immune serum. J. Bact. 71, 229.CrossRefGoogle ScholarPubMed
Read, B. E., Keller, R. & Cabelli, V. J. (1957). The decapsulation phenomenon of Klebsiella pneumoniae. J. Bact. 73, 765.CrossRefGoogle ScholarPubMed
Sahab, K. (1960). Method for demonstrating capsules of Enterobacteriaceae. J. Bact. 79, 198.CrossRefGoogle ScholarPubMed
Salton, M. R. (1960). The Bacteria. Vol. i. Structure. (Surface layers of the bacterial cell, p. 97.) Ed. Gunzalus, I. C and R, Stanier.New York and London: Academic Press Inc.Google Scholar
Salton, M. R. (1961). The anatomy of the bacterial surface. Bact. Rev. 25, 77.CrossRefGoogle ScholarPubMed
Smith, C. W. & Metzger, J. F. (1962). Demonstration of a capsular structure on Listeria monocytogenes. Path. et Microbiol. (Basel), 25, 499.Google ScholarPubMed
Tomcsik, J. (1956). Bacterial Anatomy. The Sixth Symposium of the Society for General Microbiology. (Bacterial capsules and their relation to the cell wall (p. 41) and Specific capsular reaction: chemical nature of the capsule (p. 48).) Ed. Spooner, E. T. C and Stocker, B. A. D.Cambridge University Press.Google Scholar
Wadsworth, A. & Brown, R. (1933). Chemical and immunological studies of the pneumococcus. III. Cellular carbohydrate fractions. J. Immunol. 24, 349.CrossRefGoogle Scholar
Winne, E. S. & Volkman, C. M. (1963). An unusually deceptive staining artefact. Bact. Proc. G. 94, p. 47.Google Scholar