Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-19T05:22:56.220Z Has data issue: false hasContentIssue false
  • English
  • Français

A simplified method for the detection of rubella-specific IgM employing sucrose density fractionation and 2-mercaptoethanol

Published online by Cambridge University Press:  15 May 2009

E. O. Caul ,
G. W. Smyth  and
Suzanne K. R. Clarke
E. O. Caul
Affiliation:
Public Health Laboratory, Myrtle Road, Kingsdown, Bristol, BS2 8EL
G. W. Smyth
Affiliation:
Public Health Laboratory, Myrtle Road, Kingsdown, Bristol, BS2 8EL
Suzanne K. R. Clarke
Affiliation:
Public Health Laboratory, Myrtle Road, Kingsdown, Bristol, BS2 8EL
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A simplified method for the detection of rubella-specific IgM in sera was developed involving HAI tests on only one fraction from a sucrose density gradient with and without treatment with 2-mercaptoethanol (2 ME). Slight trailing of IgG into the IgM fraction was shown to occur in patients with high titres of both IgG and IgM, but this could be detected by the 2ME treatment and did not affect the results.

By this test, rubella-specific IgM was found in all of 54 patients with a rash and a rise of rubella HAI antibodies. When the antigen and serum were incubated for 1 hr. before adding the RBC, specific IgM was found in all 51 sera taken between 5 and 40 days after onset, and in about half the sera taken between 42 and 77 days, but in none of 16 people known to have possessed rubella antibodies at least a year previously. When antigen and serum were incubated for 18 hr. before adding the RBC, specific IgM was found in all of 21 sera taken between 2 and 49 days after onset, and in 6 of 11 sera taken between 50 and 117 days, but not in 9 people known to have possessed rubella antibodies at least a year before. The method has been found to be very useful in detecting recent rubella among patients in early pregnancy.

Type
Research Article
Information
Journal of Hygiene , Volume 73 , Issue 3 , December 1974 , pp. 329 - 340
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Banatvala, J. E., Best, J. M., Kennedy, E. A., Smith, E. E. & Spence, M. E. (1967). A serological method for demonstrating recent infection by rubella virus. British Medical Journal iii, 285.CrossRefGoogle Scholar
Baublis, J. V. & Brown, G. C. (1968). Specific response of the immunoglobulins to rubella infections. Proceedings of the Society for Experimental Biology and Medicine 128, 206.CrossRefGoogle Scholar
Best, J. & Banatvala, J. E. (1969). Serological tests for rubella. Lancet ii, 546.CrossRefGoogle Scholar
Best, J. M., Banatvala, J. E. & Watson, D. (1969). Serum IgM and IgG responses in postnatally acquired rubella. Lancet ii, 65.CrossRefGoogle Scholar
Bürgin-Wolff, A., Hernandez, R. & Just, M. (1971). Separation of rubella IgM, IgA and IgG antibodies by gel filtration on agarose. Lancet ii, 1278.CrossRefGoogle Scholar
Cohen, S. M., Ducharme, C. P., Carpenter, C. A. & Deibel, R. (1968). Rubella antibody in IgG and IgM immunoglobulins detected by immunofluorescence. Journal of Laboratory and Clinical Medicine 72, 760.Google ScholarPubMed
Cradock-Watson, J. E., Bourne, M. S. & Vandervelde, E. M. (1972). IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique. Journal of Hygiene 70, 473.CrossRefGoogle ScholarPubMed
Cradock-Watson, J. E., Ridehalgh, M. K. S., Bourne, M. S. & Vandervelde, E. M. (1973). Nasal immunoglobulin responses in acute rubella determined by the immunofluorescent technique. Journal of Hygiene 71, 603.CrossRefGoogle ScholarPubMed
Desmyter, J. (1972). Absence of non-acute rubella IgM antibodies in chronic hepatitis and rheumatoid arthritis. Lancet ii, 1141.CrossRefGoogle Scholar
Desmyter, J., South, M. A. & Rawls, W. E. (1971). The IgM antibody response in rubella during pregnancy. Journal of Medical Microbiology 4, 107.CrossRefGoogle ScholarPubMed
Field, P. R. & Murphy, A. M. (1972). The role of specific IgM globulin estimations in the diagnosis of acquired rubella. Medical Journal of Australia 2, 1244.CrossRefGoogle ScholarPubMed
Forghani, B., Schmidt, N. J. & Lennette, E. H. (1973). Demonstration of rubella IgM antibody by indirect fluorescent antibody staining, sucrose density gradient centrifugation and mercaptoethanol reduction. Intervirology 1, 48.CrossRefGoogle ScholarPubMed
Fraser, K. B., Shirodaria, P. V. & Stanford, C. F. (1971). Fluorescent staining and human IgM. British Medical Journal iii, 707.CrossRefGoogle Scholar
Gupta, J. D., Peterson, V., Stout, M. & Murphy, A. M. (1971). Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column. Journal of Clinical Pathology 24, 547.CrossRefGoogle ScholarPubMed
Gupta, J. D., Peterson, V. J. & Murphy, A. M. (1972). Differential immune response to attenuated rubella virus vaccine. Infection and Immunity 5, 151.CrossRefGoogle ScholarPubMed
Haire, M. & Hadden, D. S. M. (1970). Immunoglobulin responses in rubella and its complications. British Medical Journal iii, 130.CrossRefGoogle Scholar
Haire, M. & Hadden, D. S. M. (1972). Rapid diagnosis of rubella by demonstrating rubella-specific IgM antibodies in the serum by indirect immunofluorescence. Journal of Medical Microbiology 5, 237.CrossRefGoogle ScholarPubMed
Haire, M., Adair, B. McC. & Fraser, K. B. (1972). The effect of temperature and time of incubation in the preparation of antigens for detection of virus-specific IgM antibody. Clinical and Experimental Immunology 13, 327.Google Scholar
Newman, S., Horta-Barbosa, L. & Sever, J. L. (1969). Serological tests for rubella. Lancet ii, 432.CrossRefGoogle Scholar
Ogra, P. L., Kerr-Grant, D., Umana, G., Dzierba, J. & Weintraub, D. (1971). Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus. New England Journal of Medicine 285, 1333.CrossRefGoogle ScholarPubMed
Pattison, J. R. & Mace, J. E. (1973). A simple, inexpensive gel filtration technique for use in diagnostic serology. Journal of Clinical Pathology 26, 309.CrossRefGoogle ScholarPubMed
Pead, P. J. (1974). Estimation of rubella-specific IgM antibody in sera by single fraction collection on Sephadex G-200. Medical Laboratory Technology 31, 159.Google ScholarPubMed
Sever, J. L. (1969). Immunoglobulin determinations for the detection of perinatal infections. Journal of Pediatrics 75, 1111.CrossRefGoogle ScholarPubMed
Vesikari, T. & Vaheri, A. (1968). Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies. British Medical Journal i, 221.CrossRefGoogle Scholar