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A simple modification of Wilson and Blair's medium for the isolation of typhoid and paratyphoid bacilli

(Brilliant green, bismuth-ammonium-citrate and sodium sulphite medium—B.B.S. medium)

Published online by Cambridge University Press:  15 May 2009

Saga Tyagaraja
Saga Tyagaraja
Affiliation:
From the City Microbiological Laboratory, Colombo
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A simple modification of Wilson & Blair's medium for the isolation of typhoid and paratyphoid bacilli has been evolved.

1. Instead of the original six ingredients, this modification contains only three, namely, brilliant green, bismuth-ammonium-citrate and sodium sulphite, and may therefore be conveniently termed “B.B.S. medium”.

2. The glucose, sodium phosphate and ferrous sulphate of the original medium are eliminated. The bismuth-ammonium-citrate solution is made without caustic soda.

3. The B.B.S. medium keeps well for several weeks. Deterioration can easily be detected and readily rectified.

4. The inhibitory action towards B. aerogenes is greatly improved in the B.B.S. medium. This is particularly advantageous in countries where this organism is frequently encountered.

5. B. typhosus and B. paratyphosus grow readily. The colonies are sufficiently characteristic and can be picked out early for confirmatory tests.

6. Using B.B.S. medium a complete diagnosis should be possible within 48 hr.

Type
Research Article
Information
Journal of Hygiene , Volume 40 , Issue 4 , July 1940 , pp. 414 - 422
Copyright
Copyright © Cambridge University Press 1940

References

REFERENCES

Tabet, F. (1938). Modification of Wilson and Blair's bismuth medium suitable for both typhoid and paratyphoid bacilli. J. Path. Bact. 46, 181–93.CrossRefGoogle Scholar
Wilson, W. J. (1938). Isolation of Bact. typhosum by means of bismuth sulphite medium in water- and milk-borne epidemics. J. Hyg., Camb., 38, 507–19.Google ScholarPubMed
Wilson, W. J. & Blair, E. M. McV. (1927). Use of a glucose bismuth sulphite iron medium for the isolation of B. typhosus and B. proteus. J. Hyg., Camb., 26, 374–91.Google ScholarPubMed
Wilson, W. J. & Blair, E. M. McV. (1931). Further experience of the bismuth sulphite media in the isolation of Bacillus typhosus and B. paratyphosus from faeces, sewage and water. J. Hyg., Camb., 31, 139–61.CrossRefGoogle Scholar