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A simple method for the determination of bacterial sensitivity to sulphonamides by the use of blotting-paper disks

Published online by Cambridge University Press:  15 May 2009

R. J. Evans
R. J. Evans
Affiliation:
From the Pathology Department, St Bartholomew's Hospital, London, E.C. 1
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A description is given of a simple method of determining the sensitivity of bacteria to sulphonamides by means of impregnated blotting-paper disks applied to nutrient agar prepared from broth freed from sulphonamide antagonists by incubation with lysed horse red blood cells.

With organisms sensitive to the selected sulphonamide, a zone of inhibition of growth will occur, the diameter of which is related to the concentration of the sulphonamide necessary to inhibit growth. Sensitivity to several sulphonamides can be determined simultaneously.

The impregnated disks can be stored without loss of potency.

Type
Research Article
Information
Journal of Hygiene , Volume 46 , Issue 4 , December 1948 , pp. 422 - 425
Copyright
Copyright © Cambridge University Press 1948

References

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