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Shiga toxin genes (stx) in Norwegian sheep herds

Published online by Cambridge University Press:  28 August 2001

A. M. URDAHL
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, P O Box 8146 Dep., N-0033 Oslo, Norway
O. ALVSEIKE
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, P O Box 8146 Dep., N-0033 Oslo, Norway
E. SKJERVE
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, P O Box 8146 Dep., N-0033 Oslo, Norway
Y. WASTESON
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, P O Box 8146 Dep., N-0033 Oslo, Norway
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Abstract

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The aim of this study was to describe the occurrence of shiga toxic genes (stx) in Norwegian sheep herds, and to identify herd management factors related to the occurrence of stx in herds. Faecal samples from 124 sheep-herds were collected at abattoirs in 1998. Pooled samples from lambs and from ewes were screened for stx by a PCR method directly on faeces. Of the 124 herds, 61 were positive for stx, giving an overall herd-prevalence of 49%. Twenty-one of the 61 positive herds were positive both in lamb and ewe samples, 24 only in lamb samples and 16 only in ewe samples. There was no difference in prevalence between regions. From the 21 herds positive both in lamb and ewe samples, stx encoding E. coli were detected in 18 herds using hydrophobic grid membrane filters and subsequent colony hybridization. Information about management factors was collected by telephone interviews. Having cattle at the same farm turned out to be a possible risk factor, with an Odds Ratio of 9·9 (CI 1·2 → ∞).

Type
Research Article
Copyright
2001 Cambridge University Press