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Serological typing of coagulase-negative staphylococci using monoclonal antibodies

Published online by Cambridge University Press:  15 May 2009

C. Gabelish ,
C. Harbour ,
M. A. Beard-Pegler ,
E. Stubbs ,
R. Steffe ,
M. Large ,
A. Vickery  and
R. Benn
C. Gabelish
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
C. Harbour*
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
M. A. Beard-Pegler
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
E. Stubbs
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
R. Steffe
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
M. Large
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
A. Vickery
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
R. Benn
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
*
Correspondence: Dr C. Harbour, Department of Infectious Diseases, University of Sydney, NSW 2006, Australia.
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Summary

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This investigation was to determine whether monoclonal antibodies (Mabs) could be used to differentiate coagulase-negative staphylococci (C-NS) at species and strain level. Mabs were produced to four Staphylococcus epidermidis strains, two S. haemolyticus strains, one S. saprophyticus strain and one S. warneri strain. A panel of nine antibodies was tested for species and strain specificity against five type strains and 65 clinical isolates of C-NS by enzyme-linked immunosorbent assay (ELISA). Species specificity was found with Mab D150 produced to one S. haemolyticus strain. Using Mab D150 and Mab D198 in conjunction, identification of 90% of S. haemolyticus isolates to species level was achieved. S. saprophyticus Mab K84 reacted with most other strains of C-NS tested but only three S. haemolyticus strains (16%). This finding provides further evidence that S. haemolyticus possesses different surface determinants to other C-NS which could form the basis of a typing scheme for S. haemolyticus using Mabs D150, D198 and K84.

Type
Special Article
Information
Epidemiology & Infection , Volume 106 , Issue 2 , April 1991 , pp. 231 - 237
Copyright
Copyright © Cambridge University Press 1991

References

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