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Serological tests for the detection of antibodies to Mycoplasma gallisepticum in chickens and turkeys

Published online by Cambridge University Press:  15 May 2009

F. T. W. Jordan  and
P. Kulasegaram
F. T. W. Jordan
Affiliation:
Poultry Section, Department of Veterinary Preventive Medicine, University of Liverpool Veterinary Field Station, Neston, Wirral, Cheshire, England
P. Kulasegaram
Affiliation:
Poultry Section, Department of Veterinary Preventive Medicine, University of Liverpool Veterinary Field Station, Neston, Wirral, Cheshire, England
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Summary

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A comparison was undertaken of several serological tests in determining the response of chickens and turkeys experimentally infected with the A 514 strain of Mycoplasma gallisepticum.

After a single intratracheal inoculation of chickens with a culture of the organism, the highest titres were obtained by the indirect complement fixation (ICF) test, followed by the tube agglutination (TA), haemagglutination inhibition (HI), slide agglutination (SA) and metabolic inhibition (MI) tests. By all these tests positive titres were observed within the first week and peak titres between the first and second weeks. At 5 months there was no positive reaction by the ICF test but most chickens gave positive readings by the TA, HI and SA tests for at least 14 months after infection, but turkey sera became negative by all tests after 3 months.

A disadvantage of the ICF test was that sera up to a dilution of 1/8 and 1/16 for chicken and turkey respectively were anticomplementary, and in turkeys this masked the ICF titre, which presumably was low following one intratracheal inoculation. Titres in turkeys with the TA, HI and SA tests followed the pattern seen with chickens and were generally lower than those found by other workers probably because of the avirulent nature of the inoculum used.

The WB test was the least sensitive of the agglutination tests but is useful as a flock test which can be undertaken on the farm.

The MI test gave the lowest titres of all and antibodies could be detected for only 4 months following one intratracheal inoculation. Even with serum prepared by multiple inoculations in chickens the titre was never higher than 1/32 compared with 1/1024 for serum similarly prepared in rabbits.

Precipitins were detected by the agar gel method in the sera of chickens and turkeys after two intratracheal inoculations but in only some of the chickens and none of the turkeys after one inoculation.

By all tests higher titres were observed with chicken than turkey sera and antibodies persisted for a longer time.

Re-infection of chickens when antibodies to the initial infection had become low, and of turkeys when antibodies were no longer detectable, gave rise to an anamnestic response with titres which were higher than before.

Antiserum to M. gallisepticum prepared in chickens is comparable with that prepared in rabbits except for low titres by the MI test.

Type
Original Articles
Information
Journal of Hygiene , Volume 66 , Issue 2 , June 1968 , pp. 249 - 267
Copyright
Copyright © Cambridge University Press 1968

References

Adler, H. E. (1954). A rapid slide agglutination test for the diagnosis of chronic respiratory disease in the field and laboratory infected chickens and turkeys—a preliminary report. Proc. Am. vet. med. Ass. Ann. Meeting, p. 346.Google Scholar
Adler, H. E. (1958). A PPLO slide agglutination test for the detection of infectious sinusitis of turkeys. Poult. Sci. 37, 1116.CrossRefGoogle Scholar
Adler, H. E. & DaMassa, A. J. (1964). Enhancement of mycoplasma agglutination titres by use of anti-globulin. Proc. Soc. exp. Biol. Med. 116, 608.CrossRefGoogle ScholarPubMed
Adler, H. E., Shifrine, M. & Ortmayer, H. (1962). Interpretation of Mycoplasma gallisepticum serological tests for turkeys. XIIth Wld's Poult. Congr. p. 322.Google Scholar
Aftosmis, T. G., Tourtellotte, M. E. & Jacobs, R. E. (1960). A sensitive whole blood test for Mycoplasma gallisepticum. Avian Dis. 4, 485.CrossRefGoogle Scholar
Baharsefat, M. & Adler, H. E. (1965). Comparison of tube agglutination, haemagglutination-inhibition (HI) and antiglobulin titres on sera of chickens and turkeys infected with Mycoplasma gallisepticum. Avian Dis. 9, 460.CrossRefGoogle Scholar
Boulanger, P. & Rice, C. E. (1953). A study of complement fixation methods as applied to the demonstration of antibodies in birds. Proc. Am. vet. med. Ass., Ann. Meeting, p. 316.Google Scholar
Crawley, J. F. (1960). Use of the hemagglutination-inhibition test in the control of chronic respiratory disease of chickens. Ann. N.Y. Acad. Sci. 79, 562.CrossRefGoogle ScholarPubMed
Fahey, J. E. & Crawley, J. F. (1954). Studies on chronic respiratory disease of chickens. IV. A haemagglutination-inhibition diagnostic test. Can. J. comp. Med. 18, 264.Google Scholar
Feinberg, J. G. (1956). Agar clarification. Nature, Lond. 178, 1406.CrossRefGoogle ScholarPubMed
Hofstad, M. S. (1957). A serological study of infectious sinusitis in turkeys. Avian Dis. 1, 170.CrossRefGoogle Scholar
Hofstad, M. S. & Doerr, L. (1956). A chicken meat infusion medium enriched with avian serum for cultivation of an avian PPLO, Mycoplasma gallinarum. Cornell Vet. 46, 439.Google ScholarPubMed
Jerstad, A. C., Hamilton, C. M. & Smith, J. E. (1959). Egg transmission of infectious sinusitis in naturally infected turkeys. Avian Dis. 3, 28.CrossRefGoogle Scholar
Jungherr, E. L., Luginbuhl, R. E., Tourtellotte, M. & Burr, W. E. (1955). Significance of serological testing for chronic respiratory disease. Proc. Am. vet. med. Ass. Ann. Meeting, p. 315.Google Scholar
Leach, R. H. & Blaxland, J. D. (1966). The need for standardisation of serological techniques for the detection of M. gallisepticum infection in poultry. Vet. Rec. 79, 308.CrossRefGoogle Scholar
Newnham, A. G. (1964). The haemagglutination-inhibition (HI) test and a study of its use in experimental avian respiratory mycoplasmosis. Res. vet. Sci. 5, 245.CrossRefGoogle Scholar
Rhoades, K. R., Kelton, W. H. & Heddleston, K. L. (1965). Serological, pathologic and symptomatic aspects of mycoplasmosis of turkeys. Can. J. comp. Med. 29, 169.Google ScholarPubMed
Roberts, D. H. (1964 a). Experimental infection of chickens with Mycoplasma gallisepticum and subsequent re-isolation of the organism from the body tissues. Vet. Rec. 76, 798.Google Scholar
Roberts, D. H. (1964 b). Immunological aspects of infectious sinusitis in turkeys. Vet. Rec. 76, 1200.Google Scholar
Taylor-Robinson, D., Purcell, R. H., Wong, D. C. & Chanock, R. M. (1966). A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg., Camb. 64, 91.CrossRefGoogle ScholarPubMed